Method for detecting the methylation of colorectal-cancer-specific methylation marker genes for colorectal cancer diagnosis

ABSTRACT

The present disclosure relates to a method for detecting CpG methylation of SDC2 (Syndecan 2) gene, a kit for detecting CpG methylation of SDC2 (Syndecan 2) gene, and a method for detecting CpG methylation of SDC2 (Syndecan 2) gene for a colorectal cancer diagnosis.

CROSS-REFERENCE TO RELATED APPLICATIONS

This is a continuation-in-part application under 35 U.S.C. § 120 of U.S.patent application Ser. No. 13/508,534 filed May 7, 2012 and publishedas U.S. Patent Application Publication No. 2012/0264640, which in turnis a U.S. national phase under the provisions of 35 U.S.C. § 371 ofInternational Patent Application No. PCT/KR2010/007030 filed Oct. 14,2010, which in turn claims priority of Korean Patent Application No.10-2009-0106445 filed Nov. 5, 2009. The disclosures of U.S. patentapplication Ser. No. 13/508,534, International Patent Application No.PCT/KR2010/007030, and Korean Patent Application No. 10-2009-0106445 arehereby incorporated herein by reference in their respective entireties,for all purposes.

TECHNICAL FIELD

The present disclosure relates to a method for detecting CpG methylationof SDC2 (Syndecan 2) gene, a kit for detecting CpG methylation of SDC2(Syndecan 2) gene, and a method for detecting CpG methylation of SDC2(Syndecan 2) gene for a colorectal cancer diagnosis.

BACKGROUND ART

Even at the present time when medical science has advanced, the 5-yearsurvival rate of cancer patients, particularly solid tumor patients(other than blood cancer patients) is less than 50%, and about ⅔ of allcancer patients are diagnosed at an advanced stage and almost all diewithin 2 years after cancer diagnosis. Such poor results in cancertherapy are not only the problem of therapeutic methods, but also due tothe fact that it not easy to diagnose cancer at an early stage and toaccurately diagnose advanced cancer and to carry out the follow-up ofcancer patients after cancer therapy.

In current clinical practice, the diagnosis of cancer is confirmed byperforming tissue biopsy after history taking, physical examination andclinical assessment, followed by radiographic testing and endoscopy ifcancer is suspected. However, the diagnosis of cancer by the existingclinical practices is possible only when the number of cancer cells ismore than a billion and the diameter of cancer is more than 1 cm. Inthis case, the cancer cells already have metastatic ability, and atleast half thereof have already metastasized. Meanwhile, tumor markersfor monitoring substances that are directly or indirectly produced fromcancers are used in cancer screening, but they cause confusion due tolimitations in accuracy, since up to about half thereof appear normaleven in the presence of cancer, and they often appear positive even inthe absence of cancer. Furthermore, the anticancer agents that aremainly used in cancer therapy have the problem that they show an effectonly when the volume of cancer is small.

The reason why the diagnosis and treatment of cancer are difficult isthat cancer cells are highly complex and variable. Cancer cells growexcessively and continuously, invading surrounding tissue andmetastasize to distal organs leading to death. Despite the attack of animmune mechanism or anticancer therapy, cancer cells survive,continually develop, and cell groups that are most suitable for survivalselectively propagate. Cancer cells are living bodies with a high degreeof viability, which occur by the mutation of a large number of genes. Inorder that one cell is converted to a cancer cell and developed to amalignant cancer lump that is detectable in clinics, the mutation of alarge number of genes must occur. Thus, in order to diagnose and treatcancer at the root, approaches at a gene level are necessary.

Recently, genetic analysis has been actively attempted to diagnosecancer. The simplest typical method is to detect the presence of ABL:BCR fusion genes (the genetic characteristic of leukemia) in blood byPCR. The method has an accuracy rate of more than 95%, and after thediagnosis and therapy of chronic myelocytic leukemia using this simpleand easy genetic analysis, this method is being used for the assessmentof the result and follow-up study. However, this method has thedeficiency that it can be applied only to some blood cancers.

Furthermore, another method has been attempted, in which the presence ofgenes expressed by cancer cells is detected by RT-PCR and blotting,thereby diagnosing cancer cells present in blood cells. However, thismethod has shortcomings in that it can be applied only to some cancers,including prostate cancer and melanoma, has a high false positive rate.In addition, it is difficult to standardize detection and reading inthis method, and its utility is also limited (Kopreski, M. S. et al.,Clin. Cancer Res., 5:1961, 1999; Miyashiro, I. et al., Clin. Chem.,47:505, 2001).

Accordingly, methods of diagnosing cancer by measuring DNA methylationhave recently been proposed. When the promoter CpG island of a certaingene is hyper-methylated, the expression of such a gene is silenced.This is interpreted to be a main mechanism by which the function of thisgene is lost even when there is no mutation in the protein-codingsequence of the gene in a living body. In addition, this is analyzed asa factor by which the function of a number of tumor-suppressor genes inhuman cancer is lost. Thus, analysis of the methylation of the promoterCpG island of tumor-suppressor genes is very helpful in cancer research.An active attempt has been made to analyze the methylation of thepromoter CpG island by methods such as methylation-specific PCR(hereinafter, referred to as “MSP”) or automatic base sequencing and touse the analysis results for the diagnosis and screening of cancer.

A significant number of diseases are caused by genetic abnormalities,and the most frequent form of genetic abnormality is a change in thecoding sequence of a gene. This genetic change is referred to asmutation. When any gene has a mutation, the structure and function of aprotein encoded by the gene change, resulting in abnormalities anddeletions, and this mutant protein causes disease. However, anabnormality in the expression of a specific gene can cause disease evenin the absence of a mutation in the gene. A typical example thereof ismethylation in which a methyl group is attached to the transcriptionregulatory region of a gene, that is, the cytosine base of the promoterCpG islands, and in this case, the expression of the gene is silenced.This is known as epigenetic change. This is transmitted to offspring andresults in the loss of the expression of the relevant protein in thesame manner as mutation. Most typically, the expression of tumorsuppressor genes is silenced by the methylation of promoter CpG islandsin cancer cells, resulting in carcinogenesis (Robertson, K. D. et al.,Carcinogensis, 21:461, 2000).

For the accurate diagnosis of cancer, it is important to detect not onlya mutated gene but also a mechanism by which the mutation of this geneoccurs. In recent years, epigenetic changes were reported to be asimportant as these mutations, and a typical example of the epigeneticchanges is the methylation of promoter CpG islands.

In the genomic DNA of mammal cells, there is the fifth base in additionto A, C, G and T, namely, 5-methylcytosine, in which a methyl group isattached to the fifth carbon of the cytosine ring (5-mC). 5-mC is alwaysattached only to the C of a CG dinucleotide (5′-mCG-3′), which isfrequently marked CpG. The C of CpG is mostly methylated by attachmentwith a methyl group. The methylation of this CpG inhibits a repetitivesequence in genomes, such as Alu or transposon, from being expressed. Inaddition, this CpG is a site where an epigenetic change in mammaliancells appears most often. The 5-mC of this CpG is naturally deaminatedto T, and thus, the CpG in mammal genomes shows only 1% of frequency,which is much lower than a normal frequency (1/4×1/4=6.25%).

Regions in which CpG are exceptionally integrated are known as CpGislands. The CpG islands refer to sites which are 0.2-3 kb in length,and have a C+G content of more than 50% and a CpG ratio of more than3.75%. There are about 45,000 CpG islands in the human genome, and theyare mostly found in promoter regions regulating the expression of genes.Actually, the CpG islands occur in the promoters of housekeeping genesaccounting for about 50% of human genes (Cross, S. et al., Curr. Opin.Gene Develop., 5:309, 1995).

In the meantime, in the somatic cells of normal persons, the CpG islandsof such housekeeping gene promoter sites are un-methylated, butimprinted genes and the genes on inactivated X chromosomes aremethylated such that they are not expressed during development.

During a cancer-causing process, methylation is found in promoter CpGislands, and the restriction on the corresponding gene expressionoccurs. Particularly, if methylation occurs in the promoter CpG islandsof tumor-suppressor genes that regulate cell cycle or apoptosis, restoreDNA, are involved in the adhesion of cells and the interaction betweencells, and/or suppress cell invasion and metastasis, such methylationblocks the expression and function of such genes in the same manner asthe mutations of a coding sequence, thereby promoting the developmentand progression of cancer. In addition, partial methylation also occursin the CpG islands according to aging.

An interesting fact is that, in the case of genes whose mutations areattributed to the development of cancer in congenital cancer but do notoccur in acquired cancer, the methylation of promoter CpG islands occursinstead of mutation. Typical examples include the promoter methylationof genes, such as acquired renal cancer VHL (von Hippel Lindau), breastcancer BRCA1, colorectal cancer MLH1, and stomach cancer E-CAD. Inaddition, in about half of all cancers, the promoter methylation of p16or the mutation of Rb occurs, and the remaining cancers show themutation of p53 or the promoter methylation of p73, p 14 and the like.

An important fact is that an epigenetic change caused by promotermethylation causes a genetic change (i.e., the mutation of a codingsequence), and the development of cancer is progressed by thecombination of such genetic and epigenetic changes. In a MLH1 gene as anexample, there is the circumstance in which the function of one alleleof the MLH1 gene in colorectal cancer cells is lost due to its mutationor deletion, and the remaining one allele does not function due topromoter methylation. In addition, if the function of MLH1, which is aDNA restoring gene, is lost due to promoter methylation, the occurrenceof mutation in other important genes is facilitated to promote thedevelopment of cancer.

Most cancers show three common characteristics with respect to CpG,namely, hypermethylation of the promoter CpG islands of tumor-suppressorgenes, hypomethylation of the remaining CpG base sites, and an increasein the activity of methylation enzyme, namely, DNA cytosinemethyltransferase (DNMT) (Singal, R. & Ginder, G. D., Blood, 93:4059,1999; Robertson, K. et al., Carcinogensis, 21:461, 2000; Malik, K. &Brown, K. W., Brit. J. Cancer, 83:1583, 2000).

When promoter CpG islands are methylated, the reason why the expressionof the corresponding genes is blocked is not clearly established, but ispresumed to be because a methyl CpG-binding protein (MECP) or a methylCpG-binding domain protein (MBD), and histone deacetylase, bind tomethylated cytosine, thereby causing a change in the chromatin structureof chromosomes and a change in histone protein.

It is unsettled whether the methylation of promoter CpG islands directlycauses the development of cancer or is a secondary change after thedevelopment of cancer. However, it is clear that the promotermethylation of tumor-related genes is an important index to cancer, andthus can be used in many applications, including the diagnosis and earlydetection of cancer, the prediction of the risk of the development ofcancer, the prognosis of cancer, follow-up examination after treatment,and the prediction of a response to anticancer therapy. Recently, anattempt to examine the promoter methylation of tumor-related genes intissues, cells, blood, sputum, saliva, feces or urine and to use theexamined results for the diagnosis and treatment of various cancers, hasbeen actively conducted (Esteller, M. et al., Cancer Res., 59:67, 1999;Sanchez-Cespedez, M. et al., Cancer Res., 60:892, 2000; Ahlquist, D. A.et al., Gastroenterol., 119:1219, 2000).

In order to maximize the accuracy of cancer diagnosis using promotermethylation, analyze the development of cancer according to each stageand discriminate a change according to cancer and aging, an examinationthat can accurately analyze the methylation of all the cytosine bases ofpromoter CpG islands is required. Currently, a standard method for thisexamination is a bisulfite genome-sequencing method, in which a sampleDNA is treated with sodium bisulfite, and all regions of the CpG islandsof a target gene to be examined is amplified by PCR, and then, the basesequence of the amplified regions is analyzed. However, this examinationhas the problem that there are limitations to the number of genes orsamples that can be examined at a given time. Other problems are thatautomation is difficult, and much time and expense are required.

In the Johns Hopkins School of Medicine, the MD Anderson Cancer Center,Charité-Universitätsmedizin Berlin, etc., studies on promotermethylation of cancer-related genes have been actively conducted. Thefundamental data thus obtained are interchanged through the DNAMethylation Society (DMS) and stored in MethDB (www.methdb.de).Meanwhile, EpiGenX Pharmaceuticals, Inc. is now developing therapeuticagents associated with the methylation of CpG islands, and Epigenomics,Inc. is now conducting studies to apply promoter methylation to cancerdiagnosis by examining the promoter methylation using varioustechniques, such as DNA chips and MALDI-TOF.

Accordingly, the present inventors have made extensive efforts todevelop an effective colorectal-cancer-specific methylation marker whichmakes it possible to diagnose cancer and the risk of carcinogenesis atan early stage and predict cancer prognosis. The present inventors haveinitially identified that SDC2 (NM_002998, Syndecan 2) gene, which isinvolved in cell migration, differentiation and proliferation, ismethylated in colorectal cancer (Oh et al., J. Mol. Diag. 2013). Thepresent inventors found candidate genes, which are hypermethylated incolorectal cancer tissues compared to normal tissues, by isolatingmethylated DNA from colon cancer tissues and normal tissues connected tocolon cancer tissues from 12 colorectal cancer patients under stage I tostage IV and followed by DNA microarray analysis. After a series ofverification processes, SDC2 was investigated as a promising methylationbiomarker for early diagnosis of colorectal cancer. Through a clinicalexamination using tissues of 139 colorectal cancer patients, 97.8% ofcolon cancer tissues show hypermethylation, when comparing tomethylation in normal tissues connected to colon cancer tissues,irrespective of stage. Sensitivity for the diagnosis of colorectalcancer was confirmed to 87% and specificity was confirmed to 95.2% inclinical examination using quantitative methylation-specific PCR forsera of 131 colon cancer patients under stage I to stage IV and 125healthy subjects. Especially, the sensitivity for stage I was 92.3%,which means that the biomarker found by the inventors was useful inearly diagnosis of colorectal cancer.

Under the current technical background, the inventors of the presentapplication have completed the invention by confirming that themethylation of CpG island of SDC2 (Syndecan 2) gene could be detectedwith high sensitivity and specificity with primers comprising one ormore CG, which are bound complementarily to the methylated SDC2 DNA.

As a result, the present inventors have found that SDC2 (NM_002998,Syndecan 2) is methylated specifically in colorectal cancer cells andthat colorectal cancer can be diagnosed by measuring the degree ofmethylation using these genes as biomarkers, thereby completing thepresent disclosure.

DISCLOSURE OF INVENTION

To achieve the above objects, the present disclosure provides a methodfor detecting CpG methylation of SDC2 (Syndecan 2) gene, the methodcomprising the steps of: (a) isolating genomic DNA from a clinicalsample; (b) treating the genomic DNA from step (a) with bisulfite; and(c) determining hypermethylation of the CpG of the SDC2 gene in thegenomic DNA treated with bisulfite according the step (b) by usingprimer(s) to amplify a methylated CpG of the bisulfite-treated SDC2gene.

The present disclosure also provides a kit for detecting CpG methylationof SDC2 (Syndecan 2) gene, comprising primer(s) to amplify a methylatedCpG of the SDC2 gene.

The present disclosure also provides a method for detecting CpGmethylation of SDC2 (Syndecan 2) gene for a colorectal cancer diagnosis,the method comprising the steps of: (a) isolating genomic DNA from aclinical sample; (b) treating the genomic DNA from step (a) withbisulfite; and (c) determining hypermethylation of the CpG of the SDC2gene in the genomic DNA treated with bisulfite according the step (b) byusing primer(s) to amplify a methylated CpG of the bisulfite-treatedSDC2 gene, wherein a colorectal cancer is detected in the human subjectbased on increased CpG methylation of the SDC2 gene relative to that ofa control.

Other features and embodiments of the present disclosure will be moreapparent from the following detailed descriptions and the appendedclaims.

Effects of the Invention

According to the method for detecting CpG island methylation of SDC2gene of the present disclosure, the CpG island methylation of SDC2 genecan be detected in a clinical sample at a high detection rate in anaccurate, rapid and efficient manner.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 is a graph diagram showing a detection rate at which SDC2 genemethylation is detected in various specimens using 808 sets of primersand probes used in the method according to the present disclosure.

FIG. 2 is a graph diagram showing a degree of methylation of the SDC2gene in fecal samples of normal persons and colorectal patients using808 sets of primers and probes used in the method according to thepresent disclosure.

FIG. 3 is a graph diagram showing a degree of methylation of the SDC2gene in serum samples of normal persons and colorectal patients using808 sets of primers and probes used in the method according to thepresent disclosure.

BEST MODE FOR CARRYING OUT THE INVENTION

Unless defined otherwise, all technical and scientific terms used hereinhave the same meaning as commonly understood by one of ordinary skill inthe art to which the invention pertains. Generally, the nomenclaturesused herein are well known and are commonly employed in the art.

The present disclosure is characterized in that the CpG islands of SDC2(NM_002998, Syndecan 2) gene, which is methylated specifically incolorectal cancer cells, are used as a biomarker.

In one aspect, the present disclosure is directed to method fordetecting CpG methylation of SDC2 (Syndecan 2) gene, the methodcomprising the steps of:

(a) isolating genomic DNA from a clinical sample;

(b) treating the genomic DNA from step (a) with bisulfite; and

(c) determining hypermethylation of the CpG of the SDC2 gene in thegenomic DNA treated with bisulfite according the step (b) by usingprimer(s) to amplify a methylated CpG of the bisulfite-treated SDC2gene.

As used herein, the term “sample”, “clinical sample”, or “specimen” ismeant to include any biological body fluid, in its broadest sense,obtained from an individual, body fluid, a cell line, a tissue culture,depending on the type of assay that is to be performed. For example, thebiological body fluid includes feces, blood, serum, plasma, and urine.It also includes cell, feces, urine, sputum, cell separated and flowedout from bronchoalveolar lavage fluid, paraffin tissue, and fine needleaspiration biopsy specimen. In other words, the clinical sample may beselected from the group consisting of, for example, tissue, biopsy,paraffin tissue, blood, serum, plasma, fine needle aspiration biopsyspecimen, cell, feces, urine, sputum, cell separated and flowed out frombronchoalveolar lavage fluid, and combinations thereof, which arederived from a patient suspected of cancer or a subject to be diagnosed,but is not limited thereto. Methods for obtaining tissue biopsies andbody fluids from mammals are well known in the art.

DNA is isolated from the clinical sample. DNA isolation may be performedusing, for example, magnetic particles. Specifically, magnetic particlesare allowed to bind to DNA in the clinical sample, and then an externalmagnetic field is applied to the sample to thereby isolate the DNA. Themagnetic particles that are used in DNA isolation may have a particlesize of about 50 to 2000 nm. The isolation of DNA from the clinicalsample may be performed using any one of various DNA isolation kits orDNA isolation reagents similar thereto, which are commercialized andsupplied.

For example, the isolated DNA may be treated with a reagent bisulfite,thereby modifying methylated DNA and unmethylated DNA differently. Thegenomic DNA nucleotide sequence of SDC2 gene CpG islands that can bemethylated is represented by SEQ ID NO: 1. When the nucleotide sequenceof SEQ ID NO: 1 is artificially modified by treatment with a reagent(e.g., bisulfite) that modifies methylated DNA and unmethylated DNAdifferently, cytosine bases methylated by the reagent may remain intact,and unmethylated cytosine bases may be converted to uracil or basesother than cytosine. Specifically, a nucleotide sequence correspondingto methylated SDC2 DNA is set forth in SEQ ID NO: 2.

In the present disclosure, the CpG islands may be located in theregulatory region including a promoter region, coding regions (e.g.,exons), downstream of coding regions for example, enhancer region, andintron region of the genes.

Herein, the intron region of the SDC2 gene may be located between +681and +1800 nucleotides (nt) from the transcription start site and maycomprise a nucleotide sequence of SEQ ID NO: 843.

In the present disclosure, step (c) may be performed by a methodselected from the group consisting of PCR, methylation-specific PCR,real-time methylation-specific PCR, PCR assay using a methylationDNA-specific binding protein, quantitative PCR, DNA chip-based assay,pyrosequencing, and bisulfite sequencing.

In the present disclosure, the method for detection of methylation is asfollows:

(1) Methylation-specific PCR: When genomic DNA is treated withbisulfite, cytosine in the 5′-CpG′-3 region remains intact, if it wasmethylated, but the cytosine changes to uracil, if it was unmethylated.Accordingly, based on the base sequence converted after bisulfitetreatment, PCR primer sets corresponding to a region having the5′-CpG-3′ base sequence are constructed. Herein, the constructed primersets are two kinds of primer sets: a primer set corresponding to themethylated base sequence, and a primer set corresponding to theunmethylated base sequence. When genomic DNA is converted with bisulfiteand then amplified by PCR using the above two kinds of primer sets, thePCR product is detected in the PCR mixture employing the primerscorresponding to the methylated base sequence, if the genomic DNA wasmethylated, but the genomic DNA is detected in the PCR mixture employingthe primers corresponding to the unmethylated, if the genomic DNA wasunmethylated. This methylation can be quantitatively analyzed by agarosegel electrophoresis.

(2) Real-time methylation specific PCR: Real-time methylation-specificPCR is a real-time measurement method modified from themethylation-specific PCR method and comprises treating genomic DNA withbisulfite, designing PCR primers corresponding to the methylated basesequence, and performing real-time PCR using the primers. Methods ofdetecting the methylation of the genomic DNA include two methods: amethod of detection using a TanMan probe complementary to the amplifiedbase sequence; and a method of detection using Sybergreen. Thus, thereal-time methylation-specific PCR allows selective quantitativeanalysis of methylated DNA. Herein, a standard curve is plotted using anin vitro methylated DNA sample, and a gene containing no 5′-CpG-3′sequence in the base sequence is also amplified as a negative controlgroup for standardization to quantitatively analyze the degree ofmethylation.

(3) Pyrosequencing: The pyrosequencing method is a quantitativereal-time sequencing method modified from the bisulfite sequencingmethod. Similarly to bisulfite sequencing, genomic DNA is converted bybisulfite treatment, and then, PCR primers corresponding to a regioncontaining no 5′-CpG-3′ base sequence are constructed. Specifically, thegenomic DNA is treated with bisulfite, amplified using the PCR primers,and then subjected to real-time base sequence analysis using asequencing primer. The degree of methylation is expressed as amethylation index by analyzing the amounts of cytosine and thymine inthe 5′-CpG-3′ region.

(4) PCR Using Methylated DNA-specific binding protein, quantitative PCR,and DNA Chip Assay: When a protein binding specifically only tomethylated DNA is mixed with DNA, the protein binds specifically only tothe methylated DNA. Thus, either PCR using a methylation-specificbinding protein or a DNA chip assay allows selective isolation of onlymethylated DNA. Genomic DNA is mixed with a methylation-specific bindingprotein, and then only methylated DNA was selectively isolated. Theisolated DNA is amplified using PCR primers corresponding to thepromoter region, and then methylation of the DNA is measured by agarosegel electrophoresis.

In addition, methylation of DNA can also be measured by a quantitativePCR method, and methylated DNA isolated with a methylated DNA-specificbinding protein can be labeled with a fluorescent probe and hybridizedto a DNA chip containing complementary probes, thereby measuringmethylation of the DNA. Herein, the methylated DNA-specific bindingprotein may be, but not limited to, McrBt.

(5) Detection of Differential Methylation—Methylation-SensitiveRestriction Endonuclease: Detection of differential methylation can beaccomplished by bringing a nucleic acid sample into contact with amethylation-sensitive restriction endonuclease that cleaves onlyunmethylated CpG sites.

In a separate reaction, the sample is further brought into contact withan isoschizomer of the methylation-sensitive restriction enzyme thatcleaves both methylated and unmethylated CpG-sites, thereby cleaving themethylated nucleic acid.

Specific primers are added to the nucleic acid sample, and the nucleicacid is amplified by any conventional method. The presence of anamplified product in the sample treated with the methylation-sensitiverestriction enzyme but absence of an amplified product in the sampletreated with the isoschizomer of the methylation-sensitive restrictionenzyme indicates that methylation has occurred at the nucleic acidregion assayed. However, the absence of an amplified product in thesample treated with the methylation-sensitive restriction enzymetogether with the absence of an amplified product in the sample treatedwith the isoschizomer of the methylation-sensitive restriction enzymeindicates that no methylation has occurred at the nucleic acid regionassayed.

As used herein, the term “methylation-sensitive restriction enzyme”refers to a restriction enzyme (e.g., SmaI) that includes CG as part ofits recognition site and has activity when the C is methylated ascompared to when the C is not methylated. Non-limiting examples ofmethylation-sensitive restriction enzymes include MspI, HpaII, BssHII,BstUI and NotI. Such enzymes can be used alone or in combination.Examples of other methylation-sensitive restriction enzymes include, butare not limited to SacII and EagI.

The isoschizomer of the methylation-sensitive restriction enzyme is arestriction enzyme that recognizes the same recognition site as themethylation-sensitive restriction enzyme but cleaves both methylated andunmethylated CGs. An example thereof includes MspI.

Primers of the present disclosure are designed to be “substantially”complementary to each strand of the locus to be amplified and includethe appropriate G or C nucleotides as discussed above. This means thatthe primers must be sufficiently complementary to hybridize with theirrespective strands under polymerization reaction conditions. Primers ofthe present disclosure are used in the amplification process, which isan enzymatic chain reaction (e.g., PCR) in which that a target locusexponentially increases through a number of reaction steps. Typically,one primer is homologous with the negative (−) strand of the locus(antisense primer), and the other primer is homologous with the positive(+) strand (sense primer). After the primers have been annealed todenatured nucleic acid, the nucleic acid chain is extended by an enzymesuch as DNA Polymerase I (Klenow), and reactants such as nucleotides,and, as a result, + and − strands containing the target locus sequenceare newly synthesized. When the newly synthesized target locus is usedas a template and subjected to repeated cycles of denaturing, primerannealing, and extension, exponential synthesis of the target locussequence occurs. The resulting reaction product is a discrete nucleicacid duplex with termini corresponding to the ends of specific primersemployed.

The amplification reaction is PCR which is commonly used in the art.However, alternative methods such as real-time PCR or linearamplification using isothermal enzyme may also be used. In addition,multiplex amplification reactions may also be used.

(6) Detection of Differential Methylation—Bisulfite Sequencing Method:Another method for detecting a methylated CpG-containing nucleic acidcomprises the steps of: bringing a nucleic acid-containing sample intocontact with an agent that modifies unmethylated cytosine; andamplifying the CpG-containing nucleic acid in the sample usingCpG-specific oligonucleotide primers, wherein the oligonucleotideprimers distinguish between modified methylated nucleic acid andnon-methylated nucleic acid and detect the methylated nucleic acid. Theamplification step is optional and desirable, but not essential. Themethod relies on the PCR reaction to distinguish between modified (e.g.,chemically modified) methylated DNA and unmethylated DNA.

In the present disclosure, the primer(s) is, for example, 10-40 meroligonucleotides that are complementary to methylated SDC2 DNA so as tobe capable of amplifying the methylated SDC2 DNA. The primers may bedesigned to be “substantially” complementary to each strand of the locusto be amplified of a target DNA. This means that the primers must besufficiently complementary to hybridize with their respective strandsunder polymerization reaction conditions.

The primers include forward and/or reverse primers, and the forwardand/or reverse primers include one or more CGs or GCs.

Specifically, the forward primer may bind to a sequence complementary tothe sequence of SEQ ID NO: 2 to specifically amplify the sequencecomplementary to the sequence of SEQ ID NO: 2, and may contain cytosine(C) at the 3′ end. For example, the sequence of the methylated strand(sense strand) of SDC2 gene, converted by bisulfite, is set forth in SEQID NO: 2, and the forward primer can be designed to bind to a sequencecomplementary to the sequence of SEQ ID NO: 2 and to end with “C” of CGat the 3′ end so as to more differentiate between methylated (“C”) andunmethylated (“U”, “T”) SDC2 genes.

The reverse primer may bind to the nucleotide sequence of SEQ ID NO: 2to amplify the nucleotide sequence of SEQ ID NO: 2, and may containguanine G at the 3′ end. For example, the reverse primer can be designedto bind complementarily to SEQ ID NO: 2 and contain guanine G at the 3′end so that the directionality thereof is opposite to that of theforward primer.

The reverse primer is primarily bind to the sequence of SEQ ID NO: 2 asa template to amplify the sequence of SEQ ID NO: 2, and the forwardprimer is secondarily bind to the amplified sequence so as to enableamplification of the sequence.

Specifically, the primer may comprise a primer pair including forwardand reverse primers. For example, the primer may comprise a sequencehaving a homology of at least 80%, specifically at least 81%, 82%, 83%,84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%,98% or 99%, to one or more sequences selected from the group consistingof SEQ ID NOs: 3, 4, 6-67, 69-100, 102-153, 155-216, 218-279, 281-342,344-395, 397-448, 450-511, 513-574, 576-637, 639-700, 702-763, 765-826,and 828-841, but is not limited thereto. For example, the primer maycomprise one or more sequences selected from the group consisting of SEQID NOs: 3, 4, 6-67, 69-100, 102-153, 155-216, 218-279, 281-342, 344-395,397-448, 450-511, 513-574, 576-637, 639-700, 702-763, 765-826 and828-841.

The forward primer may comprise a sequence having a homology of at least80%, specifically at least 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%,90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99%, to one or moresequences selected from the group consisting of SEQ ID NOs: 3, 6-65, 66,69-98, 99, 102-151, 152, 155-214, 215, 218-277, 278, 281-340, 341,344-393, 394, 397-446, 447, 450-509, 510, 513-572, 573, 576-635, 636,639-698, 699, 702-761, 762, 765-824, 825, 828-840.

The reverse primer may comprise a sequence having a homology of at least80%, specifically at least 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%,90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99%, to one or moresequences selected from the group consisting of SEQ ID NOs: 4, 67, 100,153, 216, 279, 342, 395, 448, 511, 574, 637, 700, 763, 826 and 841.

Specifically, the primer may comprise, for example, forward and reverseprimers listed in Table 2 below.

The primer may comprise nucleotides subjected to either one modificationor a combination of two or more modifications selected from among:modification in which an OH group at the 2′ carbon position of a sugarstructure in one or more nucleotides is substituted with —CH₃ (methyl),—OCH₃ (methoxy), —NH₂, —F (fluorine), —O-2-methoxyethyl-O-propyl,—O-2-methylthioethyl, —O-3-aminopropyl, —O-3-dimethylaminopropyl,—O—N-methylacetamido or —O-dimethylamidooxyethyl; modification in whichoxygen in a sugar structure in nucleotides is substituted with sulfur;modification in which oxygen in a sugar structure in nucleotides issubstituted with sulfur; and modification of a bond between nucleotidesto a phosphorothioate, boranophosphophate or methyl phosphonate bond, orsubjected to modification to PNA (peptide nucleic acid), LNA (lockednucleic acid), UNA (unlocked nucleic acid), or inosine. Alternatively,the primer may comprise one or more nucleotides subjected tomodification to 2′-5′ phosphodiester linkage.

In some embodiments, the method may comprise a step of detectingmethylation of target DNA by use of a self-reporting or energytransfer-labeled primer.

As used herein, the term “self-reporting” is also named “energy transferlabeled” and may be used interchangeably with “energy transfer labeled”.As used herein, “self-reporting universal primer” may be usedinterchangeably with the term “energy transfer labeled primer”.

“Self-reporting” or “energy transfer labeled” means that the primer iscapable of self-quenching or self-probing such that when amplificationdoes not occur, fluorescence is not emitted due to self-quenching, butwhen amplification occurs, quenching is released and fluorescence isemitted. Self-reporting or energy transfer-labeled substances include,but are not limited to, TaqMan probes, fluorophores and molecularbeacons.

In some embodiments, the primer may further comprise a probe capable ofhybridizing to the methylated SDC2 DNA to determine whether or not aproduct amplified with the primer would be produced.

A product amplified with the primer may be detected using any probe thatcan hybridize to, for example, target DNA to detect methylation. Forexample, the probe may contain one or more CpG dinucleotides. The probemay comprise a sequence having a homology of at least 80%, specificallyat least 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%,93%, 94%, 95%, 96%, 97%, 98% or 99%, to one or more sequences selectedfrom the group consisting of SEQ ID NOs: 5, 68, 101, 154, 217, 280, 343,396, 449, 512, 575, 638, 701, 764, 827 and 842, but is not limitedthereto. The probe may comprise one or more sequences selected from thegroup consisting of SEQ ID NOs: 5, 68, 101, 154, 217, 280, 343, 396,449, 512, 575, 638, 701, 764, 827 and 842. Specifically, the probe maycomprise, for example, probes listed in Table 2 below.

The reverse primer is primarily bind to the sequence of SEQ ID NO: 2 asa template to amplify the sequence of SEQ ID NO: 2, and the forwardprimer and the probe is secondarily bind to the amplified sequence sothat while amplification of the sequence proceeds, a signal by the probeor fluorescent dye can be emitted.

In nucleic acid hybridization reactions, the conditions used to achievea particular level of stringency will vary depending on the nature ofthe nucleic acids being hybridized. For example, the length, degree ofcomplementarity, nucleotide sequence composition (e.g., GC/AT content),and nucleic acid type (e.g., RNA/DNA) of the hybridizing regions of thenucleic acids can be considered in selecting hybridization conditions.An additional consideration is whether one of the nucleic acids isimmobilized, for example, on a filter.

An example of progressively higher stringency conditions is as follows:2×SSC/0.1% SDS at room temperature (hybridization conditions);0.2×SSC/0.1% SDS at room temperature (low stringency conditions);0.2×SSC/0.1% SDS at 42° C. (moderate stringency conditions); and 0.1×SSCat about 68° C. (high stringency conditions). Washing can be carried outusing only one of these conditions, e.g., high stringency conditions, oreach of the conditions can be used, e.g., for 10-15 minutes each, in theorder listed above, repeating any or all of the steps listed. However,as mentioned above, optimal conditions will vary depending on theparticular hybridization reaction involved, and can be determinedempirically. In general, conditions of high stringency are used for thehybridization of the probe of interest.

In some embodiments, the probe may have a reporter or a quencherattached to both ends. The reporter may be one or more selected from thegroup consisting of FAM (6-carboxyfluorescein), Texas red, HEX (2′, 4′,5′, 7′,-tetrachloro-6-carboxy-4,7-dichlorofluorescein), JOE, Cy3, andCy5. The quencher may be one or more selected from the group consistingof TAMRA (6-carboxytetramethyl-rhodamine), BHQ1, BHQ2 and Dabcyl. Thequencher may be one or more selected from the group consisting of TAMRA(6-carboxytetramethyl-rhodamine), BHQ1, BHQ2 and Dabcyl.

A method for screening methylation marker genes according to the presentdisclosure comprises the steps of: (a) isolating genomic DNAs fromtransformed cells and non-transformed cells; (b) reacting the isolatedgenomic DNAs with a methylated DNA-binding protein, thereby isolatingmethylated DNAs; and (c) amplifying the methylated DNAs, hybridizing theamplified DNAs to a CpG microarray, and then selecting genes showing thegreatest difference in the degree of methylation between the normalcells and the cancer cells, thereby ensuring methylation marker genes.

The above method for screening biomarker genes can find genes which aredifferentially methylated in colorectal cancer as well as at variousdysplasic stages of the tissue that progresses to colorectal cancer. Thescreened genes can be used for colorectal cancer screening,risk-assessment, prognosis, disease identification, the diagnosis ofdisease stages, and the selection of therapeutic targets.

The identification of genes that are methylated in colorectal cancer andabnormalities at various stages of colorectal cancer makes it possibleto diagnose colorectal cancer at an early stage in an accurate andeffective manner and allows methylation profiling of multiple genes andthe identification of new targets for therapeutic intervention.Furthermore, the methylation data according to the present disclosuremay be combined with other non-methylation related biomarker detectionmethods to obtain a more accurate system for colorectal cancerdiagnosis.

According to the method of the present disclosure, the progression ofcolorectal cancer at various stages or phases can be diagnosed bydetermining the methylation stage of one or more nucleic acid biomarkersobtained from a sample. By comparing the methylation stage of a nucleicacid isolated from a sample at each stage of colorectal cancer with themethylation stage of one or more nucleic acids isolated from a sample inwhich there is no cell proliferative disorder of colorectal tissue, aspecific stage of colorectal cancer in the sample can be detected.Herein, the methylation stage may be hypermethylation.

In one embodiment of the present disclosure, nucleic acid may bemethylated in the regulatory region of a gene. In another embodiment, agene which is involved in cell transformation can be diagnosed at anearly stage by detecting methylation outside of the regulatory region ofthe gene, because methylation proceeds inwards from the outside of thegene.

In yet another embodiment of the present disclosure, cells that arelikely to form colorectal cancer can be diagnosed at an early stageusing the methylation marker genes. When genes confirmed to bemethylated in cancer cells are methylated in cells that appear normalclinically or morphologically, this indicates that the normallyappearing cells progress to cancer. Thus, colorectal cancer can bediagnosed at an early stage by detecting the methylation of colorectalcancer-specific genes in cells that appear normal.

The use of the methylation marker gene of the present disclosure allowsfor detection of a cellular proliferative disorder (dysplasia) ofcolorectal tissue in a sample. The detection method comprises bringing asample comprising at least one nucleic acid isolated from a subject intocontact with at least one agent capable of determining the methylationstate of the nucleic acid. The method comprises detecting themethylation of at least one region in at least one nucleic acid, whereinthe methylation of the nucleic acid differs from the methylation stateof the same region of a nucleic acid present in a sample in which thereis no abnormal growth (dysplastic progression) of colorectal cells.

In yet another embodiment of the present disclosure, the likelihood ofprogression of tissue to colorectal cancer can be evaluated by examiningthe methylation of a gene which is specifically methylated in colorectalcancer, and determining the methylation frequency of tissue that islikely to progress to colorectal cancer.

Thus, in another aspect, the present disclosure is directed to a methodfor detecting CpG methylation of SDC2 (Syndecan 2) gene for a colorectalcancer diagnosis, the method comprising the steps of:

(a) isolating genomic DNA from a clinical sample;

(b) treating the genomic DNA from step (a) with bisulfite; and

(c) determining hypermethylation of the CpG of the SDC2 gene in thegenomic DNA treated with bisulfite according the step (b) by usingprimer(s) to amplify a methylated CpG of the bisulfite-treated SDC2gene, wherein a colorectal cancer is detected in the human subject basedon increased CpG methylation of the SDC2 gene relative to that of acontrol.

The method comprises determining the methylation status of SDC2 geneisolated from a sample, wherein the methylation status of the SDC2 geneis compared with the methylation stage of a SDC2 gene isolated from asample in which there is no abnormal growth (dysplastic progression) ofcolorectal cells.

In another aspect, the present disclosure is directed to a kit fordetecting CpG methylation of SDC2 (Syndecan 2) gene, comprisingprimer(s) to amplify a methylated CpG of the SDC2 gene.

The kit of the present disclosure makes it possible to determine theabnormal growth (dysplastic progression) of colorectal cells in asample.

As used herein, the term “early detection” of cancer refers todiscovering the likelihood of cancer prior to metastasis, and preferablybefore observation of a morphological change in a tissue or cell.Furthermore, the term “early detection” of cell transformation refers tothe high probability of a cell to undergo transformation in its earlystages before the cell is morphologically designated as beingtransformed.

As used herein, the term “hypermethylation” refers to the methylation ofa CpG island. Hypermethylation as used herein refers to the presence ofmethylated alleles in one or more nucleic acids. Nucleic acids from asubject not having a cellular proliferative disorder of colorectaltissue contain no detectable methylated alleles when the same nucleicacids are examined.

In the present disclosure, “normal” cells refer to those that do notshow any abnormal morphological or cytological changes. “Tumor” cellsare cancer cells. “Non-tumor” cells are those cells that are part of thediseased tissue but are not considered to be the tumor portion.

As used herein, “predisposition” refers to the property of beingsusceptible to a cellular proliferative disorder. A subject having apredisposition to a cellular proliferative disorder has no cellularproliferative disorder, but is a subject having an increased likelihoodof having a cellular proliferative disorder.

The term “nucleic acid” or “nucleic acid sequence” as used herein refersto an oligonucleotide, nucleotide or polynucleotide, or fragmentsthereof, or single-stranded or double-stranded DNA or RNA of genomic orsynthetic origin, sense- or antisense-strand DNA or RNA of genomic orsynthetic origin, peptide nucleic acid (PNA), or any DNA-like orRNA-like material of natural or synthetic origin. Typically, theCpG-containing nucleic acid is DNA. However, the inventive method mayemploy, for example, samples that contain DNA, or DNA and RNA containingmRNA, wherein DNA or RNA may be single-stranded or double-stranded, or aDNA-RNA hybrid may be included in the sample.

A mixture of nucleic acids in a single reactor (tube) may also be used.The specific nucleic acid sequence to be detected may be a fraction of alarger molecule or can be present initially as a discrete molecule, sothat the specific sequence constitutes the entire nucleic acid. It isnot necessary that the sequence to be studied be present initially in apure form; the nucleic acid may be a minor fraction of a complexmixture, such as contained in whole human DNA.

Nucleic acids isolated from a subject are obtained in a biologicalsample from the subject. If it is desired to detect colorectal cancer orstages of colorectal cancer progression, the nucleic acid may beisolated from colorectal tissue by scraping or biopsy. Such samples maybe obtained by various medical procedures known to those of skill in theart.

The present disclosure provides a kit useful for detecting CpGmethylation of SDC2 (Syndecan 2) gene, comprising primer(s) to amplify amethylated CpG of the SDC2 gene.

Specifically, the primer may comprise a primer pair including forwardand reverse primers. For example, the primer may comprise a sequencehaving a homology of at least 80%, specifically at least 81%, 82%, 83%,84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%,98% or 99%, to one or more sequences selected from the group consistingof SEQ ID NOs: 3, 4, 6-67, 69-100, 102-153, 155-216, 218-279, 281-342,344-395, 397-448, 450-511, 513-574, 576-637, 639-700, 702-763, 765-826,and 828-841, but is not limited thereto. For example, the primer maycomprise one or more sequences selected from the group consisting of SEQID NOs: 3, 4, 6-67, 69-100, 102-153, 155-216, 218-279, 281-342, 344-395,397-448, 450-511, 513-574, 576-637, 639-700, 702-763, 765-826 and828-841.

The forward primer may comprise a sequence having a homology of at least80%, specifically at least 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%,90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99%, to one or moresequences selected from the group consisting of SEQ ID NOs: 3, 6-65, 66,69-98, 99, 102-151, 152, 155-214, 215, 218-277, 278, 281-340, 341,344-393, 394, 397-446, 447, 450-509, 510, 513-572, 573, 576-635, 636,639-698, 699, 702-761, 762, 765-824, 825, 828-840.

The reverse primer may comprise a sequence having a homology of at least80%, specifically at least 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%,90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99%, to one or moresequences selected from the group consisting of SEQ ID NOs: 4, 67, 100,153, 216, 279, 342, 395, 448, 511, 574, 637, 700, 763, 826 and 841.

In some embodiments, the primer may further comprise a probe capable ofhybridizing to the methylated SDC2 DNA to determine whether or not aproduct amplified with the primer would be produced.

A product amplified with the primer may be detected using any probe thatcan hybridize to, for example, target DNA to detect methylation. Forexample, the probe may contain one or more CpG dinucleotides. The probemay comprise a sequence having a homology of at least 80%, specificallyat least 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%,93%, 94%, 95%, 96%, 97%, 98% or 99%, to one or more sequences selectedfrom the group consisting of SEQ ID NOs: 5, 68, 101, 154, 217, 280, 343,396, 449, 512, 575, 638, 701, 764, 827 and 842, but is not limitedthereto. The probe may comprise one or more sequences selected from thegroup consisting of SEQ ID NOs: 5, 68, 101, 154, 217, 280, 343, 396,449, 512, 575, 638, 701, 764, 827 and 842.

The kit of the present disclosure comprises a carrier meanscompartmentalized to receive a sample therein, one or more containerscomprising a second container containing primers for amplification of a5′-CpG-3′ base sequence of methylated SDC 2 gene. Alternatively, a thirdcontainer contains a probe for detecting an amplified product.

Carrier means are suited for containing one or more containers such asvials, tubes, and the like, each of the containers comprising one of theseparate elements to be used in the method. In view of the descriptionprovided herein of the inventive method, those of skill in the art canreadily determine the apportionment of the necessary reagents among thecontainers.

EXAMPLES

Hereinafter, the present disclosure will be described in further detailwith reference to examples. It will be obvious to a person havingordinary skill in the art that these examples are illustrative purposesonly and are not to be construed to limit the scope of the presentdisclosure.

Example 1: Detection of SDC2 Gene Methylation by Use of Methylated andUnmethylated Genomic DNAs

For detection of SDC2 gene methylation, 808 sets of methylation-specificprimers and probes (see Table 1) were designed, which are complementaryto the sequence of SEQ ID NO: 2 corresponding to the SDC2 sequence afterconversion by bisulfite. To test the abilities of these primers andprobes to detect SDC2 gene methylation, the EpiTect PCR Control DNA set(Qiagen, Cat. No. 59695) was used. The EpiTect PCR Control DNA set is aDNA set obtained by converting methylated and unmethylated human genomicDNAs by bisulfite. Using these genomic DNAs, methylation-specificreal-time PCR (qMSP) was performed using the 808 sets ofmethylation-specific primers and probes. The qMSP was performed using aRotor-Gene Q PCR system (Qiagen). Specifically, a total of 20 μL of PCRreaction solution (containing 2 μl of template DNA; 4 μL of 5×AptaTaqDNA Master (Roche Diagnostics); 2 μL (2 pmole/μL) of PCR primer, 2 L (2pmole/μL) of TaqMan probe; and 10 μL of D.W.) was prepared and subjectedto PCR under the following conditions: treatment at 95° C. for 5 min,and then 40 cycles, each consisting of 15 sec at 95° C. and 1 min atsuitable annealing temperature. Whether or not a PCR amplificationproduct would be produced was determined by measuring the cyclethreshold (C_(T)) value. The SDC2 gene methylation for each primer andprobe set was measured by the C_(T) value. It was determined that if theC_(T) value was detected in methylated genomic DNA, methylation wasnormally detected, and if the C_(T) value was not detected inunmethylated genomic DNA, the primer and probe set normally operated. Itwas shown that all the tested 808 sets of primers and probes normallydetected SDC2 gene methylation (Table 2).

Table 1. Primer and probe sequences for detection of SDC2 genemethylation

TABLE 1 Primer and probe sequences for detection of SDC2 gene methylationSize of amplified SEQ Set Primers Sequences (5′-->3′) product (bp)ID NO: F1 GGAGAGAGGAAAAG 140 3 R1 CACGCCGATTAACA 4 P1AGTCGCGGCGTTTATTGGTTTTCGGAGT 5 F2 GAGAGAGGAAAAGT 139 6 R1 CACGCCGATTAACA4 P1 AGTCGCGGCGTTTATTGGTTTTCGGAGT 5 F3 AGAGAGGAAAAGTG 138 7 R1CACGCCGATTAACA 4 P1 AGTCGCGGCGTTTATTGGTTTTCGGAGT 5 F4 GAGAGGAAAAGTGG 1378 R1 CACGCCGATTAACA 4 P1 AGTCGCGGCGTTTATTGGTTTTCGGAGT 5 F5AGAGGAAAAGTGGG 136 9 R1 CACGCCGATTAACA 4 P1 AGTCGCGGCGTTTATTGGTTTTCGGAGT5 F6 GAGGAAAAGTGGGG 135 10 R1 CACGCCGATTAACA 4 P1AGTCGCGGCGTTTATTGGTTTTCGGAGT 5 F7 AGGAAAAGTGGGGA 134 11 R1CACGCCGATTAACA 4 P1 AGTCGCGGCGTTTATTGGTTTTCGGAGT 5 F8 GGAAAAGTGGGGAG 13312 R1 CACGCCGATTAACA 4 P1 AGTCGCGGCGTTTATTGGTTTTCGGAGT 5 F9GAAAAGTGGGGAGA 132 13 R1 CACGCCGATTAACA 4 P1AGTCGCGGCGTTTATTGGTTTTCGGAGT 5 0 F10 AAAAGTGGGGAGAG 131 14 R1CACGCCGATTAACA 4 P1 AGTCGCGGCGTTTATTGGTTTTCGGAGT 5 1 F11 AAAGTGGGGAGAGA130 15 R1 CACGCCGATTAACA 4 P1 AGTCGCGGCGTTTATTGGTTTTCGGAGT 5 2 F12AAGTGGGGAGAGAA 129 16 R1 CACGCCGATTAACA 4 P1AGTCGCGGCGTTTATTGGTTTTCGGAGT 5 3 F13 AGTGGGGAGAGAAA 128 17 R1CACGCCGATTAACA 4 P1 AGTCGCGGCGTTTATTGGTTTTCGGAGT 5 4 F14 GTGGGGAGAGAAAG127 18 R1 CACGCCGATTAACA 4 P1 AGTCGCGGCGTTTATTGGTTTTCGGAGT 5 5 F15TGGGGAGAGAAAGG 126 19 R1 CACGCCGATTAACA 4 P1AGTCGCGGCGTTTATTGGTTTTCGGAGT 5 6 F16 GGGGAGAGAAAGGA 125 20 R1CACGCCGATTAACA 4 P1 AGTCGCGGCGTTTATTGGTTTTCGGAGT 5 7 F17 GGGAGAGAAAGGAA124 21 R1 CACGCCGATTAACA 4 P1 AGTCGCGGCGTTTATTGGTTTTCGGAGT 5 8 F18GGAGAGAAAGGAAG 123 22 R1 CACGCCGATTAACA 4 P1AGTCGCGGCGTTTATTGGTTTTCGGAGT 5 9 F19 GAGAGAAAGGAAGA 122 23 R1CACGCCGATTAACA 4 P1 AGTCGCGGCGTTTATTGGTTTTCGGAGT 5 0 F20 AGAGAAAGGAAGAA121 24 R1 CACGCCGATTAACA 4 P1 AGTCGCGGCGTTTATTGGTTTTCGGAGT 5 1 F21GAGAAAGGAAGAAA 120 25 R1 CACGCCGATTAACA 4 P1AGTCGCGGCGTTTATTGGTTTTCGGAGT 5 2 F22 AGAAAGGAAGAAAA 119 26 R1CACGCCGATTAACA 4 P1 AGTCGCGGCGTTTATTGGTTTTCGGAGT 5 3 F23 GAAAGGAAGAAAAG118 27 R1 CACGCCGATTAACA 4 P1 AGTCGCGGCGTTTATTGGTTTTCGGAGT 5 4 F24AAAGGAAGAAAAGG 117 28 R1 CACGCCGATTAACA 4 P1AGTCGCGGCGTTTATTGGTTTTCGGAGT 5 5 F25 AAGGAAGAAAAGGA 116 29 R1CACGCCGATTAACA 4 P1 AGTCGCGGCGTTTATTGGTTTTCGGAGT 5 6 F26 AGGAAGAAAAGGAT115 30 R1 CACGCCGATTAACA 4 P1 AGTCGCGGCGTTTATTGGTTTTCGGAGT 5 7 F27GGAAGAAAAGGATT 114 31 R1 CACGCCGATTAACA 4 P1AGTCGCGGCGTTTATTGGTTTTCGGAGT 5 8 F28 GAAGAAAAGGATTG 113 32 R1CACGCCGATTAACA 4 P1 AGTCGCGGCGTTTATTGGTTTTCGGAGT 5 9 F29 AAGAAAAGGATTGA112 33 R1 CACGCCGATTAACA 4 P1 AGTCGCGGCGTTTATTGGTTTTCGGAGT 5 0 F30AGAAAAGGATTGAG 111 34 R1 CACGCCGATTAACA 4 P1AGTCGCGGCGTTTATTGGTTTTCGGAGT 5 1 F31 GAAAAGGATTGAGA 110 35 R1CACGCCGATTAACA 4 P1 AGTCGCGGCGTTTATTGGTTTTCGGAGT 5 2 F32 AAAAGGATTGAGAA109 36 R1 CACGCCGATTAACA 4 P1 AGTCGCGGCGTTTATTGGTTTTCGGAGT 5 3 F33AAAGGATTGAGAAA 108 37 R1 CACGCCGATTAACA 4 P1AGTCGCGGCGTTTATTGGTTTTCGGAGT 5 4 F34 AAGGATTGAGAAAA 107 38 R1CACGCCGATTAACA 4 P1 AGTCGCGGCGTTTATTGGTTTTCGGAGT 5 5 F35 AGGATTGAGAAAAC106 39 R1 CACGCCGATTAACA 4 P1 AGTCGCGGCGTTTATTGGTTTTCGGAGT 5 6 F36GGATTGAGAAAACG 105 40 R1 CACGCCGATTAACA 4 P1AGTCGCGGCGTTTATTGGTTTTCGGAGT 5 7 F37 GATTGAGAAAACGT 104 41 R1CACGCCGATTAACA 4 P1 AGTCGCGGCGTTTATTGGTTTTCGGAGT 5 8 F38 ATTGAGAAAACGTA103 42 R1 CACGCCGATTAACA 4 P1 AGTCGCGGCGTTTATTGGTTTTCGGAGT 5 9 F39TTGAGAAAACGTAG 102 43 R1 CACGCCGATTAACA 4 P1AGTCGCGGCGTTTATTGGTTTTCGGAGT 5 0 F40 TGAGAAAACGTAGG 101 44 R1CACGCCGATTAACA 4 P1 AGTCGCGGCGTTTATTGGTTTTCGGAGT 5 1 F41 GAGAAAACGTAGGA100 45 R1 CACGCCGATTAACA 4 P1 AGTCGCGGCGTTTATTGGTTTTCGGAGT 5 2 F42AGAAAACGTAGGAG 99 46 R1 CACGCCGATTAACA 4 P1 AGTCGCGGCGTTTATTGGTTTTCGGAGT5 3 F43 GAAAACGTAGGAGT 98 47 R1 CACGCCGATTAACA 4 P1AGTCGCGGCGTTTATTGGTTTTCGGAGT 5 4 F44 AAAACGTAGGAGTT 97 48 R1CACGCCGATTAACA 4 P1 AGTCGCGGCGTTTATTGGTTTTCGGAGT 5 5 F45 AAACGTAGGAGTTT96 49 R1 CACGCCGATTAACA 4 P1 AGTCGCGGCGTTTATTGGTTTTCGGAGT 5 6 F46AACGTAGGAGTTTT 95 50 R1 CACGCCGATTAACA 4 P1 AGTCGCGGCGTTTATTGGTTTTCGGAGT5 7 F47 ACGTAGGAGTTTTG 94 51 R1 CACGCCGATTAACA 4 P1AGTCGCGGCGTTTATTGGTTTTCGGAGT 5 8 F48 CGTAGGAGTTTTGG 93 52 R1CACGCCGATTAACA 4 P1 AGTCGCGGCGTTTATTGGTTTTCGGAGT 5 9 F49 GTAGGAGTTTTGGT92 53 R1 CACGCCGATTAACA 4 P1 AGTCGCGGCGTTTATTGGTTTTCGGAGT 5 0 F50TAGGAGTTTTGGTT 91 54 R1 CACGCCGATTAACA 4 P1 AGTCGCGGCGTTTATTGGTTTTCGGAGT5 1 F51 AGGAGTTTTGGTTT 90 55 R1 CACGCCGATTAACA 4 P1AGTCGCGGCGTTTATTGGTTTTCGGAGT 5 2 F52 GGAGTTTTGGTTTG 89 56 R1CACGCCGATTAACA 4 P1 AGTCGCGGCGTTTATTGGTTTTCGGAGT 5 3 F53 GAGTTTTGGTTTGT88 57 R1 CACGCCGATTAACA 4 P1 AGTCGCGGCGTTTATTGGTTTTCGGAGT 5 4 F54AGTTTTGGTTTGTC 87 58 R1 CACGCCGATTAACA 4 P1 AGTCGCGGCGTTTATTGGTTTTCGGAGT5 5 F55 GTTTTGGTTTGTCG 86 59 R1 CACGCCGATTAACA 4 P1AGTCGCGGCGTTTATTGGTTTTCGGAGT 5 6 F56 TTTTGGTTTGTCGG 85 60 R1CACGCCGATTAACA 4 P1 AGTCGCGGCGTTTATTGGTTTTCGGAGT 5 7 F57 TTTGGTTTGTCGGT84 61 R1 CACGCCGATTAACA 4 P1 AGTCGCGGCGTTTATTGGTTTTCGGAGT 5 8 F58TTGGTTTGTCGGTG 83 62 R1 CACGCCGATTAACA 4 P1 AGTCGCGGCGTTTATTGGTTTTCGGAGT5 9 F59 TGGTTTGTCGGTGA 82 63 R1 CACGCCGATTAACA 4 P1AGTCGCGGCGTTTATTGGTTTTCGGAGT 5 0 F60 GGTTTGTCGGTGAG 81 64 R1CACGCCGATTAACA 4 P1 AGTCGCGGCGTTTATTGGTTTTCGGAGT 5 1 F61 GTTTGTCGGTGAGT80 65 R1 CACGCCGATTAACA 4 P1 AGTCGCGGCGTTTATTGGTTTTCGGAGT 5 2 F62TTTGTCGGTGAGTA 110 66 R2 AATAAACCCGAAAA 67 P2 CGGCGTGTAATTTTGTAGGAATTT68 3 F63 TTGTCGGTGAGTAG 109 69 R2 AATAAACCCGAAAA 67 P2CGGCGTGTAATTTTGTAGGAATTT 68 4 F64 TGTCGGTGAGTAGA 108 70 R2AATAAACCCGAAAA 67 P2 CGGCGTGTAATTTTGTAGGAATTT 68 5 F65 GTCGGTGAGTAGAG107 71 R2 AATAAACCCGAAAA 67 P2 CGGCGTGTAATTTTGTAGGAATTT 68 6 F66TCGGTGAGTAGAGT 106 72 R2 AATAAACCCGAAAA 67 P2 CGGCGTGTAATTTTGTAGGAATTT68 7 F67 CGGTGAGTAGAGTC 105 73 R2 AATAAACCCGAAAA 67 P2CGGCGTGTAATTTTGTAGGAATTT 68 8 F68 GGTGAGTAGAGTCG 104 74 R2AATAAACCCGAAAA 67 P2 CGGCGTGTAATTTTGTAGGAATTT 68 9 F69 GTGAGTAGAGTCGG103 75 R2 AATAAACCCGAAAA 67 P2 CGGCGTGTAATTTTGTAGGAATTT 68 0 F70TGAGTAGAGTCGGC 102 76 R2 AATAAACCCGAAAA 67 P2 CGGCGTGTAATTTTGTAGGAATTT68 1 F71 GAGTAGAGTCGGCG 101 77 R2 AATAAACCCGAAAA 67 P2CGGCGTGTAATTTTGTAGGAATTT 68 2 F72 AGTAGAGTCGGCGT 100 78 R2AATAAACCCGAAAA 67 P2 CGGCGTGTAATTTTGTAGGAATTT 68 3 F73 GTAGAGTCGGCGTA 9979 R2 AATAAACCCGAAAA 67 P2 CGGCGTGTAATTTTGTAGGAATTT 68 4 F74TAGAGTCGGCGTAG 98 80 R2 AATAAACCCGAAAA 67 P2 CGGCGTGTAATTTTGTAGGAATTT 685 F75 AGAGTCGGCGTAGT 97 81 R2 AATAAACCCGAAAA 67 P2CGGCGTGTAATTTTGTAGGAATTT 68 6 F76 GAGTCGGCGTAGTT 96 82 R2 AATAAACCCGAAAA67 P2 CGGCGTGTAATTTTGTAGGAATTT 68 7 F77 AGTCGGCGTAGTTA 95 83 R2AATAAACCCGAAAA 67 P2 CGGCGTGTAATTTTGTAGGAATTT 68 8 F78 GTCGGCGTAGTTAT 9484 R2 AATAAACCCGAAAA 67 P2 CGGCGTGTAATTTTGTAGGAATTT 68 9 F79TCGGCGTAGTTATA 93 85 R2 AATAAACCCGAAAA 67 P2 CGGCGTGTAATTTTGTAGGAATTT 680 F80 CGGCGTAGTTATAG 92 86 R2 AATAAACCCGAAAA 67 P2CGGCGTGTAATTTTGTAGGAATTT 68 1 F81 GGCGTAGTTATAGC 91 87 R2 AATAAACCCGAAAA67 P2 CGGCGTGTAATTTTGTAGGAATTT 68 2 F82 GCGTAGTTATAGCG 90 88 R2AATAAACCCGAAAA 67 P2 CGGCGTGTAATTTTGTAGGAATTT 68 3 F83 CGTAGTTATAGCGC 8989 R2 AATAAACCCGAAAA 67 P2 CGGCGTGTAATTTTGTAGGAATTT 68 4 F84GTAGTTATAGCGCG 88 90 R2 AATAAACCCGAAAA 67 P2 CGGCGTGTAATTTTGTAGGAATTT 685 F85 TAGTTATAGCGCGG 87 91 R2 AATAAACCCGAAAA 67 P2CGGCGTGTAATTTTGTAGGAATTT 68 6 F86 AGTTATAGCGCGGA 86 92 R2 AATAAACCCGAAAA67 P2 CGGCGTGTAATTTTGTAGGAATTT 68 7 F87 GTTATAGCGCGGAG 85 93 R2AATAAACCCGAAAA 67 P2 CGGCGTGTAATTTTGTAGGAATTT 68 8 F88 TTATAGCGCGGAGT 8494 R2 AATAAACCCGAAAA 67 P2 CGGCGTGTAATTTTGTAGGAATTT 68 9 F89TATAGCGCGGAGTC 83 95 R2 AATAAACCCGAAAA 67 P2 CGGCGTGTAATTTTGTAGGAATTT 680 F90 ATAGCGCGGAGTCG 82 96 R2 AATAAACCCGAAAA 67 P2CGGCGTGTAATTTTGTAGGAATTT 68 1 F91 TAGCGCGGAGTCGC 81 97 R2 AATAAACCCGAAAA67 P2 CGGCGTGTAATTTTGTAGGAATTT 68 2 F92 AGCGCGGAGTCGCG 80 98 R2AATAAACCCGAAAA 67 P2 CGGCGTGTAATTTTGTAGGAATTT 68 3 F93 GCGCGGAGTCGCGG130 99 R3 CTCCGAACTCCCCT 100 P3 CGTTTTTTTTTTTTAGTCGTTT 101 4 F94CGCGGAGTCGCGGC 129 102 R3 CTCCGAACTCCCCT 100 P3 CGTTTTTTTTTTTTAGTCGTTT101 5 F95 GCGGAGTCGCGGCG 128 103 R3 CTCCGAACTCCCCT 100 P3CGTTTTTTTTTTTTAGTCGTTT 101 6 F96 CGGAGTCGCGGCGT 127 104 R3CTCCGAACTCCCCT 100 P3 CGTTTTTTTTTTTTAGTCGTTT 101 7 F97 GGAGTCGCGGCGTT126 105 R3 CTCCGAACTCCCCT 100 P3 CGTTTTTTTTTTTTAGTCGTTT 101 8 F98GAGTCGCGGCGTTT 125 106 R3 CTCCGAACTCCCCT 100 P3 CGTTTTTTTTTTTTAGTCGTTT101 9 F99 AGTCGCGGCGTTTA 124 107 R3 CTCCGAACTCCCCT 100 P3CGTTTTTTTTTTTTAGTCGTTT 101 00 F100 GTCGCGGCGTTTAT 123 108 R3CTCCGAACTCCCCT 100 P3 CGTTTTTTTTTTTTAGTCGTTT 101 01 F101 TCGCGGCGTTTATT122 109 R3 CTCCGAACTCCCCT 100 P3 CGTTTTTTTTTTTTAGTCGTTT 101 02 F102CGCGGCGTTTATTG 121 110 R3 CTCCGAACTCCCCT 100 P3 CGTTTTTTTTTTTTAGTCGTTT101 03 F103 GCGGCGTTTATTGG 120 111 R3 CTCCGAACTCCCCT 100 P3CGTTTTTTTTTTTTAGTCGTTT 101 04 F104 CGGCGTTTATTGGT 119 112 R3CTCCGAACTCCCCT 100 P3 CGTTTTTTTTTTTTAGTCGTTT 101 05 F105 GGCGTTTATTGGTT118 113 R3 CTCCGAACTCCCCT 100 P3 CGTTTTTTTTTTTTAGTCGTTT 101 06 F106GCGTTTATTGGTTT 117 114 R3 CTCCGAACTCCCCT 100 P3 CGTTTTTTTTTTTTAGTCGTTT101 07 F107 CGTTTATTGGTTTT 116 115 R3 CTCCGAACTCCCCT 100 P3CGTTTTTTTTTTTTAGTCGTTT 101 08 F108 GTTTATTGGTTTTC 115 116 R3CTCCGAACTCCCCT 100 P3 CGTTTTTTTTTTTTAGTCGTTT 101 09 F109 TTTATTGGTTTTCG114 117 R3 CTCCGAACTCCCCT 100 P3 CGTTTTTTTTTTTTAGTCGTTT 101 10 F110TTATTGGTTTTCGG 113 118 R3 CTCCGAACTCCCCT 100 P3 CGTTTTTTTTTTTTAGTCGTTT101 11 F111 TATTGGTTTTCGGA 112 119 R3 CTCCGAACTCCCCT 100 P3CGTTTTTTTTTTTTAGTCGTTT 101 12 F112 ATTGGTTTTCGGAG 111 120 R3CTCCGAACTCCCCT 100 P3 CGTTTTTTTTTTTTAGTCGTTT 101 13 F113 TTGGTTTTCGGAGT110 121 R3 CTCCGAACTCCCCT 100 P3 CGTTTTTTTTTTTTAGTCGTTT 101 14 F114TGGTTTTCGGAGTT 109 122 R3 CTCCGAACTCCCCT 100 P3 CGTTTTTTTTTTTTAGTCGTTT101 15 F115 GGTTTTCGGAGTTG 108 123 R3 CTCCGAACTCCCCT 100 P3CGTTTTTTTTTTTTAGTCGTTT 101 16 F116 GTTTTCGGAGTTGT 107 124 R3CTCCGAACTCCCCT 100 P3 CGTTTTTTTTTTTTAGTCGTTT 101 17 F117 TTTTCGGAGTTGTT106 125 R3 CTCCGAACTCCCCT 100 P3 CGTTTTTTTTTTTTAGTCGTTT 101 18 F118TTTCGGAGTTGTTA 105 126 R3 CTCCGAACTCCCCT 100 P3 CGTTTTTTTTTTTTAGTCGTTT101 19 F119 TTCGGAGTTGTTAA 104 127 R3 CTCCGAACTCCCCT 100 P3CGTTTTTTTTTTTTAGTCGTTT 101 20 F120 TCGGAGTTGTTAAT 103 128 R3CTCCGAACTCCCCT 100 P3 CGTTTTTTTTTTTTAGTCGTTT 101 21 F121 CGGAGTTGTTAATC102 129 R3 CTCCGAACTCCCCT 100 P3 CGTTTTTTTTTTTTAGTCGTTT 101 22 F122GGAGTTGTTAATCG 101 130 R3 CTCCGAACTCCCCT 100 P3 CGTTTTTTTTTTTTAGTCGTTT101 23 F123 GAGTTGTTAATCGG 100 131 R3 CTCCGAACTCCCCT 100 P3CGTTTTTTTTTTTTAGTCGTTT 101 24 F124 AGTTGTTAATCGGC 99 132 R3CTCCGAACTCCCCT 100 P3 CGTTTTTTTTTTTTAGTCGTTT 101 25 F125 GTTGTTAATCGGCG98 133 R3 CTCCGAACTCCCCT 100 P3 CGTTTTTTTTTTTTAGTCGTTT 101 26 F126TTGTTAATCGGCGT 97 134 R3 CTCCGAACTCCCCT 100 P3 CGTTTTTTTTTTTTAGTCGTTT101 27 F127 TGTTAATCGGCGTG 96 R3 CTCCGAACTCCCCT 100 P3CGTTTTTTTTTTTTAGTCGTTT 101 28 F128 GTTAATCGGCGTGT 95 136 R3CTCCGAACTCCCCT 100 P3 CGTTTTTTTTTTTTAGTCGTTT 101 29 F129 TTAATCGGCGTGTA94 137 R3 CTCCGAACTCCCCT 100 P3 CGTTTTTTTTTTTTAGTCGTTT 101 30 F130TAATCGGCGTGTAA 93 138 R3 CTCCGAACTCCCCT 100 P3 CGTTTTTTTTTTTTAGTCGTTT101 31 F131 AATCGGCGTGTAAT 92 139 R3 CTCCGAACTCCCCT 100 P3CGTTTTTTTTTTTTAGTCGTTT 101 32 F132 ATCGGCGTGTAATT 91 140 R3CTCCGAACTCCCCT 100 P3 CGTTTTTTTTTTTTAGTCGTTT 101 33 F133 TCGGCGTGTAATTT90 141 R3 CTCCGAACTCCCCT 100 P3 CGTTTTTTTTTTTTAGTCGTTT 101 34 F134CGGCGTGTAATTTT 89 142 R3 CTCCGAACTCCCCT 100 P3 CGTTTTTTTTTTTTAGTCGTTT101 35 F135 GGCGTGTAATTTTG 88 143 R3 CTCCGAACTCCCCT 100 P3CGTTTTTTTTTTTTAGTCGTTT 101 36 F136 GCGTGTAATTTTGT 87 144 R3CTCCGAACTCCCCT 100 P3 CGTTTTTTTTTTTTAGTCGTTT 101 37 F137 CGTGTAATTTTGTA86 145 R3 CTCCGAACTCCCCT 100 P3 CGTTTTTTTTTTTTAGTCGTTT 101 38 F138GTGTAATTTTGTAG 85 146 R3 CTCCGAACTCCCCT 100 P3 CGTTTTTTTTTTTTAGTCGTTT101 39 F139 TGTAATTTTGTAGG 84 147 R3 CTCCGAACTCCCCT 100 P3CGTTTTTTTTTTTTAGTCGTTT 101 40 F140 GTAATTTTGTAGGA 83 148 R3CTCCGAACTCCCCT 100 P3 CGTTTTTTTTTTTTAGTCGTTT 101 41 F141 TAATTTTGTAGGAA82 149 R3 CTCCGAACTCCCCT 100 P3 CGTTTTTTTTTTTTAGTCGTTT 101 42 F142AATTTTGTAGGAAT 81 150 R3 CTCCGAACTCCCCT 100 P3 CGTTTTTTTTTTTTAGTCGTTT101 43 F143 ATTTTGTAGGAATT 80 151 R3 CTCCGAACTCCCCT 100 P3CGTTTTTTTTTTTTAGTCGTTT 101 44 F144 TTTTGTAGGAATTT 140 152 R4CGAATCCTCCTCCT 153 P4 TTAGAGGAAAAGAAGAGGAGGAGA 154 45 F145TTTGTAGGAATTTT 139 155 R4 CGAATCCTCCTCCT 153 P4 TTAGAGGAAAAGAAGAGGAGGAGA154 46 F146 TTGTAGGAATTTTT 138 156 R4 CGAATCCTCCTCCT 153 P4TTAGAGGAAAAGAAGAGGAGGAGA 154 47 F147 TGTAGGAATTTTTT 137 157 R4CGAATCCTCCTCCT 153 P4 TTAGAGGAAAAGAAGAGGAGGAGA 154 48 F148GTAGGAATTTTTTT 136 158 R4 CGAATCCTCCTCCT 153 P4 TTAGAGGAAAAGAAGAGGAGGAGA154 49 F149 TAGGAATTTTTTTC 135 159 R4 CGAATCCTCCTCCT 153 P4TTAGAGGAAAAGAAGAGGAGGAGA 154 50 F150 AGGAATTTTTTTCG 134 160 R4CGAATCCTCCTCCT 153 P4 TTAGAGGAAAAGAAGAGGAGGAGA 154 51 F151GGAATTTTTTTCGG 133 161 R4 CGAATCCTCCTCCT 153 P4 TTAGAGGAAAAGAAGAGGAGGAGA154 52 F152 GAATTTTTTTCGGG 132 162 R4 CGAATCCTCCTCCT 153 P4TTAGAGGAAAAGAAGAGGAGGAGA 154 53 F153 AATTTTTTTCGGGT 131 163 R4CGAATCCTCCTCCT 153 P4 TTAGAGGAAAAGAAGAGGAGGAGA 154 54 F154ATTTTTTTCGGGTT 130 164 R4 CGAATCCTCCTCCT 153 P4 TTAGAGGAAAAGAAGAGGAGGAGA154 55 F155 TTTTTTTCGGGTTT 129 165 R4 CGAATCCTCCTCCT 153 P4TTAGAGGAAAAGAAGAGGAGGAGA 154 56 F156 TTTTTTCGGGTTTA 128 166 R4CGAATCCTCCTCCT 153 P4 TTAGAGGAAAAGAAGAGGAGGAGA 154 57 F157TTTTTCGGGTTTAT 127 167 R4 CGAATCCTCCTCCT 153 P4 TTAGAGGAAAAGAAGAGGAGGAGA154 58 F158 TTTTCGGGTTTATT 126 168 R4 CGAATCCTCCTCCT 153 P4TTAGAGGAAAAGAAGAGGAGGAGA 154 59 F159 TTTCGGGTTTATTT 125 169 R4CGAATCCTCCTCCT 153 P4 TTAGAGGAAAAGAAGAGGAGGAGA 154 60 F160TTCGGGTTTATTTG 124 170 R4 CGAATCCTCCTCCT 153 P4 TTAGAGGAAAAGAAGAGGAGGAGA154 61 F161 TCGGGTTTATTTGG 123 171 R4 CGAATCCTCCTCCT 153 P4TTAGAGGAAAAGAAGAGGAGGAGA 154 62 F162 CGGGTTTATTTGGG 122 172 R4CGAATCCTCCTCCT 153 P4 TTAGAGGAAAAGAAGAGGAGGAGA 154 63 F163GGGTTTATTTGGGA 121 173 R4 CGAATCCTCCTCCT 153 P4 TTAGAGGAAAAGAAGAGGAGGAGA154 64 F164 GGTTTATTTGGGAG 120 174 R4 CGAATCCTCCTCCT 153 P4TTAGAGGAAAAGAAGAGGAGGAGA 154 65 F165 GTTTATTTGGGAGT 119 175 R4CGAATCCTCCTCCT 153 P4 TTAGAGGAAAAGAAGAGGAGGAGA 154 66 F166TTTATTTGGGAGTT 118 176 R4 CGAATCCTCCTCCT 153 P4 TTAGAGGAAAAGAAGAGGAGGAGA154 67 F167 TTATTTGGGAGTTA 117 177 R4 CGAATCCTCCTCCT 153 P4TTAGAGGAAAAGAAGAGGAGGAGA 154 68 F168 TATTTGGGAGTTAT 116 178 R4CGAATCCTCCTCCT 153 P4 TTAGAGGAAAAGAAGAGGAGGAGA 154 69 F169ATTTGGGAGTTATA 115 179 R4 CGAATCCTCCTCCT 153 P4 TTAGAGGAAAAGAAGAGGAGGAGA154 70 F170 TTTGGGAGTTATAT 114 180 R4 CGAATCCTCCTCCT 153 P4TTAGAGGAAAAGAAGAGGAGGAGA 154 71 F171 TTGGGAGTTATATT 113 181 R4CGAATCCTCCTCCT 153 P4 TTAGAGGAAAAGAAGAGGAGGAGA 154 72 F172TGGGAGTTATATTG 112 182 R4 CGAATCCTCCTCCT 153 P4 TTAGAGGAAAAGAAGAGGAGGAGA154 73 F173 GGGAGTTATATTGT 111 183 R4 CGAATCCTCCTCCT 153 P4TTAGAGGAAAAGAAGAGGAGGAGA 154 74 F174 GGAGTTATATTGTC 110 184 R4CGAATCCTCCTCCT 153 P4 TTAGAGGAAAAGAAGAGGAGGAGA 154 75 F175GAGTTATATTGTCG 109 185 R4 CGAATCCTCCTCCT 153 P4 TTAGAGGAAAAGAAGAGGAGGAGA154 76 F176 AGTTATATTGTCGT 108 186 R4 CGAATCCTCCTCCT 153 P4TTAGAGGAAAAGAAGAGGAGGAGA 154 77 F177 GTTATATTGTCGTT 107 187 R4CGAATCCTCCTCCT 153 P4 TTAGAGGAAAAGAAGAGGAGGAGA 154 78 F178TTATATTGTCGTTT 106 188 R4 CGAATCCTCCTCCT 153 P4 TTAGAGGAAAAGAAGAGGAGGAGA154 79 F179 TATATTGTCGTTTT 105 189 R4 CGAATCCTCCTCCT 153 P4TTAGAGGAAAAGAAGAGGAGGAGA 154 80 F180 ATATTGTCGTTTTT 104 190 R4CGAATCCTCCTCCT 153 P4 TTAGAGGAAAAGAAGAGGAGGAGA 154 81 F181TATTGTCGTTTTTT 103 191 R4 CGAATCCTCCTCCT 153 P4 TTAGAGGAAAAGAAGAGGAGGAGA154 82 F182 ATTGTCGTTTTTTT 102 192 R4 CGAATCCTCCTCCT 153 P4TTAGAGGAAAAGAAGAGGAGGAGA 154 83 F183 TTGTCGTTTTTTTT 101 193 R4CGAATCCTCCTCCT 153 P4 TTAGAGGAAAAGAAGAGGAGGAGA 154 84 F184TGTCGTTTTTTTTT 100 194 R4 CGAATCCTCCTCCT 153 P4 TTAGAGGAAAAGAAGAGGAGGAGA154 85 F185 GTCGTTTTTTTTTT 99 195 R4 CGAATCCTCCTCCT 153 P4TTAGAGGAAAAGAAGAGGAGGAGA 154 86 F186 TCGTTTTTTTTTTT 98 196 R4CGAATCCTCCTCCT 153 P4 TTAGAGGAAAAGAAGAGGAGGAGA 154 87 F187CGTTTTTTTTTTTT 97 197 R4 CGAATCCTCCTCCT 153 P4 TTAGAGGAAAAGAAGAGGAGGAGA154 88 F188 GTTTTTTTTTTTTA 96 198 R4 CGAATCCTCCTCCT 153 P4TTAGAGGAAAAGAAGAGGAGGAGA 154 89 F189 TTTTTTTTTTTTAG 95 199 R4CGAATCCTCCTCCT 153 P4 TTAGAGGAAAAGAAGAGGAGGAGA 154 90 F190TTTTTTTTTTTAGT 94 200 R4 CGAATCCTCCTCCT 153 P4 TTAGAGGAAAAGAAGAGGAGGAGA154 91 F191 TTTTTTTTTTAGTC 93 201 R4 CGAATCCTCCTCCT 153 P4TTAGAGGAAAAGAAGAGGAGGAGA 154 92 F192 TTTTTTTTTAGTCG 92 202 R4CGAATCCTCCTCCT 153 P4 TTAGAGGAAAAGAAGAGGAGGAGA 154 93 F193TTTTTTTTAGTCGT 91 203 R4 CGAATCCTCCTCCT 153 P4 TTAGAGGAAAAGAAGAGGAGGAGA154 94 F194 TTTTTTTAGTCGTT 90 204 R4 CGAATCCTCCTCCT 153 P4TTAGAGGAAAAGAAGAGGAGGAGA 154 95 F195 TTTTTTAGTCGTTT 89 205 R4CGAATCCTCCTCCT 153 P4 TTAGAGGAAAAGAAGAGGAGGAGA 154 96 F196TTTTTAGTCGTTTA 88 206 R4 CGAATCCTCCTCCT 153 P4 TTAGAGGAAAAGAAGAGGAGGAGA154 97 F197 TTTTAGTCGTTTAG 87 207 R4 CGAATCCTCCTCCT 153 P4TTAGAGGAAAAGAAGAGGAGGAGA 154 98 F198 TTTAGTCGTTTAGG 86 208 R4CGAATCCTCCTCCT 153 P4 TTAGAGGAAAAGAAGAGGAGGAGA 154 99 F199TTAGTCGTTTAGGG 85 209 R4 CGAATCCTCCTCCT 153 P4 TTAGAGGAAAAGAAGAGGAGGAGA154 00 F200 TAGTCGTTTAGGGG 84 210 R4 CGAATCCTCCTCCT 153 P4TTAGAGGAAAAGAAGAGGAGGAGA 154 01 F201 AGTCGTTTAGGGGA 83 211 R4CGAATCCTCCTCCT 153 P4 TTAGAGGAAAAGAAGAGGAGGAGA 154 02 F202GTCGTTTAGGGGAG 82 212 R4 CGAATCCTCCTCCT 153 P4 TTAGAGGAAAAGAAGAGGAGGAGA154 03 F203 TCGTTTAGGGGAGT 81 213 R4 CGAATCCTCCTCCT 153 P4TTAGAGGAAAAGAAGAGGAGGAGA 154 04 F204 CGTTTAGGGGAGTT 80 214 R4CGAATCCTCCTCCT 153 P4 TTAGAGGAAAAGAAGAGGAGGAGA 154 05 F205GTTTAGGGGAGTTC 140 215 R5 CAAACGAAACCACT 216 P5 AGGGGCGTAGTCGCGGAGTT 21706 F206 TTTAGGGGAGTTCG 139 218 R5 CAAACGAAACCACT 216 P5AGGGGCGTAGTCGCGGAGTT 217 07 F207 TTAGGGGAGTTCGG 138 219 R5CAAACGAAACCACT 216 P5 AGGGGCGTAGTCGCGGAGTT 217 08 F208 TAGGGGAGTTCGGA137 220 R5 CAAACGAAACCACT 216 P5 AGGGGCGTAGTCGCGGAGTT 217 09 F209AGGGGAGTTCGGAG 136 221 R5 CAAACGAAACCACT 216 P5 AGGGGCGTAGTCGCGGAGTT 21710 F210 GGGGAGTTCGGAGA 135 222 R5 CAAACGAAACCACT 216 P5AGGGGCGTAGTCGCGGAGTT 217 11 F211 GGGAGTTCGGAGAA 134 223 R5CAAACGAAACCACT 216 P5 AGGGGCGTAGTCGCGGAGTT 217 12 F212 GGAGTTCGGAGAAG133 224 R5 CAAACGAAACCACT 216 P5 AGGGGCGTAGTCGCGGAGTT 217 13 F213GAGTTCGGAGAAGT 132 225 R5 CAAACGAAACCACT 216 P5 AGGGGCGTAGTCGCGGAGTT 21714 F214 AGTTCGGAGAAGTA 131 226 R5 CAAACGAAACCACT 216 P5AGGGGCGTAGTCGCGGAGTT 217 15 F215 GTTCGGAGAAGTAG 130 227 R5CAAACGAAACCACT 216 P5 AGGGGCGTAGTCGCGGAGTT 217 16 F216 TTCGGAGAAGTAGG129 228 R5 CAAACGAAACCACT 216 P5 AGGGGCGTAGTCGCGGAGTT 217 17 F217TCGGAGAAGTAGGT 128 229 R5 CAAACGAAACCACT 216 P5 AGGGGCGTAGTCGCGGAGTT 21718 F218 CGGAGAAGTAGGTT 127 230 R5 CAAACGAAACCACT 216 P5AGGGGCGTAGTCGCGGAGTT 217 19 F219 GGAGAAGTAGGTTT 126 231 R5CAAACGAAACCACT 216 P5 AGGGGCGTAGTCGCGGAGTT 217 20 F220 GAGAAGTAGGTTTA125 232 R5 CAAACGAAACCACT 216 P5 AGGGGCGTAGTCGCGGAGTT 217 21 F221AGAAGTAGGTTTAG 124 233 R5 CAAACGAAACCACT 216 P5 AGGGGCGTAGTCGCGGAGTT 21722 F222 GAAGTAGGTTTAGG 123 234 R5 CAAACGAAACCACT 216 P5AGGGGCGTAGTCGCGGAGTT 217 23 F223 AAGTAGGTTTAGGA 122 235 R5CAAACGAAACCACT 216 P5 AGGGGCGTAGTCGCGGAGTT 217 24 F224 AGTAGGTTTAGGAG121 236 R5 CAAACGAAACCACT 216 P5 AGGGGCGTAGTCGCGGAGTT 217 25 F225GTAGGTTTAGGAGG 120 237 R5 CAAACGAAACCACT 216 P5 AGGGGCGTAGTCGCGGAGTT 21726 F226 TAGGTTTAGGAGGG 119 238 R5 CAAACGAAACCACT 216 P5AGGGGCGTAGTCGCGGAGTT 217 27 F227 AGGTTTAGGAGGGA 118 239 R5CAAACGAAACCACT 216 P5 AGGGGCGTAGTCGCGGAGTT 217 28 F228 GGTTTAGGAGGGAG117 240 R5 CAAACGAAACCACT 216 P5 AGGGGCGTAGTCGCGGAGTT 217 29 F229GTTTAGGAGGGAGG 116 241 R5 CAAACGAAACCACT 216 P5 AGGGGCGTAGTCGCGGAGTT 21730 F230 TTTAGGAGGGAGGG 115 242 R5 CAAACGAAACCACT 216 P5AGGGGCGTAGTCGCGGAGTT 217 31 F231 TTAGGAGGGAGGGA 114 243 R5CAAACGAAACCACT 216 P5 AGGGGCGTAGTCGCGGAGTT 217 32 F232 TAGGAGGGAGGGAG113 244 R5 CAAACGAAACCACT 216 P5 AGGGGCGTAGTCGCGGAGTT 217 33 F233AGGAGGGAGGGAGT 112 245 R5 CAAACGAAACCACT 216 P5 AGGGGCGTAGTCGCGGAGTT 21734 F234 GGAGGGAGGGAGTT 111 246 R5 CAAACGAAACCACT 216 P5AGGGGCGTAGTCGCGGAGTT 217 35 F235 GAGGGAGGGAGTTA 110 247 R5CAAACGAAACCACT 216 P5 AGGGGCGTAGTCGCGGAGTT 217 36 F236 AGGGAGGGAGTTAG109 248 R5 CAAACGAAACCACT 216 P5 AGGGGCGTAGTCGCGGAGTT 217 37 F237GGGAGGGAGTTAGA 108 249 R5 CAAACGAAACCACT 216 P5 AGGGGCGTAGTCGCGGAGTT 21738 F238 GGAGGGAGTTAGAG 107 250 R5 CAAACGAAACCACT 216 P5AGGGGCGTAGTCGCGGAGTT 217 39 F239 GAGGGAGTTAGAGG 106 251 R5CAAACGAAACCACT 216 P5 AGGGGCGTAGTCGCGGAGTT 217 40 F240 AGGGAGTTAGAGGA105 252 R5 CAAACGAAACCACT 216 P5 AGGGGCGTAGTCGCGGAGTT 217 41 F241GGGAGTTAGAGGAA 104 253 R5 CAAACGAAACCACT 216 P5 AGGGGCGTAGTCGCGGAGTT 21742 F242 GGAGTTAGAGGAAA 103 254 R5 CAAACGAAACCACT 216 P5AGGGGCGTAGTCGCGGAGTT 217 43 F243 GAGTTAGAGGAAAA 102 255 R5CAAACGAAACCACT 216 P5 AGGGGCGTAGTCGCGGAGTT 217 44 F244 AGTTAGAGGAAAAG101 256 R5 CAAACGAAACCACT 216 P5 AGGGGCGTAGTCGCGGAGTT 217 45 F245GTTAGAGGAAAAGA 100 257 R5 CAAACGAAACCACT 216 P5 AGGGGCGTAGTCGCGGAGTT 21746 F246 TTAGAGGAAAAGAA 99 258 R5 CAAACGAAACCACT 216 P5AGGGGCGTAGTCGCGGAGTT 217 47 F247 TAGAGGAAAAGAAG 98 259 R5 CAAACGAAACCACT216 P5 AGGGGCGTAGTCGCGGAGTT 217 48 F248 AGAGGAAAAGAAGA 97 260 R5CAAACGAAACCACT 216 P5 AGGGGCGTAGTCGCGGAGTT 217 49 F249 GAGGAAAAGAAGAG 96261 R5 CAAACGAAACCACT 216 P5 AGGGGCGTAGTCGCGGAGTT 217 50 F250AGGAAAAGAAGAGG 95 262 R5 CAAACGAAACCACT 216 P5 AGGGGCGTAGTCGCGGAGTT 21751 F251 GGAAAAGAAGAGGA 94 263 R5 CAAACGAAACCACT 216 P5AGGGGCGTAGTCGCGGAGTT 217 52 F252 GAAAAGAAGAGGAG 93 264 R5 CAAACGAAACCACT216 P5 AGGGGCGTAGTCGCGGAGTT 217 53 F253 AAAAGAAGAGGAGG 92 265 R5CAAACGAAACCACT 216 P5 AGGGGCGTAGTCGCGGAGTT 217 54 F254 AAAGAAGAGGAGGA 91266 R5 CAAACGAAACCACT 216 P5 AGGGGCGTAGTCGCGGAGTT 217 55 F255AAGAAGAGGAGGAG 90 267 R5 CAAACGAAACCACT 216 P5 AGGGGCGTAGTCGCGGAGTT 21756 F256 AGAAGAGGAGGAGA 89 268 R5 CAAACGAAACCACT 216 P5AGGGGCGTAGTCGCGGAGTT 217 57 F257 GAAGAGGAGGAGAA 88 269 R5 CAAACGAAACCACT216 P5 AGGGGCGTAGTCGCGGAGTT 217 58 F258 AAGAGGAGGAGAAG 87 270 R5CAAACGAAACCACT 216 P5 AGGGGCGTAGTCGCGGAGTT 217 59 F259 AGAGGAGGAGAAGG 86271 R5 CAAACGAAACCACT 216 P5 AGGGGCGTAGTCGCGGAGTT 217 60 F260GAGGAGGAGAAGGA 85 272 R5 CAAACGAAACCACT 216 P5 AGGGGCGTAGTCGCGGAGTT 21761 F261 AGGAGGAGAAGGAG 84 273 R5 CAAACGAAACCACT 216 P5AGGGGCGTAGTCGCGGAGTT 217 62 F262 GGAGGAGAAGGAGG 83 274 R5 CAAACGAAACCACT216 P5 AGGGGCGTAGTCGCGGAGTT 217 63 F263 GAGGAGAAGGAGGA 82 275 R5CAAACGAAACCACT 216 P5 AGGGGCGTAGTCGCGGAGTT 217 64 F264 AGGAGAAGGAGGAG 81276 R5 CAAACGAAACCACT 216 P5 AGGGGCGTAGTCGCGGAGTT 217 65 F265GGAGAAGGAGGAGG 80 277 R5 CAAACGAAACCACT 216 P5 AGGGGCGTAGTCGCGGAGTT 21766 F266 GAGAAGGAGGAGGA 140 278 R6 ACGACGAAAACGCG 279 P6CGGAGTTTTAGTCGCGCGGATCG 280 67 F267 AGAAGGAGGAGGAT 139 281 R6ACGACGAAAACGCG 279 P6 CGGAGTTTTAGTCGCGCGGATCG 280 68 F268 GAAGGAGGAGGATT138 282 R6 ACGACGAAAACGCG 279 P6 CGGAGTTTTAGTCGCGCGGATCG 280 69 F269AAGGAGGAGGATTC 137 283 R6 ACGACGAAAACGCG 279 P6 CGGAGTTTTAGTCGCGCGGATCG280 70 F270 AGGAGGAGGATTCG 136 284 R6 ACGACGAAAACGCG 279 P6CGGAGTTTTAGTCGCGCGGATCG 280 71 F271 GGAGGAGGATTCGG 135 285 R6ACGACGAAAACGCG 279 P6 CGGAGTTTTAGTCGCGCGGATCG 280 72 F272 GAGGAGGATTCGGG134 286 R6 ACGACGAAAACGCG 279 P6 CGGAGTTTTAGTCGCGCGGATCG 280 73 F273AGGAGGATTCGGGG 133 287 R6 ACGACGAAAACGCG 279 P6 CGGAGTTTTAGTCGCGCGGATCG280 74 F274 GGAGGATTCGGGGA 132 288 R6 ACGACGAAAACGCG 279 P6CGGAGTTTTAGTCGCGCGGATCG 280 75 F275 GAGGATTCGGGGAG 131 289 R6ACGACGAAAACGCG 279 P6 CGGAGTTTTAGTCGCGCGGATCG 280 76 F276 AGGATTCGGGGAGG130 290 R6 ACGACGAAAACGCG 279 P6 CGGAGTTTTAGTCGCGCGGATCG 280 77 F277GGATTCGGGGAGGG 129 291 R6 ACGACGAAAACGCG 279 P6 CGGAGTTTTAGTCGCGCGGATCG280 78 F278 GATTCGGGGAGGGA 128 292 R6 ACGACGAAAACGCG 279 P6CGGAGTTTTAGTCGCGCGGATCG 280 79 F279 ATTCGGGGAGGGAG 127 293 R6ACGACGAAAACGCG 279 P6 CGGAGTTTTAGTCGCGCGGATCG 280 80 F280 TTCGGGGAGGGAGG126 294 R6 ACGACGAAAACGCG 279 P6 CGGAGTTTTAGTCGCGCGGATCG 280 81 F281TCGGGGAGGGAGGC 125 295 R6 ACGACGAAAACGCG 279 P6 CGGAGTTTTAGTCGCGCGGATCG280 82 F282 CGGGGAGGGAGGCG 124 296 R6 ACGACGAAAACGCG 279 P6CGGAGTTTTAGTCGCGCGGATCG 280 83 F283 GGGGAGGGAGGCGC 123 297 R6ACGACGAAAACGCG 279 P6 CGGAGTTTTAGTCGCGCGGATCG 280 84 F284 GGGAGGGAGGCGCG122 298 R6 ACGACGAAAACGCG 279 P6 CGGAGTTTTAGTCGCGCGGATCG 280 85 F285GGAGGGAGGCGCGG 121 299 R6 ACGACGAAAACGCG 279 P6 CGGAGTTTTAGTCGCGCGGATCG280 86 F286 GAGGGAGGCGCGGC 120 300 R6 ACGACGAAAACGCG 279 P6CGGAGTTTTAGTCGCGCGGATCG 280 87 F287 AGGGAGGCGCGGCG 119 301 R6ACGACGAAAACGCG 279 P6 CGGAGTTTTAGTCGCGCGGATCG 280 88 F288 GGGAGGCGCGGCGC118 302 R6 ACGACGAAAACGCG 279 P6 CGGAGTTTTAGTCGCGCGGATCG 280 89 F289GGAGGCGCGGCGCG 117 303 R6 ACGACGAAAACGCG 279 P6 CGGAGTTTTAGTCGCGCGGATCG280 90 F290 GAGGCGCGGCGCGG 116 304 R6 ACGACGAAAACGCG 279 P6CGGAGTTTTAGTCGCGCGGATCG 280 91 F291 AGGCGCGGCGCGGG 115 305 R6ACGACGAAAACGCG 279 P6 CGGAGTTTTAGTCGCGCGGATCG 280 92 F292 GGCGCGGCGCGGGA114 306 R6 ACGACGAAAACGCG 279 P6 CGGAGTTTTAGTCGCGCGGATCG 280 93 F293GCGCGGCGCGGGAG 113 279 R6 ACGACGAAAACGCG 307 P6 CGGAGTTTTAGTCGCGCGGATCG280 94 F294 CGCGGCGCGGGAGG 112 308 R6 ACGACGAAAACGCG 279 P6CGGAGTTTTAGTCGCGCGGATCG 280 95 F295 GCGGCGCGGGAGGA 111 309 R6ACGACGAAAACGCG 279 P6 CGGAGTTTTAGTCGCGCGGATCG 280 96 F296 CGGCGCGGGAGGAG110 310 R6 ACGACGAAAACGCG 279 P6 CGGAGTTTTAGTCGCGCGGATCG 280 97 F297GGCGCGGGAGGAGG 109 311 R6 ACGACGAAAACGCG 279 P6 CGGAGTTTTAGTCGCGCGGATCG280 98 F298 GCGCGGGAGGAGGA 108 312 R6 ACGACGAAAACGCG 279 P6CGGAGTTTTAGTCGCGCGGATCG 280 99 F299 CGCGGGAGGAGGAG 107 313 R6ACGACGAAAACGCG 279 P6 CGGAGTTTTAGTCGCGCGGATCG 280 00 F300 GCGGGAGGAGGAGG106 314 R6 ACGACGAAAACGCG 279 P6 CGGAGTTTTAGTCGCGCGGATCG 280 01 F301CGGGAGGAGGAGGG 105 315 R6 ACGACGAAAACGCG 279 P6 CGGAGTTTTAGTCGCGCGGATCG280 02 F302 GGGAGGAGGAGGGG 104 316 R6 ACGACGAAAACGCG 279 P6CGGAGTTTTAGTCGCGCGGATCG 280 03 F303 GGAGGAGGAGGGGC 103 317 R6ACGACGAAAACGCG 279 P6 CGGAGTTTTAGTCGCGCGGATCG 280 04 F304 GAGGAGGAGGGGCG102 318 R6 ACGACGAAAACGCG 279 P6 CGGAGTTTTAGTCGCGCGGATCG 280 05 F305AGGAGGAGGGGCGT 101 319 R6 ACGACGAAAACGCG 279 P6 CGGAGTTTTAGTCGCGCGGATCG280 06 F306 GGAGGAGGGGCGTA 100 320 R6 ACGACGAAAACGCG 279 P6CGGAGTTTTAGTCGCGCGGATCG 280 07 F307 GAGGAGGGGCGTAG 99 321 R6ACGACGAAAACGCG 279 P6 CGGAGTTTTAGTCGCGCGGATCG 280 08 F308 AGGAGGGGCGTAGT98 322 R6 ACGACGAAAACGCG 279 P6 CGGAGTTTTAGTCGCGCGGATCG 280 09 F309GGAGGGGCGTAGTC 97 323 R6 ACGACGAAAACGCG 279 P6 CGGAGTTTTAGTCGCGCGGATCG280 10 F310 GAGGGGCGTAGTCG 96 324 R6 ACGACGAAAACGCG 279 P6CGGAGTTTTAGTCGCGCGGATCG 280 11 F311 AGGGGCGTAGTCGC 95 325 R6ACGACGAAAACGCG 279 P6 CGGAGTTTTAGTCGCGCGGATCG 280 12 F312 GGGGCGTAGTCGCG94 326 R6 ACGACGAAAACGCG 279 P6 CGGAGTTTTAGTCGCGCGGATCG 280 13 F313GGGCGTAGTCGCGG 93 327 R6 ACGACGAAAACGCG 279 P6 CGGAGTTTTAGTCGCGCGGATCG280 14 F314 GGCGTAGTCGCGGA 92 328 R6 ACGACGAAAACGCG 279 P6CGGAGTTTTAGTCGCGCGGATCG 280 15 F315 GCGTAGTCGCGGAG 91 329 R6ACGACGAAAACGCG 279 P6 CGGAGTTTTAGTCGCGCGGATCG 280 16 F316 CGTAGTCGCGGAGT90 330 R6 ACGACGAAAACGCG 279 P6 CGGAGTTTTAGTCGCGCGGATCG 280 17 F317GTAGTCGCGGAGTT 89 331 R6 ACGACGAAAACGCG 279 P6 CGGAGTTTTAGTCGCGCGGATCG280 18 F318 TAGTCGCGGAGTTA 88 332 R6 ACGACGAAAACGCG 279 P6CGGAGTTTTAGTCGCGCGGATCG 280 19 F319 AGTCGCGGAGTTAG 87 333 R6ACGACGAAAACGCG 279 P6 CGGAGTTTTAGTCGCGCGGATCG 280 20 F320 GTCGCGGAGTTAGT86 334 R6 ACGACGAAAACGCG 279 P6 CGGAGTTTTAGTCGCGCGGATCG 280 21 F321TCGCGGAGTTAGTG 85 335 R6 ACGACGAAAACGCG 279 P6 CGGAGTTTTAGTCGCGCGGATCG280 22 F322 CGCGGAGTTAGTGG 84 336 R6 ACGACGAAAACGCG 279 P6CGGAGTTTTAGTCGCGCGGATCG 280 23 F323 GCGGAGTTAGTGGT 83 337 R6ACGACGAAAACGCG 279 P6 CGGAGTTTTAGTCGCGCGGATCG 280 24 F324 CGGAGTTAGTGGTT82 338 R6 ACGACGAAAACGCG 279 P6 CGGAGTTTTAGTCGCGCGGATCG 280 25 F325GGAGTTAGTGGTTT 81 339 R6 ACGACGAAAACGCG 279 P6 CGGAGTTTTAGTCGCGCGGATCG280 26 F326 GAGTTAGTGGTTTC 80 340 R6 ACGACGAAAACGCG 279 P6CGGAGTTTTAGTCGCGCGGATCG 280 27 F327 AGTTAGTGGTTTCG 130 341 R7AAATAAATTCGCTA 342 P7 TTTGTCGTAGTTTTTTTTTAAGT 343 28 F328 GTTAGTGGTTTCGT129 344 R7 AAATAAATTCGCTA 342 P7 TTTGTCGTAGTTTTTTTTTAAGT 343 29 F329TTAGTGGTTTCGTT 128 345 R7 AAATAAATTCGCTA 342 P7 TTTGTCGTAGTTTTTTTTTAAGT343 30 F330 TAGTGGTTTCGTTT 127 346 R7 AAATAAATTCGCTA 342 P7TTTGTCGTAGTTTTTTTTTAAGT 343 31 F331 AGTGGTTTCGTTTG 126 347 R7AAATAAATTCGCTA 342 P7 TTTGTCGTAGTTTTTTTTTAAGT 343 32 F332 GTGGTTTCGTTTGG125 348 R7 AAATAAATTCGCTA 342 P7 TTTGTCGTAGTTTTTTTTTAAGT 343 33 F333TGGTTTCGTTTGGA 124 349 R7 AAATAAATTCGCTA 342 P7 TTTGTCGTAGTTTTTTTTTAAGT343 34 F334 GGTTTCGTTTGGAC 123 350 R7 AAATAAATTCGCTA 342 P7TTTGTCGTAGTTTTTTTTTAAGT 343 35 F335 GTTTCGTTTGGACG 122 351 R7AAATAAATTCGCTA 342 P7 TTTGTCGTAGTTTTTTTTTAAGT 343 36 F336 TTTCGTTTGGACGC121 352 R7 AAATAAATTCGCTA 342 P7 TTTGTCGTAGTTTTTTTTTAAGT 343 37 F337TTCGTTTGGACGCG 120 353 R7 AAATAAATTCGCTA 342 P7 TTTGTCGTAGTTTTTTTTTAAGT343 38 F338 TCGTTTGGACGCGT 119 354 R7 AAATAAATTCGCTA 342 P7TTTGTCGTAGTTTTTTTTTAAGT 343 39 F339 CGTTTGGACGCGTT 118 355 R7AAATAAATTCGCTA 342 P7 TTTGTCGTAGTTTTTTTTTAAGT 343 40 F340 GTTTGGACGCGTTG117 356 R7 AAATAAATTCGCTA 342 P7 TTTGTCGTAGTTTTTTTTTAAGT 343 41 F341TTTGGACGCGTTGT 116 357 R7 AAATAAATTCGCTA 342 P7 TTTGTCGTAGTTTTTTTTTAAGT343 42 F342 TTGGACGCGTTGTT 115 358 R7 AAATAAATTCGCTA 342 P7TTTGTCGTAGTTTTTTTTTAAGT 343 43 F343 TGGACGCGTTGTTT 114 359 R7AAATAAATTCGCTA 342 P7 TTTGTCGTAGTTTTTTTTTAAGT 343 44 F344 GGACGCGTTGTTTT113 360 R7 AAATAAATTCGCTA 342 P7 TTTGTCGTAGTTTTTTTTTAAGT 343 45 F345GACGCGTTGTTTTT 112 361 R7 AAATAAATTCGCTA 342 P7 TTTGTCGTAGTTTTTTTTTAAGT343 46 F346 ACGCGTTGTTTTTT 111 362 R7 AAATAAATTCGCTA 342 P7TTTGTCGTAGTTTTTTTTTAAGT 343 47 F347 CGCGTTGTTTTTTA 110 363 R7AAATAAATTCGCTA 342 P7 TTTGTCGTAGTTTTTTTTTAAGT 343 48 F348 GCGTTGTTTTTTAG109 364 R7 AAATAAATTCGCTA 342 P7 TTTGTCGTAGTTTTTTTTTAAGT 343 49 F349CGTTGTTTTTTAGA 108 365 R7 AAATAAATTCGCTA 342 P7 TTTGTCGTAGTTTTTTTTTAAGT343 50 F350 GTTGTTTTTTAGAT 107 366 R7 AAATAAATTCGCTA 342 P7TTTGTCGTAGTTTTTTTTTAAGT 343 51 F351 TTGTTTTTTAGATA 106 367 R7AAATAAATTCGCTA 342 P7 TTTGTCGTAGTTTTTTTTTAAGT 343 52 F352 TGTTTTTTAGATAT105 368 R7 AAATAAATTCGCTA 342 P7 TTTGTCGTAGTTTTTTTTTAAGT 343 53 F353GTTTTTTAGATATT 104 369 R7 AAATAAATTCGCTA 342 P7 TTTGTCGTAGTTTTTTTTTAAGT343 54 F354 TTTTTTAGATATTT 103 370 R7 AAATAAATTCGCTA 342 P7TTTGTCGTAGTTTTTTTTTAAGT 343 55 F355 TTTTTAGATATTTT 102 371 R7AAATAAATTCGCTA 342 P7 TTTGTCGTAGTTTTTTTTTAAGT 343 56 F356 TTTTAGATATTTTC101 372 R7 AAATAAATTCGCTA 342 P7 TTTGTCGTAGTTTTTTTTTAAGT 343 57 F357TTTAGATATTTTCG 100 373 R7 AAATAAATTCGCTA 342 P7 TTTGTCGTAGTTTTTTTTTAAGT343 58 F358 TTAGATATTTTCGG 99 374 R7 AAATAAATTCGCTA 342 P7TTTGTCGTAGTTTTTTTTTAAGT 343 59 F359 TAGATATTTTCGGA 98 375 R7AAATAAATTCGCTA 342 P7 TTTGTCGTAGTTTTTTTTTAAGT 343 60 F360 AGATATTTTCGGAG97 376 R7 AAATAAATTCGCTA 342 P7 TTTGTCGTAGTTTTTTTTTAAGT 343 61 F361GATATTTTCGGAGT 96 377 R7 AAATAAATTCGCTA 342 P7 TTTGTCGTAGTTTTTTTTTAAGT343 62 F362 ATATTTTCGGAGTT 95 378 R7 AAATAAATTCGCTA 342 P7TTTGTCGTAGTTTTTTTTTAAGT 343 63 F363 TATTTTCGGAGTTT 94 379 R7AAATAAATTCGCTA 342 P7 TTTGTCGTAGTTTTTTTTTAAGT 343 64 F364 ATTTTCGGAGTTTT93 380 R7 AAATAAATTCGCTA 342 P7 TTTGTCGTAGTTTTTTTTTAAGT 343 65 F365TTTTCGGAGTTTTA 92 381 R7 AAATAAATTCGCTA 342 P7 TTTGTCGTAGTTTTTTTTTAAGT343 66 F366 TTTCGGAGTTTTAG 91 382 R7 AAATAAATTCGCTA 342 P7TTTGTCGTAGTTTTTTTTTAAGT 343 67 F367 TTCGGAGTTTTAGT 90 383 R7AAATAAATTCGCTA 342 P7 TTTGTCGTAGTTTTTTTTTAAGT 343 68 F368 TCGGAGTTTTAGTC89 384 R7 AAATAAATTCGCTA 342 P7 TTTGTCGTAGTTTTTTTTTAAGT 343 69 F369CGGAGTTTTAGTCG 88 385 R7 AAATAAATTCGCTA 342 P7 TTTGTCGTAGTTTTTTTTTAAGT343 70 F370 GGAGTTTTAGTCGC 87 386 R7 AAATAAATTCGCTA 342 P7TTTGTCGTAGTTTTTTTTTAAGT 343 71 F371 GAGTTTTAGTCGCG 86 387 R7AAATAAATTCGCTA 342 P7 TTTGTCGTAGTTTTTTTTTAAGT 343 72 F372 AGTTTTAGTCGCGC85 388 R7 AAATAAATTCGCTA 342 P7 TTTGTCGTAGTTTTTTTTTAAGT 343 73 F373GTTTTAGTCGCGCG 84 389 R7 AAATAAATTCGCTA 342 P7 TTTGTCGTAGTTTTTTTTTAAGT343 74 F374 TTTTAGTCGCGCGG 83 390 R7 AAATAAATTCGCTA 342 P7TTTGTCGTAGTTTTTTTTTAAGT 343 75 F375 TTTAGTCGCGCGGA 82 391 R7AAATAAATTCGCTA 342 P7 TTTGTCGTAGTTTTTTTTTAAGT 343 76 F376 TTAGTCGCGCGGAT81 392 R7 AAATAAATTCGCTA 342 P7 TTTGTCGTAGTTTTTTTTTAAGT 343 77 F377TAGTCGCGCGGATC 80 393 R7 AAATAAATTCGCTA 342 P7 TTTGTCGTAGTTTTTTTTTAAGT343 78 F378 AGTCGCGCGGATCG 130 394 R8 ACTCCTCCGCGAAC 395 P8AATTGAATTTCGGTACGGGAAAGGA 396 79 F379 GTCGCGCGGATCGC 129 397 R8ACTCCTCCGCGAAC 395 P8 AATTGAATTTCGGTACGGGAAAGGA 396 80 F380TCGCGCGGATCGCG 128 398 R8 ACTCCTCCGCGAAC 395 P8AATTGAATTTCGGTACGGGAAAGGA 396 81 F381 CGCGCGGATCGCGC 127 399 R8ACTCCTCCGCGAAC 395 P8 AATTGAATTTCGGTACGGGAAAGGA 396 82 F382GCGCGGATCGCGCG 126 400 R8 ACTCCTCCGCGAAC 395 P8AATTGAATTTCGGTACGGGAAAGGA 396 83 F383 CGCGGATCGCGCGT 125 401 R8ACTCCTCCGCGAAC 395 P8 AATTGAATTTCGGTACGGGAAAGGA 396 84 F384GCGGATCGCGCGTT 124 402 R8 ACTCCTCCGCGAAC 395 P8AATTGAATTTCGGTACGGGAAAGGA 396 85 F385 CGGATCGCGCGTTT 123 403 R8ACTCCTCCGCGAAC 395 P8 AATTGAATTTCGGTACGGGAAAGGA 396 86 F386GGATCGCGCGTTTT 122 404 R8 ACTCCTCCGCGAAC 395 P8AATTGAATTTCGGTACGGGAAAGGA 396 87 F387 GATCGCGCGTTTTC 121 405 R8ACTCCTCCGCGAAC 395 P8 AATTGAATTTCGGTACGGGAAAGGA 396 88 F388ATCGCGCGTTTTCG 120 406 R8 ACTCCTCCGCGAAC 395 P8AATTGAATTTCGGTACGGGAAAGGA 396 89 F389 TCGCGCGTTTTCGT 119 407 R8ACTCCTCCGCGAAC 395 P8 AATTGAATTTCGGTACGGGAAAGGA 396 90 F390CGCGCGTTTTCGTC 118 408 R8 ACTCCTCCGCGAAC 395 P8AATTGAATTTCGGTACGGGAAAGGA 396 91 F391 GCGCGTTTTCGTCG 117 409 R8ACTCCTCCGCGAAC 395 P8 AATTGAATTTCGGTACGGGAAAGGA 396 92 F392CGCGTTTTCGTCGT 116 410 R8 ACTCCTCCGCGAAC 395 P8AATTGAATTTCGGTACGGGAAAGGA 396 93 F393 GCGTTTTCGTCGTT 115 411 R8ACTCCTCCGCGAAC 395 P8 AATTGAATTTCGGTACGGGAAAGGA 396 94 F394CGTTTTCGTCGTTT 114 412 R8 ACTCCTCCGCGAAC 395 P8AATTGAATTTCGGTACGGGAAAGGA 396 95 F395 GTTTTCGTCGTTTT 113 413 R8ACTCCTCCGCGAAC 395 P8 AATTGAATTTCGGTACGGGAAAGGA 396 96 F396TTTTCGTCGTTTTG 112 414 R8 ACTCCTCCGCGAAC 395 P8AATTGAATTTCGGTACGGGAAAGGA 396 97 F397 TTTCGTCGTTTTGT 111 415 R8ACTCCTCCGCGAAC 395 P8 AATTGAATTTCGGTACGGGAAAGGA 396 98 F398TTCGTCGTTTTGTT 110 416 R8 ACTCCTCCGCGAAC 395 P8AATTGAATTTCGGTACGGGAAAGGA 396 99 F399 TCGTCGTTTTGTTT 109 417 R8ACTCCTCCGCGAAC 395 P8 AATTGAATTTCGGTACGGGAAAGGA 396 00 F400CGTCGTTTTGTTTT 108 418 R8 ACTCCTCCGCGAAC 395 P8AATTGAATTTCGGTACGGGAAAGGA 396 01 F401 GTCGTTTTGTTTTT 107 419 R8ACTCCTCCGCGAAC 395 P8 AATTGAATTTCGGTACGGGAAAGGA 396 02 F402TCGTTTTGTTTTTA 106 420 R8 ACTCCTCCGCGAAC 395 P8AATTGAATTTCGGTACGGGAAAGGA 396 03 F403 CGTTTTGTTTTTAA 105 421 R8ACTCCTCCGCGAAC 395 P8 AATTGAATTTCGGTACGGGAAAGGA 396 04 F404GTTTTGTTTTTAAA 104 422 R8 ACTCCTCCGCGAAC 395 P8AATTGAATTTCGGTACGGGAAAGGA 396 05 F405 TTTTGTTTTTAAAT 103 423 R8ACTCCTCCGCGAAC 395 P8 AATTGAATTTCGGTACGGGAAAGGA 396 06 F406TTTGTTTTTAAATT 102 424 R8 ACTCCTCCGCGAAC 395 P8AATTGAATTTCGGTACGGGAAAGGA 396 07 F407 TTGTTTTTAAATTT 101 425 R8ACTCCTCCGCGAAC 395 P8 AATTGAATTTCGGTACGGGAAAGGA 396 08 F408TGTTTTTAAATTTT 100 426 R8 ACTCCTCCGCGAAC 395 P8AATTGAATTTCGGTACGGGAAAGGA 396 09 F409 GTTTTTAAATTTTT 99 427 R8ACTCCTCCGCGAAC 395 P8 AATTGAATTTCGGTACGGGAAAGGA 396 10 F410TTTTTAAATTTTTG 98 428 R8 ACTCCTCCGCGAAC 395 P8 AATTGAATTTCGGTACGGGAAAGGA396 11 F411 TTTTAAATTTTTGT 97 429 R8 ACTCCTCCGCGAAC 395 P8AATTGAATTTCGGTACGGGAAAGGA 396 12 F412 TTTAAATTTTTGTC 96 430 R8ACTCCTCCGCGAAC 395 P8 AATTGAATTTCGGTACGGGAAAGGA 396 13 F413TTAAATTTTTGTCG 95 431 R8 ACTCCTCCGCGAAC 395 P8 AATTGAATTTCGGTACGGGAAAGGA396 14 F414 TAAATTTTTGTCGT 94 432 R8 ACTCCTCCGCGAAC 395 P8AATTGAATTTCGGTACGGGAAAGGA 396 15 F415 AAATTTTTGTCGTA 93 433 R8ACTCCTCCGCGAAC 395 P8 AATTGAATTTCGGTACGGGAAAGGA 396 16 F416AATTTTTGTCGTAG 92 434 R8 ACTCCTCCGCGAAC 395 P8 AATTGAATTTCGGTACGGGAAAGGA396 17 F417 ATTTTTGTCGTAGT 91 435 R8 ACTCCTCCGCGAAC 395 P8AATTGAATTTCGGTACGGGAAAGGA 396 18 F418 TTTTTGTCGTAGTT 90 436 R8ACTCCTCCGCGAAC 395 P8 AATTGAATTTCGGTACGGGAAAGGA 396 19 F419TTTTGTCGTAGTTT 89 437 R8 ACTCCTCCGCGAAC 395 P8 AATTGAATTTCGGTACGGGAAAGGA396 20 F420 TTTGTCGTAGTTTT 88 438 R8 ACTCCTCCGCGAAC 395 P8AATTGAATTTCGGTACGGGAAAGGA 396 21 F421 TTGTCGTAGTTTTT 87 439 R8ACTCCTCCGCGAAC 395 P8 AATTGAATTTCGGTACGGGAAAGGA 396 22 F422TGTCGTAGTTTTTT 86 440 R8 ACTCCTCCGCGAAC 395 P8 AATTGAATTTCGGTACGGGAAAGGA396 23 F423 GTCGTAGTTTTTTT 85 441 R8 ACTCCTCCGCGAAC 395 P8AATTGAATTTCGGTACGGGAAAGGA 396 24 F424 TCGTAGTTTTTTTT 84 442 R8ACTCCTCCGCGAAC 395 P8 AATTGAATTTCGGTACGGGAAAGGA 396 25 F425CGTAGTTTTTTTTT 83 443 R8 ACTCCTCCGCGAAC 395 P8 AATTGAATTTCGGTACGGGAAAGGA396 26 F426 GTAGTTTTTTTTTA 82 444 R8 ACTCCTCCGCGAAC 395 P8AATTGAATTTCGGTACGGGAAAGGA 396 27 F427 TAGTTTTTTTTTAA 81 445 R8ACTCCTCCGCGAAC 395 P8 AATTGAATTTCGGTACGGGAAAGGA 396 28 F428AGTTTTTTTTTAAG 80 446 R8 ACTCCTCCGCGAAC 395 P8 AATTGAATTTCGGTACGGGAAAGGA396 29 F429 GTTTTTTTTTAAGT 140 447 R9 CACGAAATTAATAC 448 P9GTTTTAGAGAGTAGTTTTTTCGGA 449 30 F430 TTTTTTTTTAAGTT 139 450 R9CACGAAATTAATAC 448 P9 GTTTTAGAGAGTAGTTTTTTCGGA 449 31 F431TTTTTTTTAAGTTA 138 451 R9 CACGAAATTAATAC 448 P9 GTTTTAGAGAGTAGTTTTTTCGGA449 32 F432 TTTTTTTAAGTTAG 137 452 R9 CACGAAATTAATAC 448 P9GTTTTAGAGAGTAGTTTTTTCGGA 449 33 F433 TTTTTTAAGTTAGC 136 453 R9CACGAAATTAATAC 448 P9 GTTTTAGAGAGTAGTTTTTTCGGA 449 34 F434TTTTTAAGTTAGCG 135 454 R9 CACGAAATTAATAC 448 P9 GTTTTAGAGAGTAGTTTTTTCGGA449 35 F435 TTTTAAGTTAGCGA 134 455 R9 CACGAAATTAATAC 448 P9GTTTTAGAGAGTAGTTTTTTCGGA 449 36 F436 TTTAAGTTAGCGAA 133 456 R9CACGAAATTAATAC 448 P9 GTTTTAGAGAGTAGTTTTTTCGGA 449 37 F437TTAAGTTAGCGAAT 132 457 R9 CACGAAATTAATAC 448 P9 GTTTTAGAGAGTAGTTTTTTCGGA449 38 F438 TAAGTTAGCGAATT 131 458 R9 CACGAAATTAATAC 448 P9GTTTTAGAGAGTAGTTTTTTCGGA 449 39 F439 AAGTTAGCGAATTT 130 459 R9CACGAAATTAATAC 448 P9 GTTTTAGAGAGTAGTTTTTTCGGA 449 40 F440AGTTAGCGAATTTA 129 460 R9 CACGAAATTAATAC 448 P9 GTTTTAGAGAGTAGTTTTTTCGGA449 41 F441 GTTAGCGAATTTAT 128 461 R9 CACGAAATTAATAC 448 P9GTTTTAGAGAGTAGTTTTTTCGGA 449 42 F442 TTAGCGAATTTATT 127 462 R9CACGAAATTAATAC 448 P9 GTTTTAGAGAGTAGTTTTTTCGGA 449 43 F443TAGCGAATTTATTT 126 463 R9 CACGAAATTAATAC 448 P9 GTTTTAGAGAGTAGTTTTTTCGGA449 44 F444 AGCGAATTTATTTT 125 464 R9 CACGAAATTAATAC 448 P9GTTTTAGAGAGTAGTTTTTTCGGA 449 45 F445 GCGAATTTATTTTT 124 465 R9CACGAAATTAATAC 448 P9 GTTTTAGAGAGTAGTTTTTTCGGA 449 46 F446CGAATTTATTTTTT 123 466 R9 CACGAAATTAATAC 448 P9 GTTTTAGAGAGTAGTTTTTTCGGA449 47 F447 GAATTTATTTTTTA 122 467 R9 CACGAAATTAATAC 448 P9GTTTTAGAGAGTAGTTTTTTCGGA 449 48 F448 AATTTATTTTTTAA 121 468 R9CACGAAATTAATAC 448 P9 GTTTTAGAGAGTAGTTTTTTCGGA 449 49 F449ATTTATTTTTTAAA 120 469 R9 CACGAAATTAATAC 448 P9 GTTTTAGAGAGTAGTTTTTTCGGA449 50 F450 TTTATTTTTTAAAA 119 470 R9 CACGAAATTAATAC 448 P9GTTTTAGAGAGTAGTTTTTTCGGA 449 51 F451 TTATTTTTTAAAAT 118 471 R9CACGAAATTAATAC 448 P9 GTTTTAGAGAGTAGTTTTTTCGGA 449 52 F452TATTTTTTAAAATT 117 472 R9 CACGAAATTAATAC 448 P9 GTTTTAGAGAGTAGTTTTTTCGGA449 53 F453 ATTTTTTAAAATTA 116 473 R9 CACGAAATTAATAC 448 P9GTTTTAGAGAGTAGTTTTTTCGGA 449 54 F454 TTTTTTAAAATTAG 115 474 R9CACGAAATTAATAC 448 P9 GTTTTAGAGAGTAGTTTTTTCGGA 449 55 F455TTTTTAAAATTAGA 114 475 R9 CACGAAATTAATAC 448 P9 GTTTTAGAGAGTAGTTTTTTCGGA449 56 F456 TTTTAAAATTAGAA 113 476 R9 CACGAAATTAATAC 448 P9GTTTTAGAGAGTAGTTTTTTCGGA 449 57 F457 TTTAAAATTAGAAA 112 477 R9CACGAAATTAATAC 448 P9 GTTTTAGAGAGTAGTTTTTTCGGA 449 58 F458TTAAAATTAGAAAT 111 478 R9 CACGAAATTAATAC 448 P9 GTTTTAGAGAGTAGTTTTTTCGGA449 59 F459 TAAAATTAGAAATT 110 479 R9 CACGAAATTAATAC 448 P9GTTTTAGAGAGTAGTTTTTTCGGA 449 60 F460 AAAATTAGAAATTG 109 480 R9CACGAAATTAATAC 448 P9 GTTTTAGAGAGTAGTTTTTTCGGA 449 61 F461AAATTAGAAATTGA 108 481 R9 CACGAAATTAATAC 448 P9 GTTTTAGAGAGTAGTTTTTTCGGA449 62 F462 AATTAGAAATTGAA 107 482 R9 CACGAAATTAATAC 448 P9GTTTTAGAGAGTAGTTTTTTCGGA 449 63 F463 ATTAGAAATTGAAT 106 483 R9CACGAAATTAATAC 448 P9 GTTTTAGAGAGTAGTTTTTTCGGA 449 64 F464TTAGAAATTGAATT 105 484 R9 CACGAAATTAATAC 448 P9 GTTTTAGAGAGTAGTTTTTTCGGA449 65 F465 TAGAAATTGAATTT 104 485 R9 CACGAAATTAATAC 448 P9GTTTTAGAGAGTAGTTTTTTCGGA 449 66 F466 AGAAATTGAATTTC 103 486 R9CACGAAATTAATAC 448 P9 GTTTTAGAGAGTAGTTTTTTCGGA 449 67 F467GAAATTGAATTTCG 102 487 R9 CACGAAATTAATAC 448 P9 GTTTTAGAGAGTAGTTTTTTCGGA449 68 F468 AAATTGAATTTCGG 101 488 R9 CACGAAATTAATAC 448 P9GTTTTAGAGAGTAGTTTTTTCGGA 449 69 F469 AATTGAATTTCGGT 100 489 R9CACGAAATTAATAC 448 P9 GTTTTAGAGAGTAGTTTTTTCGGA 449 70 F470ATTGAATTTCGGTA 99 490 R9 CACGAAATTAATAC 448 P9 GTTTTAGAGAGTAGTTTTTTCGGA449 71 F471 TTGAATTTCGGTAC 98 491 R9 CACGAAATTAATAC 448 P9GTTTTAGAGAGTAGTTTTTTCGGA 449 72 F472 TGAATTTCGGTACG 97 492 R9CACGAAATTAATAC 448 P9 GTTTTAGAGAGTAGTTTTTTCGGA 449 73 F473GAATTTCGGTACGG 96 493 R9 CACGAAATTAATAC 448 P9 GTTTTAGAGAGTAGTTTTTTCGGA449 74 F474 AATTTCGGTACGGG 95 494 R9 CACGAAATTAATAC 448 P9GTTTTAGAGAGTAGTTTTTTCGGA 449 75 F475 ATTTCGGTACGGGA 94 495 R9CACGAAATTAATAC 448 P9 GTTTTAGAGAGTAGTTTTTTCGGA 449 76 F476TTTCGGTACGGGAA 93 496 R9 CACGAAATTAATAC 448 P9 GTTTTAGAGAGTAGTTTTTTCGGA449 77 F477 TTCGGTACGGGAAA 92 497 R9 CACGAAATTAATAC 448 P9GTTTTAGAGAGTAGTTTTTTCGGA 449 78 F478 TCGGTACGGGAAAG 91 498 R9CACGAAATTAATAC 448 P9 GTTTTAGAGAGTAGTTTTTTCGGA 449 79 F479CGGTACGGGAAAGG 90 499 R9 CACGAAATTAATAC 448 P9 GTTTTAGAGAGTAGTTTTTTCGGA449 80 F480 GGTACGGGAAAGGA 89 500 R9 CACGAAATTAATAC 448 P9GTTTTAGAGAGTAGTTTTTTCGGA 449 81 F481 GTACGGGAAAGGAG 88 501 R9CACGAAATTAATAC 448 P9 GTTTTAGAGAGTAGTTTTTTCGGA 449 82 F482TACGGGAAAGGAGT 87 502 R9 CACGAAATTAATAC 448 P9 GTTTTAGAGAGTAGTTTTTTCGGA449 83 F483 ACGGGAAAGGAGTT 86 503 R9 CACGAAATTAATAC 448 P9GTTTTAGAGAGTAGTTTTTTCGGA 449 84 F484 CGGGAAAGGAGTTC 85 504 R9CACGAAATTAATAC 448 P9 GTTTTAGAGAGTAGTTTTTTCGGA 449 85 F485GGGAAAGGAGTTCG 84 505 R9 CACGAAATTAATAC 448 P9 GTTTTAGAGAGTAGTTTTTTCGGA449 86 F486 GGAAAGGAGTTCGC 83 506 R9 CACGAAATTAATAC 448 P9GTTTTAGAGAGTAGTTTTTTCGGA 449 87 F487 GAAAGGAGTTCGCG 82 507 R9CACGAAATTAATAC 448 P9 GTTTTAGAGAGTAGTTTTTTCGGA 449 88 F488AAAGGAGTTCGCGG 81 508 R9 CACGAAATTAATAC 448 P9 GTTTTAGAGAGTAGTTTTTTCGGA449 89 F489 AAGGAGTTCGCGGA 80 509 R9 CACGAAATTAATAC 448 P9GTTTTAGAGAGTAGTTTTTTCGGA 449 90 F490 AGGAGTTCGCGGAG 140 510 R10CGCCCGCAACTACG 511 P10 GTGAGAGGGCGTCGCGTTTTCGGGG 512 91 F491GGAGTTCGCGGAGG 139 513 R10 CGCCCGCAACTACG 511 P10GTGAGAGGGCGTCGCGTTTTCGGGG 512 92 F492 GAGTTCGCGGAGGA 138 514 R10CGCCCGCAACTACG 511 P10 GTGAGAGGGCGTCGCGTTTTCGGGG 512 93 F493AGTTCGCGGAGGAG 137 515 R10 CGCCCGCAACTACG 511 P10GTGAGAGGGCGTCGCGTTTTCGGGG 512 94 F494 GTTCGCGGAGGAGT 136 516 R10CGCCCGCAACTACG 511 P10 GTGAGAGGGCGTCGCGTTTTCGGGG 512 95 F495TTCGCGGAGGAGTA 135 517 R10 CGCCCGCAACTACG 511 P10GTGAGAGGGCGTCGCGTTTTCGGGG 512 96 F496 TCGCGGAGGAGTAA 134 518 R10CGCCCGCAACTACG 511 P10 GTGAGAGGGCGTCGCGTTTTCGGGG 512 97 F497CGCGGAGGAGTAAA 133 519 R10 CGCCCGCAACTACG 511 P10GTGAGAGGGCGTCGCGTTTTCGGGG 512 98 F498 GCGGAGGAGTAAAA 132 520 R10CGCCCGCAACTACG 511 P10 GTGAGAGGGCGTCGCGTTTTCGGGG 512 99 F499CGGAGGAGTAAAAT 131 521 R10 CGCCCGCAACTACG 511 P10GTGAGAGGGCGTCGCGTTTTCGGGG 512 00 F500 GGAGGAGTAAAATT 130 522 R10CGCCCGCAACTACG 511 P10 GTGAGAGGGCGTCGCGTTTTCGGGG 512 01 F501GAGGAGTAAAATTA 129 523 R10 CGCCCGCAACTACG 511 P10GTGAGAGGGCGTCGCGTTTTCGGGG 512 02 F502 AGGAGTAAAATTAT 128 524 R10CGCCCGCAACTACG 511 P10 GTGAGAGGGCGTCGCGTTTTCGGGG 512 03 F503GGAGTAAAATTATA 127 525 R10 CGCCCGCAACTACG 511 P10GTGAGAGGGCGTCGCGTTTTCGGGG 512 04 F504 GAGTAAAATTATAG 126 526 R10CGCCCGCAACTACG 511 P10 GTGAGAGGGCGTCGCGTTTTCGGGG 512 05 F505AGTAAAATTATAGT 125 527 R10 CGCCCGCAACTACG 511 P10GTGAGAGGGCGTCGCGTTTTCGGGG 512 06 F506 GTAAAATTATAGTA 124 528 R10CGCCCGCAACTACG 511 P10 GTGAGAGGGCGTCGCGTTTTCGGGG 512 07 F507TAAAATTATAGTAG 123 529 R10 CGCCCGCAACTACG 511 P10GTGAGAGGGCGTCGCGTTTTCGGGG 512 08 F508 AAAATTATAGTAGA 122 530 R10CGCCCGCAACTACG 511 P10 GTGAGAGGGCGTCGCGTTTTCGGGG 512 09 F509AAATTATAGTAGAG 121 531 R10 CGCCCGCAACTACG 511 P10GTGAGAGGGCGTCGCGTTTTCGGGG 512 10 F510 AATTATAGTAGAGT 120 532 R10CGCCCGCAACTACG 511 P10 GTGAGAGGGCGTCGCGTTTTCGGGG 512 11 F511ATTATAGTAGAGTA 119 533 R10 CGCCCGCAACTACG 511 P10GTGAGAGGGCGTCGCGTTTTCGGGG 512 12 F512 TTATAGTAGAGTAA 118 534 R10CGCCCGCAACTACG 511 P10 GTGAGAGGGCGTCGCGTTTTCGGGG 512 13 F513TATAGTAGAGTAAG 117 535 R10 CGCCCGCAACTACG 511 P10GTGAGAGGGCGTCGCGTTTTCGGGG 512 14 F514 ATAGTAGAGTAAGA 116 536 R10CGCCCGCAACTACG 511 P10 GTGAGAGGGCGTCGCGTTTTCGGGG 512 15 F515TAGTAGAGTAAGAA 115 537 R10 CGCCCGCAACTACG 511 P10GTGAGAGGGCGTCGCGTTTTCGGGG 512 16 F516 AGTAGAGTAAGAAG 114 538 R10CGCCCGCAACTACG 511 P10 GTGAGAGGGCGTCGCGTTTTCGGGG 512 17 F517GTAGAGTAAGAAGA 113 539 R10 CGCCCGCAACTACG 511 P10GTGAGAGGGCGTCGCGTTTTCGGGG 512 18 F518 TAGAGTAAGAAGAG 112 540 R10CGCCCGCAACTACG 511 P10 GTGAGAGGGCGTCGCGTTTTCGGGG 512 19 F519AGAGTAAGAAGAGT 111 541 R10 CGCCCGCAACTACG 511 P10GTGAGAGGGCGTCGCGTTTTCGGGG 512 20 F520 GAGTAAGAAGAGTT 110 542 R10CGCCCGCAACTACG 511 P10 GTGAGAGGGCGTCGCGTTTTCGGGG 512 21 F521AGTAAGAAGAGTTT 109 543 R10 CGCCCGCAACTACG 511 P10GTGAGAGGGCGTCGCGTTTTCGGGG 512 22 F522 GTAAGAAGAGTTTT 108 544 R10CGCCCGCAACTACG 511 P10 GTGAGAGGGCGTCGCGTTTTCGGGG 512 23 F523TAAGAAGAGTTTTA 107 545 R10 CGCCCGCAACTACG 511 P10GTGAGAGGGCGTCGCGTTTTCGGGG 512 24 F524 AAGAAGAGTTTTAG 106 546 R10CGCCCGCAACTACG 511 P10 GTGAGAGGGCGTCGCGTTTTCGGGG 512 25 F525AGAAGAGTTTTAGA 105 547 R10 CGCCCGCAACTACG 511 P10GTGAGAGGGCGTCGCGTTTTCGGGG 512 26 F526 GAAGAGTTTTAGAG 104 548 R10CGCCCGCAACTACG 511 P10 GTGAGAGGGCGTCGCGTTTTCGGGG 512 27 F527AAGAGTTTTAGAGA 103 549 R10 CGCCCGCAACTACG 511 P10GTGAGAGGGCGTCGCGTTTTCGGGG 512 28 F528 AGAGTTTTAGAGAG 102 550 R10CGCCCGCAACTACG 511 P10 GTGAGAGGGCGTCGCGTTTTCGGGG 512 29 F529GAGTTTTAGAGAGT 101 551 R10 CGCCCGCAACTACG 511 P10GTGAGAGGGCGTCGCGTTTTCGGGG 512 30 F530 AGTTTTAGAGAGTA 100 552 R10CGCCCGCAACTACG 511 P10 GTGAGAGGGCGTCGCGTTTTCGGGG 512 31 F531GTTTTAGAGAGTAG 99 553 R10 CGCCCGCAACTACG 511 P10GTGAGAGGGCGTCGCGTTTTCGGGG 512 32 F532 TTTTAGAGAGTAGT 98 554 R10CGCCCGCAACTACG 511 P10 GTGAGAGGGCGTCGCGTTTTCGGGG 512 33 F533TTTAGAGAGTAGTT 97 555 R10 CGCCCGCAACTACG 511 P10GTGAGAGGGCGTCGCGTTTTCGGGG 512 34 F534 TTAGAGAGTAGTTT 96 556 R10CGCCCGCAACTACG 511 P10 GTGAGAGGGCGTCGCGTTTTCGGGG 512 35 F535TAGAGAGTAGTTTT 95 557 R10 CGCCCGCAACTACG 511 P10GTGAGAGGGCGTCGCGTTTTCGGGG 512 36 F536 AGAGAGTAGTTTTT 94 558 R10CGCCCGCAACTACG 511 P10 GTGAGAGGGCGTCGCGTTTTCGGGG 512 37 F537GAGAGTAGTTTTTT 93 559 R10 CGCCCGCAACTACG 511 P10GTGAGAGGGCGTCGCGTTTTCGGGG 512 38 F538 AGAGTAGTTTTTTC 92 560 R10CGCCCGCAACTACG 511 P10 GTGAGAGGGCGTCGCGTTTTCGGGG 512 39 F539GAGTAGTTTTTTCG 91 561 R10 CGCCCGCAACTACG 511 P10GTGAGAGGGCGTCGCGTTTTCGGGG 512 40 F540 AGTAGTTTTTTCGG 90 562 R10CGCCCGCAACTACG 511 P10 GTGAGAGGGCGTCGCGTTTTCGGGG 512 41 F541GTAGTTTTTTCGGA 89 563 R10 CGCCCGCAACTACG 511 P10GTGAGAGGGCGTCGCGTTTTCGGGG 512 42 F542 TAGTTTTTTCGGAG 88 564 R10CGCCCGCAACTACG 511 P10 GTGAGAGGGCGTCGCGTTTTCGGGG 512 43 F543AGTTTTTTCGGAGT 87 565 R10 CGCCCGCAACTACG 511 P10GTGAGAGGGCGTCGCGTTTTCGGGG 512 44 F544 GTTTTTTCGGAGTA 86 566 R10CGCCCGCAACTACG 511 P10 GTGAGAGGGCGTCGCGTTTTCGGGG 512 45 F545TTTTTTCGGAGTAT 85 567 R10 CGCCCGCAACTACG 511 P10GTGAGAGGGCGTCGCGTTTTCGGGG 512 46 F546 TTTTTCGGAGTATT 84 568 R10CGCCCGCAACTACG P10 GTGAGAGGGCGTCGCGTTTTCGGGG 512 47 F547 TTTTCGGAGTATTA83 569 R10 CGCCCGCAACTACG 511 P10 GTGAGAGGGCGTCGCGTTTTCGGGG 512 48 F548TTTCGGAGTATTAA 82 570 R10 CGCCCGCAACTACG 511 P10GTGAGAGGGCGTCGCGTTTTCGGGG 512 49 F549 TTCGGAGTATTAAT 81 571 R10CGCCCGCAACTACG 511 P10 GTGAGAGGGCGTCGCGTTTTCGGGG 512 50 F550TCGGAGTATTAATT 80 572 R10 CGCCCGCAACTACG 511 P10GTGAGAGGGCGTCGCGTTTTCGGGG 512 51 F551 CGGAGTATTAATTT 140 573 R11CTCGAAAACTCGAA 574 P11 AAGCGAGCGTTTTCGAGTTTCGAG 575 52 F552GGAGTATTAATTTC 139 576 R11 CTCGAAAACTCGAA 574 P11AAGCGAGCGTTTTCGAGTTTCGAG 575 53 F553 GAGTATTAATTTCG 138 577 R11CTCGAAAACTCGAA 574 P11 AAGCGAGCGTTTTCGAGTTTCGAG 575 54 F554AGTATTAATTTCGT 137 578 R11 CTCGAAAACTCGAA 574 P11AAGCGAGCGTTTTCGAGTTTCGAG 575 55 F555 GTATTAATTTCGTG 136 579 R11CTCGAAAACTCGAA 574 P11 AAGCGAGCGTTTTCGAGTTTCGAG 575 56 F556TATTAATTTCGTGT 135 580 R11 CTCGAAAACTCGAA 574 P11AAGCGAGCGTTTTCGAGTTTCGAG 575 57 F557 ATTAATTTCGTGTC 134 581 R11CTCGAAAACTCGAA 574 P11 AAGCGAGCGTTTTCGAGTTTCGAG 575 58 F558TTAATTTCGTGTCG 133 582 R11 CTCGAAAACTCGAA 574 P11AAGCGAGCGTTTTCGAGTTTCGAG 575 59 F559 TAATTTCGTGTCGG 132 583 R11CTCGAAAACTCGAA 574 P11 AAGCGAGCGTTTTCGAGTTTCGAG 575 60 F560AATTTCGTGTCGGG 131 584 R11 CTCGAAAACTCGAA 574 P11AAGCGAGCGTTTTCGAGTTTCGAG 575 61 F561 ATTTCGTGTCGGGA 130 585 R11CTCGAAAACTCGAA 574 P11 AAGCGAGCGTTTTCGAGTTTCGAG 575 62 F562TTTCGTGTCGGGAG 129 586 R11 CTCGAAAACTCGAA 574 P11AAGCGAGCGTTTTCGAGTTTCGAG 575 63 F563 TTCGTGTCGGGAGT 128 587 R11CTCGAAAACTCGAA 574 P11 AAGCGAGCGTTTTCGAGTTTCGAG 575 64 F564TCGTGTCGGGAGTG 127 588 R11 CTCGAAAACTCGAA 574 P11AAGCGAGCGTTTTCGAGTTTCGAG 575 65 F565 CGTGTCGGGAGTGT 126 589 R11CTCGAAAACTCGAA 574 P11 AAGCGAGCGTTTTCGAGTTTCGAG 575 66 F566GTGTCGGGAGTGTA 125 590 R11 CTCGAAAACTCGAA 574 P11AAGCGAGCGTTTTCGAGTTTCGAG 575 67 F567 TGTCGGGAGTGTAG 124 591 R11CTCGAAAACTCGAA 574 P11 AAGCGAGCGTTTTCGAGTTTCGAG 575 68 F568GTCGGGAGTGTAGA 123 592 R11 CTCGAAAACTCGAA 574 P11AAGCGAGCGTTTTCGAGTTTCGAG 575 69 F569 TCGGGAGTGTAGAA 122 593 R11CTCGAAAACTCGAA 574 P11 AAGCGAGCGTTTTCGAGTTTCGAG 575 70 F570CGGGAGTGTAGAAA 121 594 R11 CTCGAAAACTCGAA 574 P11AAGCGAGCGTTTTCGAGTTTCGAG 575 71 F571 GGGAGTGTAGAAAT 120 595 R11CTCGAAAACTCGAA 574 P11 AAGCGAGCGTTTTCGAGTTTCGAG 575 72 F572GGAGTGTAGAAATT 119 596 R11 CTCGAAAACTCGAA 574 P11AAGCGAGCGTTTTCGAGTTTCGAG 575 73 F573 GAGTGTAGAAATTA 118 597 R11CTCGAAAACTCGAA 574 P11 AAGCGAGCGTTTTCGAGTTTCGAG 575 74 F574AGTGTAGAAATTAA 117 598 R11 CTCGAAAACTCGAA 574 P11AAGCGAGCGTTTTCGAGTTTCGAG 575 75 F575 GTGTAGAAATTAAT 116 599 R11CTCGAAAACTCGAA 574 P11 AAGCGAGCGTTTTCGAGTTTCGAG 575 76 F576TGTAGAAATTAATA 115 600 R11 CTCGAAAACTCGAA 574 P11AAGCGAGCGTTTTCGAGTTTCGAG 575 77 F577 GTAGAAATTAATAA 114 601 R11CTCGAAAACTCGAA 574 P11 AAGCGAGCGTTTTCGAGTTTCGAG 575 78 F578TAGAAATTAATAAG 113 602 R11 CTCGAAAACTCGAA 574 P11AAGCGAGCGTTTTCGAGTTTCGAG 575 79 F579 AGAAATTAATAAGT 112 603 R11CTCGAAAACTCGAA 574 P11 AAGCGAGCGTTTTCGAGTTTCGAG 575 80 F580GAAATTAATAAGTG 111 604 R11 CTCGAAAACTCGAA 574 P11AAGCGAGCGTTTTCGAGTTTCGAG 575 81 F581 AAATTAATAAGTGA 110 605 R11CTCGAAAACTCGAA 574 P11 AAGCGAGCGTTTTCGAGTTTCGAG 575 82 F582AATTAATAAGTGAG 109 606 R11 CTCGAAAACTCGAA 574 P11AAGCGAGCGTTTTCGAGTTTCGAG 575 83 F583 ATTAATAAGTGAGA 108 607 R11CTCGAAAACTCGAA 574 P11 AAGCGAGCGTTTTCGAGTTTCGAG 575 84 F584TTAATAAGTGAGAG 107 608 R11 CTCGAAAACTCGAA 574 P11AAGCGAGCGTTTTCGAGTTTCGAG 575 85 F585 TAATAAGTGAGAGG 106 609 R11CTCGAAAACTCGAA 574 P11 AAGCGAGCGTTTTCGAGTTTCGAG 575 86 F586AATAAGTGAGAGGG 105 610 R11 CTCGAAAACTCGAA 574 P11AAGCGAGCGTTTTCGAGTTTCGAG 575 87 F587 ATAAGTGAGAGGGC 104 611 R11CTCGAAAACTCGAA 574 P11 AAGCGAGCGTTTTCGAGTTTCGAG 575 88 F588TAAGTGAGAGGGCG 103 612 R11 CTCGAAAACTCGAA 574 P11AAGCGAGCGTTTTCGAGTTTCGAG 575 89 F589 AAGTGAGAGGGCGT 102 613 R11CTCGAAAACTCGAA 574 P11 AAGCGAGCGTTTTCGAGTTTCGAG 575 90 F590AGTGAGAGGGCGTC 101 614 R11 CTCGAAAACTCGAA 574 P11AAGCGAGCGTTTTCGAGTTTCGAG 575 91 F591 GTGAGAGGGCGTCG 100 615 R11CTCGAAAACTCGAA 574 P11 AAGCGAGCGTTTTCGAGTTTCGAG 575 92 F592TGAGAGGGCGTCGC 99 616 R11 CTCGAAAACTCGAA 574 P11AAGCGAGCGTTTTCGAGTTTCGAG 575 93 F593 GAGAGGGCGTCGCG 98 617 R11CTCGAAAACTCGAA 574 P11 AAGCGAGCGTTTTCGAGTTTCGAG 575 94 F594AGAGGGCGTCGCGT 79 618 R11 CTCGAAAACTCGAA 574 P11AAGCGAGCGTTTTCGAGTTTCGAG 575 95 F595 GAGGGCGTCGCGTT 96 619 R11CTCGAAAACTCGAA 574 P11 AAGCGAGCGTTTTCGAGTTTCGAG 575 96 F596AGGGCGTCGCGTTT 95 620 R11 CTCGAAAACTCGAA 574 P11AAGCGAGCGTTTTCGAGTTTCGAG 575 97 F597 GGGCGTCGCGTTTT 94 621 R11CTCGAAAACTCGAA 574 P11 AAGCGAGCGTTTTCGAGTTTCGAG 575 98 F598GGCGTCGCGTTTTC 93 622 R11 CTCGAAAACTCGAA 574 P11AAGCGAGCGTTTTCGAGTTTCGAG 575 99 F599 GCGTCGCGTTTTCG 92 623 R11CTCGAAAACTCGAA 574 P11 AAGCGAGCGTTTTCGAGTTTCGAG 575 00 F600CGTCGCGTTTTCGG 91 624 R11 CTCGAAAACTCGAA 574 P11AAGCGAGCGTTTTCGAGTTTCGAG 575 01 F601 GTCGCGTTTTCGGG 90 625 R11CTCGAAAACTCGAA 574 P11 AAGCGAGCGTTTTCGAGTTTCGAG 575 02 F602TCGCGTTTTCGGGG 89 626 R11 CTCGAAAACTCGAA 574 P11AAGCGAGCGTTTTCGAGTTTCGAG 575 03 F603 CGCGTTTTCGGGGC 88 627 R11CTCGAAAACTCGAA 574 P11 AAGCGAGCGTTTTCGAGTTTCGAG 575 04 F604GCGTTTTCGGGGCG 87 628 R11 CTCGAAAACTCGAA 574 P11AAGCGAGCGTTTTCGAGTTTCGAG 575 05 F605 CGTTTTCGGGGCGT 86 629 R11CTCGAAAACTCGAA 574 P11 AAGCGAGCGTTTTCGAGTTTCGAG 575 06 F606GTTTTCGGGGCGTA 85 630 R11 CTCGAAAACTCGAA 574 P11AAGCGAGCGTTTTCGAGTTTCGAG 575 07 F607 TTTTCGGGGCGTAG 84 631 R11CTCGAAAACTCGAA 574 P11 AAGCGAGCGTTTTCGAGTTTCGAG 575 08 F608TTTCGGGGCGTAGT 83 632 R11 CTCGAAAACTCGAA 574 P11AAGCGAGCGTTTTCGAGTTTCGAG 575 09 F609 TTCGGGGCGTAGTT 82 633 R11CTCGAAAACTCGAA 574 P11 AAGCGAGCGTTTTCGAGTTTCGAG 575 10 F610TCGGGGCGTAGTTG 81 634 R11 CTCGAAAACTCGAA 574 P11AAGCGAGCGTTTTCGAGTTTCGAG 575 11 F611 CGGGGCGTAGTTGC 80 635 R11CTCGAAAACTCGAA 574 P11 AAGCGAGCGTTTTCGAGTTTCGAG 575 12 F612GGGGCGTAGTTGCG 140 636 R12 CGCTCGACGCAACC 637 P12TATTTTGTTTCGGATTCGTGTGCGCG 638 13 F613 GGGCGTAGTTGCGG 139 639 R12CGCTCGACGCAACC 637 P12 TATTTTGTTTCGGATTCGTGTGCGCG 638 14 F614GGCGTAGTTGCGGG 138 640 R12 CGCTCGACGCAACC 637 P12TATTTTGTTTCGGATTCGTGTGCGCG 638 15 F615 GCGTAGTTGCGGGC 137 641 R12CGCTCGACGCAACC 637 P12 TATTTTGTTTCGGATTCGTGTGCGCG 638 16 F616CGTAGTTGCGGGCG 136 642 R12 CGCTCGACGCAACC 637 P12TATTTTGTTTCGGATTCGTGTGCGCG 638 17 F617 GTAGTTGCGGGCGG 135 643 R12CGCTCGACGCAACC 637 P12 TATTTTGTTTCGGATTCGTGTGCGCG 638 18 F618TAGTTGCGGGCGGC 134 644 R12 CGCTCGACGCAACC 637 P12TATTTTGTTTCGGATTCGTGTGCGCG 638 19 F619 AGTTGCGGGCGGCG 133 645 R12CGCTCGACGCAACC 637 P12 TATTTTGTTTCGGATTCGTGTGCGCG 638 20 F620GTTGCGGGCGGCGG 132 646 R12 CGCTCGACGCAACC 637 P12TATTTTGTTTCGGATTCGTGTGCGCG 638 21 F621 TTGCGGGCGGCGGG 131 647 R12CGCTCGACGCAACC 637 P12 TATTTTGTTTCGGATTCGTGTGCGCG 638 22 F622TGCGGGCGGCGGGA 130 648 R12 CGCTCGACGCAACC 637 P12TATTTTGTTTCGGATTCGTGTGCGCG 638 23 F623 GCGGGCGGCGGGAG 129 649 R12CGCTCGACGCAACC 637 P12 TATTTTGTTTCGGATTCGTGTGCGCG 638 24 F624CGGGCGGCGGGAGT 128 650 R12 CGCTCGACGCAACC 637 P12TATTTTGTTTCGGATTCGTGTGCGCG 638 25 F625 GGGCGGCGGGAGTA 127 651 R12CGCTCGACGCAACC 637 P12 TATTTTGTTTCGGATTCGTGTGCGCG 638 26 F626GGCGGCGGGAGTAG 126 652 R12 CGCTCGACGCAACC 637 P12TATTTTGTTTCGGATTCGTGTGCGCG 638 27 F627 GCGGCGGGAGTAGG 125 653 R12CGCTCGACGCAACC 637 P12 TATTTTGTTTCGGATTCGTGTGCGCG 638 28 F628CGGCGGGAGTAGGC 124 654 R12 CGCTCGACGCAACC 637 P12TATTTTGTTTCGGATTCGTGTGCGCG 638 29 F629 GGCGGGAGTAGGCG 123 655 R12CGCTCGACGCAACC 637 P12 TATTTTGTTTCGGATTCGTGTGCGCG 638 30 F630GCGGGAGTAGGCGT 122 656 R12 CGCTCGACGCAACC 637 P12TATTTTGTTTCGGATTCGTGTGCGCG 638 31 F631 CGGGAGTAGGCGTA 121 657 R12CGCTCGACGCAACC 637 P12 TATTTTGTTTCGGATTCGTGTGCGCG 638 32 F632GGGAGTAGGCGTAG 120 658 R12 CGCTCGACGCAACC 637 P12TATTTTGTTTCGGATTCGTGTGCGCG 638 33 F633 GGAGTAGGCGTAGG 119 659 R12CGCTCGACGCAACC 637 P12 TATTTTGTTTCGGATTCGTGTGCGCG 638 34 F634GAGTAGGCGTAGGA 118 660 R12 CGCTCGACGCAACC 637 P12TATTTTGTTTCGGATTCGTGTGCGCG 638 35 F635 AGTAGGCGTAGGAG 117 661 R12CGCTCGACGCAACC 637 P12 TATTTTGTTTCGGATTCGTGTGCGCG 638 36 F636GTAGGCGTAGGAGG 116 662 R12 CGCTCGACGCAACC 637 P12TATTTTGTTTCGGATTCGTGTGCGCG 638 37 F637 TAGGCGTAGGAGGA 115 663 R12CGCTCGACGCAACC 637 P12 TATTTTGTTTCGGATTCGTGTGCGCG 638 38 F638AGGCGTAGGAGGAG 114 664 R12 CGCTCGACGCAACC 637 P12TATTTTGTTTCGGATTCGTGTGCGCG 638 39 F639 GGCGTAGGAGGAGG 113 665 R12CGCTCGACGCAACC 637 P12 TATTTTGTTTCGGATTCGTGTGCGCG 638 40 F640GCGTAGGAGGAGGA 112 666 R12 CGCTCGACGCAACC 637 P12TATTTTGTTTCGGATTCGTGTGCGCG 638 41 F641 CGTAGGAGGAGGAA 111 667 R12CGCTCGACGCAACC 637 P12 TATTTTGTTTCGGATTCGTGTGCGCG 638 42 F642GTAGGAGGAGGAAG 110 668 R12 CGCTCGACGCAACC 637 P12TATTTTGTTTCGGATTCGTGTGCGCG 638 43 F643 TAGGAGGAGGAAGC 109 669 R12CGCTCGACGCAACC 637 P12 TATTTTGTTTCGGATTCGTGTGCGCG 638 44 F644AGGAGGAGGAAGCG 108 670 R12 CGCTCGACGCAACC 637 P12TATTTTGTTTCGGATTCGTGTGCGCG 638 45 F645 GGAGGAGGAAGCGA 107 671 R12CGCTCGACGCAACC 637 P12 TATTTTGTTTCGGATTCGTGTGCGCG 638 46 F646GAGGAGGAAGCGAG 106 672 R12 CGCTCGACGCAACC 637 P12TATTTTGTTTCGGATTCGTGTGCGCG 638 47 F647 AGGAGGAAGCGAGC 105 673 R12CGCTCGACGCAACC 637 P12 TATTTTGTTTCGGATTCGTGTGCGCG 638 48 F648GGAGGAAGCGAGCG 104 674 R12 CGCTCGACGCAACC 637 P12TATTTTGTTTCGGATTCGTGTGCGCG 638 49 F649 GAGGAAGCGAGCGT 103 675 R12CGCTCGACGCAACC 637 P12 TATTTTGTTTCGGATTCGTGTGCGCG 638 50 F650AGGAAGCGAGCGTT 102 676 R12 CGCTCGACGCAACC 637 P12TATTTTGTTTCGGATTCGTGTGCGCG 638 51 F651 GGAAGCGAGCGTTT 101 677 R12CGCTCGACGCAACC 637 P12 TATTTTGTTTCGGATTCGTGTGCGCG 638 52 F652GAAGCGAGCGTTTT 100 678 R12 CGCTCGACGCAACC 637 P12TATTTTGTTTCGGATTCGTGTGCGCG 638 53 F653 AAGCGAGCGTTTTC 99 679 R12CGCTCGACGCAACC 637 P12 TATTTTGTTTCGGATTCGTGTGCGCG 638 54 F654AGCGAGCGTTTTCG 98 680 R12 CGCTCGACGCAACC 637 P12TATTTTGTTTCGGATTCGTGTGCGCG 638 55 F655 GCGAGCGTTTTCGA 97 681 R12CGCTCGACGCAACC 637 P12 TATTTTGTTTCGGATTCGTGTGCGCG 638 56 F656CGAGCGTTTTCGAG 96 682 R12 CGCTCGACGCAACC 637 P12TATTTTGTTTCGGATTCGTGTGCGCG 638 57 F657 GAGCGTTTTCGAGT 95 683 R12CGCTCGACGCAACC 637 P12 TATTTTGTTTCGGATTCGTGTGCGCG 638 58 F658AGCGTTTTCGAGTT 94 684 R12 CGCTCGACGCAACC 637 P12TATTTTGTTTCGGATTCGTGTGCGCG 638 59 F659 GCGTTTTCGAGTTT 93 685 R12CGCTCGACGCAACC 637 P12 TATTTTGTTTCGGATTCGTGTGCGCG 638 60 F660CGTTTTCGAGTTTC 92 686 R12 CGCTCGACGCAACC 637 P12TATTTTGTTTCGGATTCGTGTGCGCG 638 61 F661 GTTTTCGAGTTTCG 91 687 R12CGCTCGACGCAACC 637 P12 TATTTTGTTTCGGATTCGTGTGCGCG 638 62 F662TTTTCGAGTTTCGA 90 688 R12 CGCTCGACGCAACC 637 P12TATTTTGTTTCGGATTCGTGTGCGCG 638 63 F663 TTTCGAGTTTCGAG 89 689 R12CGCTCGACGCAACC 637 P12 TATTTTGTTTCGGATTCGTGTGCGCG 638 64 F664TTCGAGTTTCGAGT 88 690 R12 CGCTCGACGCAACC 637 P12TATTTTGTTTCGGATTCGTGTGCGCG 638 65 F665 TCGAGTTTCGAGTT 87 691 R12CGCTCGACGCAACC 637 P12 TATTTTGTTTCGGATTCGTGTGCGCG 638 66 F666CGAGTTTCGAGTTC 86 692 R12 CGCTCGACGCAACC 637 P12TATTTTGTTTCGGATTCGTGTGCGCG 638 67 F667 GAGTTTCGAGTTCG 85 693 R12CGCTCGACGCAACC 637 P12 TATTTTGTTTCGGATTCGTGTGCGCG 638 68 F668AGTTTCGAGTTCGA 84 694 R12 CGCTCGACGCAACC 637 P12TATTTTGTTTCGGATTCGTGTGCGCG 638 69 F669 GTTTCGAGTTCGAG 83 695 R12CGCTCGACGCAACC 637 P12 TATTTTGTTTCGGATTCGTGTGCGCG 638 70 F670TTTCGAGTTCGAGT 82 696 R12 CGCTCGACGCAACC 637 P12TATTTTGTTTCGGATTCGTGTGCGCG 638 71 F671 TTCGAGTTCGAGTT 81 697 R12CGCTCGACGCAACC 637 P12 TATTTTGTTTCGGATTCGTGTGCGCG 638 72 F672TCGAGTTCGAGTTT 80 698 R12 CGCTCGACGCAACC 637 P12TATTTTGTTTCGGATTCGTGTGCGCG 638 73 F673 CGAGTTCGAGTTTT 140 699 R13CAAAAACCGACTAC 700 P13 TTTGGTTGTAAGTAGCGGTTGGGA 701 74 F674GAGTTCGAGTTTTC 139 702 R13 CAAAAACCGACTAC 700 P13TTTGGTTGTAAGTAGCGGTTGGGA 701 75 F675 AGTTCGAGTTTTCG 138 703 R13CAAAAACCGACTAC 700 P13 TTTGGTTGTAAGTAGCGGTTGGGA 701 76 F676GTTCGAGTTTTCGA 137 704 R13 CAAAAACCGACTAC 700 P13TTTGGTTGTAAGTAGCGGTTGGGA 701 77 F677 TTCGAGTTTTCGAG 136 705 R13CAAAAACCGACTAC 700 P13 TTTGGTTGTAAGTAGCGGTTGGGA 701 78 F678TCGAGTTTTCGAGT 135 706 R13 CAAAAACCGACTAC 700 P13TTTGGTTGTAAGTAGCGGTTGGGA 701 79 F679 CGAGTTTTCGAGTT 134 707 R13CAAAAACCGACTAC 700 P13 TTTGGTTGTAAGTAGCGGTTGGGA 701 80 F680GAGTTTTCGAGTTT 133 708 R13 CAAAAACCGACTAC 700 P13TTTGGTTGTAAGTAGCGGTTGGGA 701 81 F681 AGTTTTCGAGTTTG 132 709 R13CAAAAACCGACTAC 700 P13 TTTGGTTGTAAGTAGCGGTTGGGA 701 82 F682GTTTTCGAGTTTGA 131 710 R13 CAAAAACCGACTAC 700 P13TTTGGTTGTAAGTAGCGGTTGGGA 701 83 F683 TTTTCGAGTTTGAG 130 711 R13CAAAAACCGACTAC 700 P13 TTTGGTTGTAAGTAGCGGTTGGGA 701 84 F684TTTCGAGTTTGAGT 129 712 R13 CAAAAACCGACTAC 700 P13TTTGGTTGTAAGTAGCGGTTGGGA 701 85 F685 TTCGAGTTTGAGTC 128 713 R13CAAAAACCGACTAC 700 P13 TTTGGTTGTAAGTAGCGGTTGGGA 701 86 F686TCGAGTTTGAGTCG 127 714 R13 CAAAAACCGACTAC 700 P13TTTGGTTGTAAGTAGCGGTTGGGA 701 87 F687 CGAGTTTGAGTCGT 126 715 R13CAAAAACCGACTAC 700 P13 TTTGGTTGTAAGTAGCGGTTGGGA 701 88 F688GAGTTTGAGTCGTA 125 716 R13 CAAAAACCGACTAC 700 P13TTTGGTTGTAAGTAGCGGTTGGGA 701 89 F689 AGTTTGAGTCGTAA 124 717 R13CAAAAACCGACTAC 700 P13 TTTGGTTGTAAGTAGCGGTTGGGA 701 90 F690GTTTGAGTCGTAAT 123 718 R13 CAAAAACCGACTAC 700 P13TTTGGTTGTAAGTAGCGGTTGGGA 701 91 F691 TTTGAGTCGTAATC 122 719 R13CAAAAACCGACTAC 700 P13 TTTGGTTGTAAGTAGCGGTTGGGA 701 92 F692TTGAGTCGTAATCG 121 720 R13 CAAAAACCGACTAC 700 P13TTTGGTTGTAAGTAGCGGTTGGGA 701 93 F693 TGAGTCGTAATCGT 120 721 R13CAAAAACCGACTAC 700 P13 TTTGGTTGTAAGTAGCGGTTGGGA 701 94 F694GAGTCGTAATCGTT 119 722 R13 CAAAAACCGACTAC 700 P13TTTGGTTGTAAGTAGCGGTTGGGA 701 95 F695 AGTCGTAATCGTTG 118 723 R13CAAAAACCGACTAC 700 P13 TTTGGTTGTAAGTAGCGGTTGGGA 701 96 F696GTCGTAATCGTTGC 117 724 R13 CAAAAACCGACTAC 700 P13TTTGGTTGTAAGTAGCGGTTGGGA 701 97 F697 TCGTAATCGTTGCG 116 725 R13CAAAAACCGACTAC 700 P13 TTTGGTTGTAAGTAGCGGTTGGGA 701 98 F698CGTAATCGTTGCGG 115 726 R13 CAAAAACCGACTAC 700 P13TTTGGTTGTAAGTAGCGGTTGGGA 701 99 F699 GTAATCGTTGCGGT 114 727 R13CAAAAACCGACTAC 700 P13 TTTGGTTGTAAGTAGCGGTTGGGA 701 00 F700TAATCGTTGCGGTA 113 728 R13 CAAAAACCGACTAC 700 P13TTTGGTTGTAAGTAGCGGTTGGGA 701 01 F701 AATCGTTGCGGTAT 112 729 R13CAAAAACCGACTAC 700 P13 TTTGGTTGTAAGTAGCGGTTGGGA 701 02 F702ATCGTTGCGGTATT 111 730 R13 CAAAAACCGACTAC 700 P13TTTGGTTGTAAGTAGCGGTTGGGA 701 03 F703 TCGTTGCGGTATTT 110 731 R13CAAAAACCGACTAC 700 P13 TTTGGTTGTAAGTAGCGGTTGGGA 701 04 F704CGTTGCGGTATTTT 109 732 R13 CAAAAACCGACTAC 700 P13TTTGGTTGTAAGTAGCGGTTGGGA 701 05 F705 GTTGCGGTATTTTG 108 733 R13CAAAAACCGACTAC 700 P13 TTTGGTTGTAAGTAGCGGTTGGGA 701 06 F706TTGCGGTATTTTGT 107 734 R13 CAAAAACCGACTAC 700 P13TTTGGTTGTAAGTAGCGGTTGGGA 701 07 F707 TGCGGTATTTTGTT 106 735 R13CAAAAACCGACTAC 700 P13 TTTGGTTGTAAGTAGCGGTTGGGA 701 08 F708GCGGTATTTTGTTT 105 736 R13 CAAAAACCGACTAC 700 P13TTTGGTTGTAAGTAGCGGTTGGGA 701 09 F709 CGGTATTTTGTTTC 104 737 R13CAAAAACCGACTAC 700 P13 TTTGGTTGTAAGTAGCGGTTGGGA 701 10 F710GGTATTTTGTTTCG 103 738 R13 CAAAAACCGACTAC 700 P13TTTGGTTGTAAGTAGCGGTTGGGA 701 11 F711 GTATTTTGTTTCGG 102 739 R13CAAAAACCGACTAC 700 P13 TTTGGTTGTAAGTAGCGGTTGGGA 701 12 F712TATTTTGTTTCGGA 101 740 R13 CAAAAACCGACTAC 700 P13TTTGGTTGTAAGTAGCGGTTGGGA 701 13 F713 ATTTTGTTTCGGAT 100 741 R13CAAAAACCGACTAC 700 P13 TTTGGTTGTAAGTAGCGGTTGGGA 701 14 F714TTTTGTTTCGGATT 99 742 R13 CAAAAACCGACTAC 700 P13TTTGGTTGTAAGTAGCGGTTGGGA 701 15 F715 TTTGTTTCGGATTC 98 743 R13CAAAAACCGACTAC 700 P13 TTTGGTTGTAAGTAGCGGTTGGGA 701 16 F716TTGTTTCGGATTCG 97 744 R13 CAAAAACCGACTAC 700 P13TTTGGTTGTAAGTAGCGGTTGGGA 701 17 F717 TGTTTCGGATTCGT 96 745 R13CAAAAACCGACTAC 700 P13 TTTGGTTGTAAGTAGCGGTTGGGA 701 18 F718GTTTCGGATTCGTG 95 746 R13 CAAAAACCGACTAC 700 P13TTTGGTTGTAAGTAGCGGTTGGGA 701 19 F719 TTTCGGATTCGTGT 94 747 R13CAAAAACCGACTAC 700 P13 TTTGGTTGTAAGTAGCGGTTGGGA 701 20 F720TTCGGATTCGTGTG 93 748 R13 CAAAAACCGACTAC 700 P13TTTGGTTGTAAGTAGCGGTTGGGA 701 21 F721 TCGGATTCGTGTGC 92 749 R13CAAAAACCGACTAC 700 P13 TTTGGTTGTAAGTAGCGGTTGGGA 701 22 F722CGGATTCGTGTGCG 91 750 R13 CAAAAACCGACTAC 700 P13TTTGGTTGTAAGTAGCGGTTGGGA 701 23 F723 GGATTCGTGTGCGC 90 751 R13CAAAAACCGACTAC 700 P13 TTTGGTTGTAAGTAGCGGTTGGGA 701 24 F724GATTCGTGTGCGCG 89 752 R13 CAAAAACCGACTAC 700 P13TTTGGTTGTAAGTAGCGGTTGGGA 701 25 F725 ATTCGTGTGCGCGG 88 753 R13CAAAAACCGACTAC 700 P13 TTTGGTTGTAAGTAGCGGTTGGGA 701 26 F726TTCGTGTGCGCGGG 87 754 R13 CAAAAACCGACTAC 700 P13TTTGGTTGTAAGTAGCGGTTGGGA 701 27 F727 TCGTGTGCGCGGGT 86 755 R13CAAAAACCGACTAC 700 P13 TTTGGTTGTAAGTAGCGGTTGGGA 701 28 F728CGTGTGCGCGGGTT 85 756 R13 CAAAAACCGACTAC 700 P13TTTGGTTGTAAGTAGCGGTTGGGA 701 29 F729 GTGTGCGCGGGTTG 84 757 R13CAAAAACCGACTAC 700 P13 TTTGGTTGTAAGTAGCGGTTGGGA 701 30 F730TGTGCGCGGGTTGC 83 758 R13 CAAAAACCGACTAC 700 P13TTTGGTTGTAAGTAGCGGTTGGGA 701 31 F731 GTGCGCGGGTTGCG 82 759 R13CAAAAACCGACTAC 700 P13 TTTGGTTGTAAGTAGCGGTTGGGA 701 32 F732TGCGCGGGTTGCGT 81 760 R13 CAAAAACCGACTAC 700 P13TTTGGTTGTAAGTAGCGGTTGGGA 701 33 F733 GCGCGGGTTGCGTC 80 761 R13CAAAAACCGACTAC 700 P13 TTTGGTTGTAAGTAGCGGTTGGGA 701 34 F734CGCGGGTTGCGTCG 140 762 R14 CCGCCGACACGCAA 763 P14TTTTGTTTATTTTGGGTTTGGTGGT 764 35 F735 GCGGGTTGCGTCGA 139 765 R14CCGCCGACACGCAA 763 P14 TTTTGTTTATTTTGGGTTTGGTGGT 764 36 F736CGGGTTGCGTCGAG 138 766 R14 CCGCCGACACGCAA 763 P14TTTTGTTTATTTTGGGTTTGGTGGT 764 37 F737 GGGTTGCGTCGAGC 137 767 R14CCGCCGACACGCAA 763 P14 TTTTGTTTATTTTGGGTTTGGTGGT 764 38 F738GGTTGCGTCGAGCG 136 768 R14 CCGCCGACACGCAA 763 P14TTTTGTTTATTTTGGGTTTGGTGGT 764 39 F739 GTTGCGTCGAGCGT 135 769 R14CCGCCGACACGCAA 763 P14 TTTTGTTTATTTTGGGTTTGGTGGT 764 40 F740TTGCGTCGAGCGTT 134 770 R14 CCGCCGACACGCAA 763 P14TTTTGTTTATTTTGGGTTTGGTGGT 764 41 F741 TGCGTCGAGCGTTG 133 771 R14CCGCCGACACGCAA 763 P14 TTTTGTTTATTTTGGGTTTGGTGGT 764 42 F742GCGTCGAGCGTTGG 132 772 R14 CCGCCGACACGCAA 763 P14TTTTGTTTATTTTGGGTTTGGTGGT 764 43 F743 CGTCGAGCGTTGGG 131 773 R14CCGCCGACACGCAA 763 P14 TTTTGTTTATTTTGGGTTTGGTGGT 764 44 F744GTCGAGCGTTGGGT 130 774 R14 CCGCCGACACGCAA 763 P14TTTTGTTTATTTTGGGTTTGGTGGT 764 45 F745 TCGAGCGTTGGGTA 129 775 R14CCGCCGACACGCAA 763 P14 TTTTGTTTATTTTGGGTTTGGTGGT 764 46 F746CGAGCGTTGGGTAG 128 776 R14 CCGCCGACACGCAA 763 P14TTTTGTTTATTTTGGGTTTGGTGGT 764 47 F747 GAGCGTTGGGTAGG 127 777 R14CCGCCGACACGCAA 763 P14 TTTTGTTTATTTTGGGTTTGGTGGT 764 48 F748AGCGTTGGGTAGGA 126 778 R14 CCGCCGACACGCAA 763 P14TTTTGTTTATTTTGGGTTTGGTGGT 764 49 F749 GCGTTGGGTAGGAG 125 779 R14CCGCCGACACGCAA 763 P14 TTTTGTTTATTTTGGGTTTGGTGGT 764 50 F750CGTTGGGTAGGAGG 124 780 R14 CCGCCGACACGCAA 763 P14TTTTGTTTATTTTGGGTTTGGTGGT 764 51 F751 GTTGGGTAGGAGGT 123 781 R14CCGCCGACACGCAA 763 P14 TTTTGTTTATTTTGGGTTTGGTGGT 764 52 F752TTGGGTAGGAGGTT 122 782 R14 CCGCCGACACGCAA 763 P14TTTTGTTTATTTTGGGTTTGGTGGT 764 53 F753 TGGGTAGGAGGTTT 121 783 R14CCGCCGACACGCAA 763 P14 TTTTGTTTATTTTGGGTTTGGTGGT 764 54 F754GGGTAGGAGGTTTC 120 784 R14 CCGCCGACACGCAA 763 P14TTTTGTTTATTTTGGGTTTGGTGGT 764 55 F755 GGTAGGAGGTTTCG 119 785 R14CCGCCGACACGCAA 763 P14 TTTTGTTTATTTTGGGTTTGGTGGT 764 56 F756GTAGGAGGTTTCGT 118 786 R14 CCGCCGACACGCAA 763 P14TTTTGTTTATTTTGGGTTTGGTGGT 764 57 F757 TAGGAGGTTTCGTT 117 787 R14CCGCCGACACGCAA 763 P14 TTTTGTTTATTTTGGGTTTGGTGGT 764 58 F758AGGAGGTTTCGTTT 116 788 R14 CCGCCGACACGCAA 763 P14TTTTGTTTATTTTGGGTTTGGTGGT 764 59 F759 GGAGGTTTCGTTTT 115 789 R14CCGCCGACACGCAA 763 P14 TTTTGTTTATTTTGGGTTTGGTGGT 764 60 F760GAGGTTTCGTTTTG 114 790 R14 CCGCCGACACGCAA 763 P14TTTTGTTTATTTTGGGTTTGGTGGT 764 61 F761 AGGTTTCGTTTTGT 113 791 R14CCGCCGACACGCAA 763 P14 TTTTGTTTATTTTGGGTTTGGTGGT 764 62 F762GGTTTCGTTTTGTT 112 792 R14 CCGCCGACACGCAA 763 P14TTTTGTTTATTTTGGGTTTGGTGGT 764 63 F763 GTTTCGTTTTGTTT 111 793 R14CCGCCGACACGCAA 763 P14 TTTTGTTTATTTTGGGTTTGGTGGT 764 64 F764TTTCGTTTTGTTTT 110 794 R14 CCGCCGACACGCAA 763 P14TTTTGTTTATTTTGGGTTTGGTGGT 764 65 F765 TTCGTTTTGTTTTG 109 795 R14CCGCCGACACGCAA 763 P14 TTTTGTTTATTTTGGGTTTGGTGGT 764 66 F766TCGTTTTGTTTTGG 108 796 R14 CCGCCGACACGCAA 763 P14TTTTGTTTATTTTGGGTTTGGTGGT 764 67 F767 CGTTTTGTTTTGGT 107 797 R14CCGCCGACACGCAA 763 P14 TTTTGTTTATTTTGGGTTTGGTGGT 764 68 F768GTTTTGTTTTGGTT 106 798 R14 CCGCCGACACGCAA 763 P14TTTTGTTTATTTTGGGTTTGGTGGT 764 69 F769 TTTTGTTTTGGTTG 105 799 R14CCGCCGACACGCAA 763 P14 TTTTGTTTATTTTGGGTTTGGTGGT 764 70 F770TTTGTTTTGGTTGT 104 800 R14 CCGCCGACACGCAA 763 P14TTTTGTTTATTTTGGGTTTGGTGGT 764 71 F771 TTGTTTTGGTTGTA 103 801 R14CCGCCGACACGCAA 763 P14 TTTTGTTTATTTTGGGTTTGGTGGT 764 72 F772TGTTTTGGTTGTAA 102 802 R14 CCGCCGACACGCAA 763 P14TTTTGTTTATTTTGGGTTTGGTGGT 764 73 F773 GTTTTGGTTGTAAG 101 803 R14CCGCCGACACGCAA 763 P14 TTTTGTTTATTTTGGGTTTGGTGGT 764 74 F774TTTTGGTTGTAAGT 100 804 R14 CCGCCGACACGCAA 763 P14TTTTGTTTATTTTGGGTTTGGTGGT 764 75 F775 TTTGGTTGTAAGTA 99 805 R14CCGCCGACACGCAA 763 P14 TTTTGTTTATTTTGGGTTTGGTGGT 764 76 F776TTGGTTGTAAGTAG 98 806 R14 CCGCCGACACGCAA 763 P14TTTTGTTTATTTTGGGTTTGGTGGT 764 77 F777 TGGTTGTAAGTAGC 97 807 R14CCGCCGACACGCAA 763 P14 TTTTGTTTATTTTGGGTTTGGTGGT 764 78 F778GGTTGTAAGTAGCG 96 808 R14 CCGCCGACACGCAA 763 P14TTTTGTTTATTTTGGGTTTGGTGGT 764 79 F779 GTTGTAAGTAGCGG 95 809 R14CCGCCGACACGCAA 763 P14 TTTTGTTTATTTTGGGTTTGGTGGT 764 80 F780TTGTAAGTAGCGGT 94 810 R14 CCGCCGACACGCAA 763 P14TTTTGTTTATTTTGGGTTTGGTGGT 764 81 F781 TGTAAGTAGCGGTT 93 811 R14CCGCCGACACGCAA 763 P14 TTTTGTTTATTTTGGGTTTGGTGGT 764 82 F782GTAAGTAGCGGTTG 92 812 R14 CCGCCGACACGCAA 763 P14TTTTGTTTATTTTGGGTTTGGTGGT 764 83 F783 TAAGTAGCGGTTGG 91 813 R14CCGCCGACACGCAA 763 P14 TTTTGTTTATTTTGGGTTTGGTGGT 764 84 F784AAGTAGCGGTTGGG 90 814 R14 CCGCCGACACGCAA 763 P14TTTTGTTTATTTTGGGTTTGGTGGT 764 85 F785 AGTAGCGGTTGGGA 89 815 R14CCGCCGACACGCAA 763 P14 TTTTGTTTATTTTGGGTTTGGTGGT 764 86 F786GTAGCGGTTGGGAG 88 816 R14 CCGCCGACACGCAA 763 P14TTTTGTTTATTTTGGGTTTGGTGGT 764 87 F787 TAGCGGTTGGGAGT 87 817 R14CCGCCGACACGCAA 763 P14 TTTTGTTTATTTTGGGTTTGGTGGT 764 88 F788AGCGGTTGGGAGTA 86 818 R14 CCGCCGACACGCAA 763 P14TTTTGTTTATTTTGGGTTTGGTGGT 764 89 F789 GCGGTTGGGAGTAG 85 819 R14CCGCCGACACGCAA 763 P14 TTTTGTTTATTTTGGGTTTGGTGGT 764 90 F790CGGTTGGGAGTAGT 84 820 R14 CCGCCGACACGCAA 763 P14TTTTGTTTATTTTGGGTTTGGTGGT 764 91 F791 GGTTGGGAGTAGTC 83 821 R14CCGCCGACACGCAA 763 P14 TTTTGTTTATTTTGGGTTTGGTGGT 764 92 F792GTTGGGAGTAGTCG 82 822 R14 CCGCCGACACGCAA 763 P14TTTTGTTTATTTTGGGTTTGGTGGT 764 93 F793 TTGGGAGTAGTCGG 81 823 R14CCGCCGACACGCAA 763 P14 TTTTGTTTATTTTGGGTTTGGTGGT 764 94 F794TGGGAGTAGTCGGT 80 824 R14 CCGCCGACACGCAA 763 P14TTTTGTTTATTTTGGGTTTGGTGGT 764 95 F795 GGGAGTAGTCGGTT 140 825 R15TCTCGTAACTTCAA 826 P15 GGATGCGCGCGTCGTTTAGGGTGT 827 96 F796GGAGTAGTCGGTTT 139 828 R15 TCTCGTAACTTCAA 826 P15GGATGCGCGCGTCGTTTAGGGTGT 827 97 F797 GAGTAGTCGGTTTT 138 829 R15TCTCGTAACTTCAA 826 P15 GGATGCGCGCGTCGTTTAGGGTGT 827 98 F798AGTAGTCGGTTTTT 137 830 R15 TCTCGTAACTTCAA 826 P15GGATGCGCGCGTCGTTTAGGGTGT 827 99 F799 GTAGTCGGTTTTTG 136 831 R15TCTCGTAACTTCAA 826 P15 GGATGCGCGCGTCGTTTAGGGTGT 827 00 F800TAGTCGGTTTTTGG 135 832 R15 TCTCGTAACTTCAA 826 P15GGATGCGCGCGTCGTTTAGGGTGT 827 01 F801 AGTCGGTTTTTGGG 134 833 R15TCTCGTAACTTCAA 826 P15 GGATGCGCGCGTCGTTTAGGGTGT 827 02 F802GTCGGTTTTTGGGG 133 834 R15 TCTCGTAACTTCAA 826 P15GGATGCGCGCGTCGTTTAGGGTGT 827 03 F803 TCGGTTTTTGGGGA 132 835 R15TCTCGTAACTTCAA 826 P15 GGATGCGCGCGTCGTTTAGGGTGT 827 04 F804CGGTTTTTGGGGAA 131 836 R15 TCTCGTAACTTCAA 826 P15GGATGCGCGCGTCGTTTAGGGTGT 827 05 F805 GGTTTTTGGGGAAT 130 837 R15TCTCGTAACTTCAA 826 P15 GGATGCGCGCGTCGTTTAGGGTGT 827 06 F806GTTTTTGGGGAATA 129 838 R15 TCTCGTAACTTCAA 826 P15GGATGCGCGCGTCGTTTAGGGTGT 827 07 F807 TTTTTGGGGAATAT 128 839 R15TCTCGTAACTTCAA 826 P15 GGATGCGCGCGTCGTTTAGGGTGT 827 08 F808GTAGAAATTAATAAGTGAGAGGGC 124 840 R16 ACGACTCAAACTCGAAAACTCG 841 P16TTCGGGGCGTAGTTGCGGGCGG 842

FIG. 1 shows a graph diagram showing a detection rate at which SDC2 genemethylation is detected in various specimens using 12 sets among 808sets of primers and probes as an example used in the method according tothe present disclosure. There occurs an amplification curve in themethylated DNA, there does not occur an amplification curve in a groupusing the non-methylated DNA and distilled water (D.W) as a template.

TABLE 2 Primer and probe sequences for detection of SDC2 genemethylation Set of primer qMSP CT value and probe Methylated DNANon-methylated DNA 1 24.6 N.D 2 24.4 N.D 3 24.2 N.D 4 25.1 N.D 5 24.9N.D 6 25.9 N.D 7 27.6 N.D 8 24.3 N.D 9 24.3 N.D 10 23.9 N.D 11 25.3 N.D12 26.4 N.D 13 27.4 N.D 14 26.3 N.D 15 25.2 N.D 16 24.3 N.D 17 24.3 N.D18 28.3 N.D 19 25.3 N.D 20 26.4 N.D 21 27.4 N.D 22 26.3 N.D 23 25.2 N.D24 25.7 N.D 25 27.6 N.D 26 27.8 N.D 27 29.3 N.D 28 25.4 N.D 29 25.7 N.D30 27.4 N.D 31 24.3 N.D 32 28.3 N.D 33 25.3 N.D 34 26.4 N.D 35 27.4 N.D36 26.3 N.D 37 25.2 N.D 38 25.7 N.D 39 27.6 N.D 40 27.8 N.D 41 29.3 N.D42 25.4 N.D 43 25.7 N.D 44 27.4 N.D 45 28.2 N.D 46 27.2 N.D 47 24.2 N.D48 27.9 N.D 49 28.6 N.D 50 28.4 N.D 51 24.4 N.D 52 24.2 N.D 53 25.1 N.D54 24.9 N.D 55 25.9 N.D 56 27.6 N.D 57 24.3 N.D 58 24.3 N.D 59 25.7 N.D60 27.4 N.D 61 28.2 N.D 62 27.2 N.D 63 24.2 N.D 64 27.9 N.D 65 28.6 N.D66 28.4 N.D 67 24.4 N.D 68 24.2 N.D 69 25.1 N.D 70 24.9 N.D 71 25.9 N.D72 27.6 N.D 73 24.3 N.D 74 24.3 N.D 75 28.3 N.D 76 25.3 N.D 77 26.4 N.D78 27.4 N.D 79 26.3 N.D 80 25.2 N.D 81 25.7 N.D 82 28.2 N.D 83 27.2 N.D84 24.2 N.D 85 27.9 N.D 86 28.6 N.D 87 28.4 N.D 88 24.4 N.D 89 24.2 N.D90 25.1 N.D 91 24.9 N.D 92 25.9 N.D 93 27.6 N.D 94 24.3 N.D 95 24.2 N.D96 25.2 N.D 97 25.7 N.D 98 27.6 N.D 99 27.8 N.D 100 29.1 N.D 101 25.4N.D 102 25.7 N.D 103 27.4 N.D 104 28.2 N.D 105 27.2 N.D 106 24.2 N.D 10727.9 N.D 108 28.6 N.D 109 28.4 N.D 110 24.4 N.D 111 24.2 N.D 112 25.1N.D 113 24.9 N.D 114 25.9 N.D 115 27.6 N.D 116 24.3 N.D 117 24.3 N.D 11828.3 N.D 119 25.3 N.D 120 26.4 N.D 121 27.8 N.D 122 29.3 N.D 123 25.4N.D 124 25.7 N.D 125 27.4 N.D 126 28.2 N.D 127 27.2 N.D 128 24.2 N.D 12927.9 N.D 130 28.6 N.D 131 28.4 N.D 132 24.4 N.D 133 24.2 N.D 134 25.1N.D 135 24.9 N.D 136 28.4 N.D 137 24.4 N.D 138 24.2 N.D 139 25.1 N.D 14024.9 N.D 141 25.9 N.D 142 27.6 N.D 143 24.3 N.D 144 24.3 N.D 145 28.3N.D 146 25.3 N.D 147 26.4 N.D 148 27.4 N.D 149 26.3 N.D 150 25.2 N.D 15125.7 N.D 152 27.6 N.D 153 27.8 N.D 154 29.3 N.D 155 25.4 N.D 156 27.4N.D 157 26.3 N.D 158 25.2 N.D 159 25.7 N.D 160 27.6 N.D 161 27.8 N.D 16229.3 N.D 163 25.4 N.D 164 25.7 N.D 165 27.4 N.D 166 28.2 N.D 167 27.2N.D 168 24.2 N.D 169 27.9 N.D 170 28.6 N.D 171 28.4 N.D 172 24.4 N.D 17324.2 N.D 174 25.1 N.D 175 24.9 N.D 176 25.9 N.D 177 27.6 N.D 178 24.3N.D 179 24.2 N.D 180 27.6 N.D 181 27.8 N.D 182 29.3 N.D 183 25.4 N.D 18425.7 N.D 185 27.4 N.D 186 28.2 N.D 187 27.2 N.D 188 24.2 N.D 189 27.9N.D 190 28.6 N.D 191 28.4 N.D 192 24.4 N.D 193 24.2 N.D 194 25.1 N.D 19524.9 N.D 196 25.9 N.D 197 27.6 N.D 198 24.3 N.D 199 24.3 N.D 200 28.4N.D 201 28.2 N.D 202 27.2 N.D 203 24.2 N.D 204 27.9 N.D 205 28.6 N.D 20628.4 N.D 207 24.4 N.D 208 24.2 N.D 209 25.1 N.D 210 24.9 N.D 211 25.9N.D 212 27.6 N.D 213 24.3 N.D 214 24.3 N.D 215 28.3 N.D 216 25.3 N.D 21726.4 N.D 218 27.4 N.D 219 26.3 N.D 220 25.2 N.D 221 25.7 N.D 222 27.6N.D 223 27.8 N.D 224 29.3 N.D 225 24.4 N.D 226 24.2 N.D 227 25.1 N.D 22824.9 N.D 229 25.9 N.D 230 27.6 N.D 231 24.3 N.D 232 24.3 N.D 233 28.3N.D 234 25.3 N.D 235 26.4 N.D 236 27.4 N.D 237 26.3 N.D 238 25.2 N.D 23925.7 N.D 240 27.6 N.D 241 27.8 N.D 242 29.3 N.D 243 25.4 N.D 244 25.7N.D 245 27.4 N.D 246 28.2 N.D 247 27.2 N.D 248 24.2 N.D 249 25.3 N.D 25026.3 N.D 251 25.9 N.D 252 26.4 N.D 253 27.4 N.D 254 26.3 N.D 255 25.2N.D 256 25.7 N.D 257 27.6 N.D 258 27.8 N.D 259 29.3 N.D 260 25.4 N.D 26125.7 N.D 262 27.4 N.D 263 27.4 N.D 264 26.3 N.D 265 25.2 N.D 266 25.7N.D 267 27.6 N.D 268 27.8 N.D 269 29.3 N.D 270 25.4 N.D 271 25.7 N.D 27227.4 N.D 273 26.3 N.D 274 25.2 N.D 275 25.7 N.D 276 27.6 N.D 277 27.8N.D 278 29.3 N.D 279 25.4 N.D 280 25.7 N.D 281 27.4 N.D 282 28.2 N.D 28327.2 N.D 284 27.4 N.D 285 26.3 N.D 286 25.2 N.D 287 25.7 N.D 288 27.6N.D 289 27.8 N.D 290 29.3 N.D 291 27.3 N.D 292 25.2 N.D 293 26.2 N.D 29427.4 N.D 295 26.3 N.D 296 25.2 N.D 297 25.7 N.D 298 27.6 N.D 299 27.8N.D 300 24.0 N.D 301 26.3 N.D 302 25.2 N.D 303 25.7 N.D 304 27.6 N.D 30527.8 N.D 306 29.3 N.D 307 25.4 N.D 308 25.7 N.D 309 27.4 N.D 310 24.2N.D 311 27.9 N.D 312 28.6 N.D 313 28.4 N.D 314 24.4 N.D 315 24.2 N.D 31625.1 N.D 317 24.9 N.D 318 25.9 N.D 319 27.6 N.D 320 24.3 N.D 321 24.3N.D 322 28.3 N.D 323 25.3 N.D 324 26.4 N.D 325 27.4 N.D 326 26.3 N.D 32725.2 N.D 328 27.6 N.D 329 27.8 N.D 330 29.3 N.D 331 25.4 N.D 332 27.4N.D 333 26.3 N.D 334 25.2 N.D 335 25.7 N.D 336 27.6 N.D 337 27.8 N.D 33829.3 N.D 339 24.3 N.D 340 25.2 N.D 341 26.8 N.D 342 27.4 N.D 343 28.2N.D 344 27.2 N.D 345 24.2 N.D 346 27.9 N.D 347 28.6 N.D 348 28.4 N.D 34924.4 N.D 350 24.2 N.D 351 25.1 N.D 352 27.4 N.D 353 26.3 N.D 354 25.2N.D 355 25.7 N.D 356 27.6 N.D 357 27.9 N.D 358 28.6 N.D 359 28.4 N.D 36024.4 N.D 361 24.2 N.D 362 25.1 N.D 363 24.9 N.D 364 25.9 N.D 365 27.6N.D 366 24.3 N.D 367 25.8 N.D 368 26.1 N.D 369 27.7 N.D 370 25.3 N.D 37127.9 N.D 372 28.6 N.D 373 28.4 N.D 374 24.4 N.D 375 24.2 N.D 376 25.1N.D 377 24.9 N.D 378 25.9 N.D 379 27.6 N.D 380 24.3 N.D 381 27.4 N.D 38226.3 N.D 383 25.2 N.D 384 25.7 N.D 385 27.6 N.D 386 27.8 N.D 387 29.3N.D 388 25.1 N.D 389 26.4 N.D 390 27.4 N.D 391 26.3 N.D 392 25.2 N.D 39325.7 N.D 394 27.6 N.D 395 27.8 N.D 396 29.3 N.D 397 25.4 N.D 398 25.7N.D 399 27.4 N.D 400 24.4 N.D 401 27.2 N.D 402 24.2 N.D 403 27.9 N.D 40427.6 N.D 405 24.2 N.D 406 27.9 N.D 407 28.6 N.D 408 28.4 N.D 409 24.4N.D 410 24.2 N.D 411 25.1 N.D 412 24.9 N.D 413 25.9 N.D 414 27.6 N.D 41524.3 N.D 416 24.3 N.D 417 28.3 N.D 418 25.3 N.D 419 27.4 N.D 420 26.3N.D 421 25.2 N.D 422 25.7 N.D 423 27.6 N.D 424 27.8 N.D 425 29.3 N.D 42627.9 N.D 427 28.6 N.D 428 28.4 N.D 429 24.4 N.D 430 24.2 N.D 431 25.1N.D 432 24.9 N.D 433 25.9 N.D 434 27.6 N.D 435 24.3 N.D 436 27.4 N.D 43726.3 N.D 438 25.2 N.D 439 25.7 N.D 440 27.6 N.D 441 27.8 N.D 442 29.3N.D 443 25.4 N.D 444 27.4 N.D 445 26.3 N.D 446 25.2 N.D 447 25.7 N.D 44827.6 N.D 449 27.8 N.D 450 29.3 N.D 451 28.2 N.D 452 27.2 N.D 453 24.2N.D 454 27.9 N.D 455 28.6 N.D 456 28.4 N.D 457 24.4 N.D 458 24.2 N.D 45925.1 N.D 460 24.9 N.D 461 24.2 N.D 462 27.9 N.D 463 28.6 N.D 464 28.4N.D 465 24.4 N.D 466 24.2 N.D 467 25.1 N.D 468 24.9 N.D 469 25.9 N.D 47027.6 N.D 471 24.3 N.D 472 24.3 N.D 473 28.3 N.D 474 25.3 N.D 475 26.4N.D 476 27.4 N.D 477 26.3 N.D 478 25.2 N.D 479 27.8 N.D 480 29.3 N.D 48125.4 N.D 482 25.7 N.D 483 27.4 N.D 484 28.2 N.D 485 27.4 N.D 486 26.3N.D 487 25.2 N.D 488 25.7 N.D 489 27.6 N.D 490 27.8 N.D 491 29.3 N.D 49225.4 N.D 493 27.4 N.D 494 26.3 N.D 495 25.2 N.D 496 25.7 N.D 497 27.6N.D 498 27.8 N.D 499 29.3 N.D 500 25.4 N.D 501 27.9 N.D 502 28.6 N.D 50328.4 N.D 504 24.4 N.D 505 24.2 N.D 506 25.1 N.D 507 24.9 N.D 508 25.9N.D 509 27.6 N.D 510 24.3 N.D 511 25.5 N.D 512 27.8 N.D 513 28.2 N.D 51426.1 N.D 515 27.4 N.D 516 26.3 N.D 517 25.2 N.D 518 25.7 N.D 519 27.6N.D 520 27.8 N.D 521 29.3 N.D 522 26.2 N.D 523 25.3 N.D 524 28.2 N.D 52527.4 N.D 526 28.2 N.D 527 27.2 N.D 528 24.2 N.D 529 27.9 N.D 530 28.6N.D 531 28.4 N.D 532 24.4 N.D 533 24.2 N.D 534 25.1 N.D 535 24.9 N.D 53625.9 N.D 537 27.6 N.D 538 25.2 N.D 539 25.7 N.D 540 27.6 N.D 541 27.4N.D 542 26.3 N.D 543 25.2 N.D 544 25.7 N.D 545 27.6 N.D 546 27.8 N.D 54729.3 N.D 548 25.4 N.D 549 25.7 N.D 550 27.4 N.D 551 28.2 N.D 552 27.2N.D 553 24.2 N.D 554 27.4 N.D 555 26.3 N.D 556 25.2 N.D 557 25.7 N.D 55827.6 N.D 559 27.8 N.D 560 29.3 N.D 561 28.4 N.D 562 24.4 N.D 563 24.2N.D 564 25.1 N.D 565 24.9 N.D 566 25.9 N.D 567 27.6 N.D 568 24.3 N.D 56924.3 N.D 570 28.3 N.D 571 25.3 N.D 572 26.4 N.D 573 27.4 N.D 574 26.3N.D 575 25.2 N.D 576 25.7 N.D 577 27.6 N.D 578 27.8 N.D 579 29.3 N.D 58025.4 N.D 581 25.7 N.D 582 27.4 N.D 583 28.2 N.D 584 27.2 N.D 585 24.2N.D 586 24.2 N.D 587 26.3 N.D 588 25.2 N.D 589 25.7 N.D 590 27.6 N.D 59127.8 N.D 592 29.3 N.D 593 27.4 N.D 594 28.2 N.D 595 27.2 N.D 596 24.2N.D 597 27.9 N.D 598 28.6 N.D 599 28.4 N.D 600 27.0 N.D 601 24.2 N.D 60225.1 N.D 603 24.9 N.D 604 25.9 N.D 605 24.2 N.D 606 27.9 N.D 607 28.6N.D 608 28.4 N.D 609 24.4 N.D 610 24.2 N.D 611 25.1 N.D 612 24.9 N.D 61325.9 N.D 614 27.6 N.D 615 24.3 N.D 616 24.3 N.D 617 28.3 N.D 618 25.3N.D 619 26.4 N.D 620 27.4 N.D 621 26.3 N.D 622 25.2 N.D 623 26.3 N.D 62425.2 N.D 625 25.7 N.D 626 27.6 N.D 627 27.8 N.D 628 29.3 N.D 629 25.4N.D 630 25.7 N.D 631 27.4 N.D 632 28.2 N.D 633 27.2 N.D 634 24.2 N.D 63527.4 N.D 636 26.3 N.D 637 25.2 N.D 638 25.7 N.D 639 27.6 N.D 640 27.8N.D 641 29.3 N.D 642 24.2 N.D 643 27.9 N.D 644 28.6 N.D 645 28.4 N.D 64624.4 N.D 647 24.2 N.D 648 25.1 N.D 649 24.9 N.D 650 25.9 N.D 651 27.6N.D 652 26.1 N.D 653 24.8 N.D 654 25.5 N.D 655 25.7 N.D 656 24.9 N.D 65724.2 N.D 658 25.5 N.D 659 25.4 N.D 660 26.8 N.D 661 26.9 N.D 662 24.7N.D 663 25.5 N.D 664 27.4 N.D 665 26.3 N.D 666 25.2 N.D 667 25.7 N.D 66827.6 N.D 669 27.8 N.D 670 27.9 N.D 671 28.6 N.D 672 28.4 N.D 673 24.4N.D 674 24.2 N.D 675 25.1 N.D 676 24.9 N.D 677 25.9 N.D 678 27.6 N.D 67924.3 N.D 680 27.4 N.D 681 26.3 N.D 682 25.2 N.D 683 25.7 N.D 684 27.6N.D 685 27.8 N.D 686 29.3 N.D 687 25.4 N.D 688 25.7 N.D 689 24.2 N.D 69025.7 N.D 691 25.6 N.D 692 24.7 N.D 693 27.4 N.D 694 26.3 N.D 695 25.2N.D 696 25.7 N.D 697 27.6 N.D 698 27.9 N.D 699 28.6 N.D 700 28.4 N.D 70124.4 N.D 702 24.2 N.D 703 25.1 N.D 704 24.9 N.D 705 25.9 N.D 706 27.6N.D 707 24.3 N.D 708 29.3 N.D 709 25.4 N.D 710 25.7 N.D 711 27.4 N.D 71228.2 N.D 713 27.2 N.D 714 24.2 N.D 715 27.9 N.D 716 28.6 N.D 717 28.4N.D 718 24.4 N.D 719 24.2 N.D 720 25.1 N.D 721 24.9 N.D 722 25.9 N.D 72327.6 N.D 724 24.3 N.D 725 24.3 N.D 726 28.3 N.D 727 25.3 N.D 728 26.4N.D 729 27.4 N.D 730 26.3 N.D 731 25.2 N.D 732 27.9 N.D 733 28.6 N.D 73428.4 N.D 735 24.4 N.D 736 29.5 N.D 737 25.1 N.D 738 24.9 N.D 739 25.9N.D 740 27.6 N.D 741 25.4 N.D 742 26.3 N.D 743 27.8 N.D 744 25.8 N.D 74524.1 N.D 746 24.2 N.D 747 27.9 N.D 748 27.9 N.D 749 28.6 N.D 750 28.4N.D 751 24.4 N.D 752 24.2 N.D 753 25.1 N.D 754 24.9 N.D 755 25.9 N.D 75627.6 N.D 757 24.3 N.D 758 28.3 N.D 759 25.3 N.D 760 24.5 N.D 761 27.4N.D 762 26.3 N.D 763 25.2 N.D 764 27.6 N.D 765 27.8 N.D 766 29.3 N.D 76725.4 N.D 768 27.4 N.D 769 26.3 N.D 770 25.2 N.D 771 25.7 N.D 772 27.6N.D 773 27.9 N.D 774 28.6 N.D 775 28.4 N.D 776 24.4 N.D 777 24.2 N.D 77825.1 N.D 779 24.9 N.D 780 25.9 N.D 781 27.6 N.D 782 24.3 N.D 783 25.2N.D 784 27.9 N.D 785 28.6 N.D 786 28.4 N.D 787 24.4 N.D 788 24.2 N.D 78925.1 N.D 790 24.9 N.D 791 25.9 N.D 792 27.6 N.D 793 24.3 N.D 794 24.2N.D 795 27.9 N.D 796 28.6 N.D 797 28.4 N.D 798 24.4 N.D 799 24.2 N.D 80024.2 N.D 801 24.9 N.D 802 25.9 N.D 803 27.6 N.D 804 25.4 N.D 805 25.3N.D 806 26.2 N.D 807 27.1 N.D 808 23.7 N.D N.D: Not Detected

Example 2: Detection of SDC2 Gene Methylation in DNA of Cell Line, Fecesand Serum Specimens

The abilities of the above-described primers and probes to detect SDC2gene methylation in various specimens were examined. To this end, setNo. 808 that showed the lowest C_(T) value of 23.7 in methylated DNA inthe methylation measurement experiments performed using methylated andunmethylated DNAs was selected by way of example. In order to examinewhether or not SDC2 gene methylation would be detected in variousspecimens, varying amounts (from 20 ng to 0.01 ng) of the genomic DNA ofSDC2-methylated colorectal cancer cell line HCT116 (ATCC, CCL247) werespiked to 20 ng of the genomic DNA of SDC2 gene-unmethylated cell lineMRC-5 (Korean Cell Line Bank, KCLB No. 10171), DNA isolated from 1.0 mLof SDC2 gene-unmethylated human serum, and 2.0 μg of SDC2gene-unmethylated human feces DNA, and then qMSP was performedrepeatedly 24 times in the same manner as described in Example 1,thereby determining the detection rate of SDC2 gene methylation (FIG.2). FIG. 3 shows the detection rate of SDC2 gene methylation in each ofthe specimens. The cell line DNA showed a detection rate of 100% in agenomic DNA amount ranging from 20 ng to 0.05 ng, and showed detectionrates of 96% and 88% at 0.02 g and 0.01 ng, respectively. The feces DNAshowed a detection rate of 100% in a genomic DNA amount ranging from 20ng to 0.1 ng, and showed detection rates of 96%, 92% and 54% at 0.05 ng,0.02 ng and 0.01 ng, respectively. The serum DNA showed a detection rateof 100% in a genomic DNA amount ranging from 20 ng to 0.1 ng, and showeddetection rates of 96%, 71% and 33% at 0.05 ng, 0.02 ng and 0.01 ng,respectively. In conclusion, it was shown that SDC2 gene methylationcould be detected in various specimens, including cell lines, feces andserum.

TABLE 3 Detection rates of SDC2 gene methylation in various specimensHCT116 genomic Detection rates (%) DNA (ng) Cell line Feces Serum 100100 100 100 2 100 100 100 0.5 100 100 100 0.2 100 100 100 0.1 100 100100 0.05 100 96 96 0.02 96 92 71 0.01 88 54 33 0 0 0 0

Example 3: Detection of SDC2 Gene Methylation in DNA of Clinical Fecesand Serum Samples

The abilities of the primers and probes to detect SDC2 gene methylationin various specimens were examined again. To this end, set No. 808 thatshowed the lowest C_(T) value of 23.7 in methylated DNA in themethylation measurement experiments performed using methylated andunmethylated DNAs was selected by way of example. In order to examinewhether or not SDC2 gene methylation would be detected in clinical fecessamples, genomic DNA was isolated from each of 47 colorectal cancerpatients and 16 normal persons. 2.0 μg of the isolated genomic DNA wasconverted with bisulfite by use of the EZ DNA Methylation Gold kit (ZymoResearch) according to the manufacturer's instructions, and eluted with10 μL of distilled water. Using these genomic DNAs, methylation-specificreal-time PCR (qMSP) was performed using the 808 sets ofmethylation-specific primers and probes. The qMSP was performed using aRotor-Gene Q PCR system (Qiagen). Specifically, a total of 20 μL of PCRreaction solution (containing 2 μl of template DNA; 4 μL of 5× AptaTaqDNA Master (Roche Diagnostics); 2 μL (2 pmole/μL) of PCR primer, 2 μL (2pmole/μL) of TaqMan probe; and 10 μL of D.W.) was prepared and subjectedto PCR under the following conditions: treatment at 95° C. for 5 min,and then 40 cycles, each consisting of 15 sec at 95° C. and 1 min at anannealing temperature of 60° C. Whether or not a PCR amplificationproduct would be produced was determined by measuring the cyclethreshold (C_(T)) value.

Using the C_(T) value, sensitivity and specificity for diagnosis ofcolorectal cancer were evaluated by ROC analysis (MedCalc program,Belgium). As a result, it was shown that sensitivity for diagnosis ofcolorectal cancer was 89.4% (42/47), and specificity for diagnosis ofcolorectal cancer was 93.8% (1/16), indicating that sensitivity andspecificity for diagnosis of colorectal cancer were excellent (FIG. 2).

In addition, In order to examine whether or not SDC2 gene methylationwould be detected in clinical serum samples from colorectal cancerpatients, genomic DNA was isolated from each of 13 colorectal cancerpatients and 6 normal persons. The isolated genomic DNA was convertedwith bisulfite by use of the EZ DNA Methylation Gold kit (Zymo Research)according to the manufacturer's instructions, and eluted with 10 μL ofdistilled water. Using these genomic DNAs, methylation of SDC2 gene wasmeasured in the same manner as in the above Example using the 808 setsof methylation-specific primers and probes. Using the C_(T) value,sensitivity and specificity for diagnosis of colorectal cancer wereevaluated by ROC analysis (MedCalc program, Belgium). As a result, itwas shown that sensitivity for diagnosis of colorectal cancer was 84.6%(11/13), and specificity for diagnosis of colorectal cancer was 100%(0/6), indicating that sensitivity and specificity for diagnosis ofcolorectal cancer were excellent (FIG. 3).

What is claimed is:
 1. A method for detecting CpG methylation of SDC2 (Syndecan 2) gene, the method comprising the steps of: (a) isolating genomic DNA from a clinical sample; (b) treating the genomic DNA from step (a) with bisulfite; and (c) measuring methylation of a CpG of the SDC2 gene in the genomic DNA treated with bisulfite according the step (b) by using a primer pair comprising a primer comprising the sequence of SEQ ID NO: 574; and a primer comprising the sequence of any one of SEQ ID NOs: 573, 577 to 594, and 612 to 635, to amplify a methylated CpG of the bisulfite-treated SDC2 gene.
 2. The method according to claim 1, wherein step (c) is performed by a method selected from the group consisting of PCR, methylation specific PCR, real-time methylation specific PCR, PCR using a methylated DNA-specific binding protein, quantitative PCR, pyrosequencing, and bisulfite sequencing.
 3. The method according to claim 1, wherein step (c) comprises measuring a CpG methylation of a regulatory region or intron region of SDC2 gene in the clinical sample.
 4. The method of claim 1, wherein the primer(s) of step (c) comprises at least one or more CpG dinucleotide in a region which hybridizes to the methylated CpG of SDC2.
 5. The method according to claim 1, further comprising confirming whether an amplified product of methylated CpG of SDC2 gene is produced by using probe(s) capable of hybridizing with a methylated CpG of SDC2 comprising at least one or more CpG dinucleotide in a region which hybridizes to the methylated CpG of SDC2.
 6. The method according to claim 5, wherein the probe(s) comprises sequence(s) of SEQ ID NO:
 575. 7. A method for detecting CpG methylation of SDC2 (Syndecan 2) gene for a colorectal cancer diagnosis, the method comprising the steps of: (a) isolating genomic DNA from a clinical sample; (b) treating the genomic DNA from step (a) with bisulfite; and (c) measuring methylation of a CpG of the SDC2 gene in the genomic DNA treated with bisulfite according the step (b) by using a primer pair comprising a primer comprising the sequence of SEQ ID NO: 574; and a primer comprising the sequence of any one of SEQ ID NOs: 573, 577 to 594, and 612 to 635, to amplify a methylated CpG of the bisulfite-treated SDC2 gene, wherein a colorectal cancer is detected in a human subject based on increased CpG methylation of the SDC2 gene relative to that of a control.
 8. The method according to claim 7, wherein step (c) is performed by a method selected from the group consisting of PCR, methylation specific PCR, real-time methylation specific PCR, PCR using a methylated DNA-specific binding protein, quantitative PCR, pyrosequencing, and bisulfite sequencing.
 9. The method according to claim 7, wherein step (c) comprises measuring a CpG methylation of a regulatory region or intron region of SDC2 gene in the clinical sample.
 10. The method of claim 7, wherein the primer(s) of step (c) comprises at least one or more CpG dinucleotide in a region which hybridizes to the methylated CpG of SDC2.
 11. The method according to claim 7, further comprising confirming whether an amplified product of methylated CpG of SDC2 gene is produced by using probe(s) capable of hybridizing with a methylated CpG of SDC2 comprising at least one or more CpG dinucleotide in a region which hybridizes to the methylated CpG of SDC2.
 12. The method according to claim 11, wherein the probe(s) comprises sequence(s) of SEQ ID NO:
 575. 